Detailed information of regulator


Summary

Regulator ID REG0075 experimental
Regulator type protein
Regulatory pathway (from literatures) c-di-GMP regulatory pathway
KEGG pathway (determined by BlastKOALA) Two-component system (map02020); Biofilm formation - Vibrio cholerae (map05111); Flagellar assembly (map02040)
Related T6SS T6SS00014 experimental (Type i4b)
Strain Pseudomonas putida KT2440
Replicon chromosome
Sequence Protein sequence (498 a.a.); Nucleotide sequence (1494 bp)
Description RopN negatively regulates hcp1 expression at all the growth phases,and interacts a domain characteristic of FleQ.
Reference
External database links

Locus tag (Gene) PP_0952 (RopN)
Coordinates (Strand) 1094567..1096060 (-)
NCBI ID 26987688
RefSeq NC_002947
Uniprot ID RP54_PSEPK
KEGG ID ppu:PP_0952
KEGG pathway (determined by BlastKOALA) map02020; map05111; map02040
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
PP_4373 (FleQ) ↑ 26991062
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Sigma54_DBD PF04552.15 1.5e-70 337..495
Sigma54_CBD PF04963.15 6.8e-62 130..323
Sigma54_AID PF00309.22 9.6e-17 5..48
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-497 MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGEDFDNSDPMADNAENKPAAEVQDNSFQESTVSADNLEDGEWSERIPNELPVDTAWEDIYQTSASSLPSNDDDEWDFTTRTSAGESLQSHLLWQLNLAPMSDTDRLIAVTLIDSINGQGYLEDTLEEICAGFDPELDIELDEVEAVLHRIQQFEPAGVGARNLGECLLLQLRQLPATTPWMTEAKRLVTDFIDLLGSRDYSQLMRRMKIKEDELRQVIELVQSLNPRPGSQIESSEPEYVVPDVIVRKDSDRWLVELNQEAIPRLRVNPQYAGFVRRADTSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDHGDEAMKPLVLHDIAEAVGMHESTISRVTTQKYMHTPRGIYELKYFFSSHVSTSEGGECSSTAIRAIIKKLVAAENQKKPLSDSKIAGLLEAQGIQVARRTVAKYRESLGIAPSSERKRLM


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9834 - -


  Protein sequence of regulator: 498 a.a.    .

>REG0075 NC_002947:c1096060-1094567 [Pseudomonas putida KT2440]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGEDFDNSDPMADNAENKPAAEVQDNSFQ
ESTVSADNLEDGEWSERIPNELPVDTAWEDIYQTSASSLPSNDDDEWDFTTRTSAGESLQSHLLWQLNLAPMSDTDRLIA
VTLIDSINGQGYLEDTLEEICAGFDPELDIELDEVEAVLHRIQQFEPAGVGARNLGECLLLQLRQLPATTPWMTEAKRLV
TDFIDLLGSRDYSQLMRRMKIKEDELRQVIELVQSLNPRPGSQIESSEPEYVVPDVIVRKDSDRWLVELNQEAIPRLRVN
PQYAGFVRRADTSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDHGDEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIYELKYFFSSHVSTSEGGECSSTAIRAIIKKLVAAENQKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLM*

  Nucleotide sequence of regulator: 1494 bp    .

>REG0075 NC_002947:c1096060-1094567 [Pseudomonas putida KT2440]
ATGAAACCATCGCTCGTCCTAAAAATGGGCCAGCAACTGACGATGACACCTCAGTTGCAACAGGCCATCCGCCTGCTCCA
GCTTTCTACCCTGGACCTCCAACAGGAAATCCAGGAAGCGCTGGAGTCGAACCCGATGCTCGAACGTCAGGAAGACGGCG
AAGACTTCGACAACAGCGACCCGATGGCCGACAACGCCGAGAACAAGCCGGCCGCCGAAGTTCAGGACAACAGTTTCCAG
GAAAGCACCGTCAGCGCCGACAACCTGGAAGACGGTGAGTGGAGCGAGCGCATCCCCAACGAGCTCCCGGTCGATACCGC
TTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCGAGCAACGATGACGACGAATGGGACTTCACCACGCGCACAT
CGGCCGGCGAAAGCCTGCAAAGCCACCTGCTGTGGCAGTTGAACCTGGCACCGATGTCCGACACCGACCGCTTGATTGCC
GTGACCCTGATCGACAGCATCAACGGCCAGGGCTACCTGGAAGACACACTCGAAGAAATCTGCGCCGGTTTCGACCCGGA
GCTGGATATCGAACTGGACGAGGTCGAAGCGGTACTGCACCGCATCCAGCAGTTCGAGCCGGCTGGCGTTGGCGCCCGCA
ACCTCGGCGAATGCCTGTTGCTGCAACTGCGCCAGCTGCCCGCTACAACTCCGTGGATGACCGAAGCCAAGCGCCTGGTC
ACCGATTTCATCGACCTGCTCGGCAGCCGTGACTACAGCCAGCTGATGCGGCGGATGAAAATCAAGGAAGACGAGCTGCG
CCAGGTCATCGAACTGGTGCAAAGCCTTAACCCGCGCCCTGGTTCGCAGATCGAGTCCAGCGAGCCCGAGTACGTGGTGC
CCGACGTCATCGTGCGCAAGGACAGCGACCGTTGGCTGGTAGAACTGAACCAGGAAGCCATCCCACGCCTGCGCGTCAAC
CCACAATACGCCGGCTTCGTGCGCCGTGCCGACACCAGCGCCGACAACACCTTCATGCGCAACCAGCTGCAGGAAGCGCG
CTGGTTCATCAAGAGCCTGCAAAGCCGTAACGAAACCCTGATGAAGGTTGCCACGCAGATCGTCGAGCACCAGCGCGGCT
TCCTCGACCACGGCGACGAAGCGATGAAGCCGTTGGTACTGCATGACATTGCCGAAGCCGTAGGCATGCACGAATCGACC
ATTTCGCGGGTTACCACGCAGAAATACATGCACACCCCGCGTGGCATCTACGAACTGAAATACTTTTTCTCCAGCCATGT
CAGCACCTCCGAAGGCGGAGAATGCTCGTCCACGGCGATCCGCGCGATCATCAAGAAACTGGTTGCGGCGGAAAATCAGA
AAAAGCCATTGAGTGACAGCAAGATCGCTGGTTTACTGGAGGCACAAGGCATCCAGGTAGCCCGTCGCACCGTCGCCAAG
TACCGCGAGTCCCTCGGCATCGCACCGTCGAGCGAACGCAAGCGACTGATGTAG