Detailed information of regulator


Summary

Regulator ID REG0074 experimental
Regulator type protein
Regulatory pathway (from literatures) c-di-GMP regulatory pathway
KEGG pathway (determined by BlastKOALA) Two-component system (map02020); Biofilm formation - Vibrio cholerae (map05111); Biofilm formation - Pseudomonas aeruginosa (map02025); Flagellar assembly (map02040)
Related T6SS T6SS00014 experimental (Type i4b)
Strain Pseudomonas putida KT2440
Replicon chromosome
Sequence Protein sequence (492 a.a.); Nucleotide sequence (1476 bp)
Description FleQ negatively regulate hcp1 expression at all the growth phases.
Reference
External database links

Locus tag (Gene) PP_4373 (FleQ)
Coordinates (Strand) 4963882..4965357 (-)
NCBI ID 26991062
RefSeq NC_002947
Uniprot ID Q88ET0_PSEPK
KEGG ID ppu:PP_4373
KEGG pathway (determined by BlastKOALA) map02020; map05111; map02025; map02040
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
K1-T6SS (hcp1) expression ↑ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Sigma54_activat PF00158.28 4.2e-75 146..313
FleQ PF06490.13 6.8e-29 6..123
HTH_8 PF02954.21 3e-11 440..478
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-491 MWRETKILLIDDDSARRRDLAVVLNFLGEENLACASHDWQQAVEPLSSSREVLCVLIGTVNAPGNLLGLLKTVATWDEFLPVLLLGEISSAELPEDLRRRVLSNLEMPPSYSQLLDSLHRAQVYREMYDQARERGRQREPNLFRSLVGTSRAIQHVRQMMQQVADTDASVLILGESGTGKEVVARNLHYHSKRREAPFVPVNCGAIPAELLESELFGHEKGAFTGAITSRAGRFELANGGTLFLDEIGDMPLPMQVKLLRVLQERTFERVGSNKTQSIDVRIIAATHKNLETMIEDGTFREDLYYRLNVFPIEMAPLRERVEDIPLLMNELISRMEHEKRGSIRFNSASIMSLCRHGWPGNVRELANLVERMAIMHPYGVIGVSELPKKFRYVDDEDEQMVDSLRSDLEERVAINGHTPNFSNHAMLPPEGLDLKDYLGSLEQGLIQQALDDANGIVARAAERLRIRRTTLVEKMRKYGMSRQGGEDQAED


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9547 - -


  Protein sequence of regulator: 492 a.a.    .

>REG0074 NC_002947:c4965357-4963882 [Pseudomonas putida KT2440]
MWRETKILLIDDDSARRRDLAVVLNFLGEENLACASHDWQQAVEPLSSSREVLCVLIGTVNAPGNLLGLLKTVATWDEFL
PVLLLGEISSAELPEDLRRRVLSNLEMPPSYSQLLDSLHRAQVYREMYDQARERGRQREPNLFRSLVGTSRAIQHVRQMM
QQVADTDASVLILGESGTGKEVVARNLHYHSKRREAPFVPVNCGAIPAELLESELFGHEKGAFTGAITSRAGRFELANGG
TLFLDEIGDMPLPMQVKLLRVLQERTFERVGSNKTQSIDVRIIAATHKNLETMIEDGTFREDLYYRLNVFPIEMAPLRER
VEDIPLLMNELISRMEHEKRGSIRFNSASIMSLCRHGWPGNVRELANLVERMAIMHPYGVIGVSELPKKFRYVDDEDEQM
VDSLRSDLEERVAINGHTPNFSNHAMLPPEGLDLKDYLGSLEQGLIQQALDDANGIVARAAERLRIRRTTLVEKMRKYGM
SRQGGEDQAED*

  Nucleotide sequence of regulator: 1476 bp    .

>REG0074 NC_002947:c4965357-4963882 [Pseudomonas putida KT2440]
ATGTGGCGTGAAACCAAGATTCTGCTGATCGATGACGACAGCGCGCGCCGCCGCGACCTTGCGGTGGTGCTGAACTTCCT
CGGCGAAGAAAACCTCGCGTGTGCCAGCCATGACTGGCAGCAGGCGGTCGAGCCATTGTCCTCGAGCCGCGAAGTGCTGT
GCGTGCTCATCGGCACCGTGAATGCTCCGGGCAATCTGCTTGGGCTGCTTAAGACAGTGGCTACCTGGGATGAGTTCCTT
CCGGTTTTGCTTTTAGGTGAAATTTCTTCCGCCGAGCTGCCGGAAGACCTTCGCCGCCGGGTGCTTTCCAACCTGGAAAT
GCCGCCAAGCTACAGCCAATTGCTGGACTCGCTGCACCGTGCCCAGGTCTACCGCGAAATGTATGACCAGGCGCGCGAGC
GGGGTCGCCAGCGCGAACCCAATCTGTTCCGCAGCCTGGTTGGCACCAGTCGTGCCATTCAGCACGTGCGGCAGATGATG
CAGCAAGTGGCGGATACCGACGCCAGCGTGCTGATCCTGGGTGAGTCCGGCACCGGCAAGGAAGTGGTAGCGCGCAACCT
GCACTACCATTCCAAGCGCCGCGAAGCGCCGTTCGTGCCGGTCAACTGTGGCGCGATCCCGGCCGAGCTGCTGGAAAGCG
AACTGTTCGGCCACGAGAAGGGCGCCTTTACCGGGGCCATCACCAGCCGTGCCGGGCGTTTCGAACTGGCCAACGGCGGT
ACCCTGTTCCTCGACGAAATCGGCGACATGCCGTTGCCGATGCAGGTCAAGCTGCTGCGTGTGCTGCAGGAGCGTACCTT
CGAGCGCGTGGGTAGCAACAAGACCCAAAGCATCGACGTGCGCATCATCGCTGCGACGCACAAGAACCTCGAAACCATGA
TCGAGGACGGCACCTTCCGCGAAGACCTGTACTACCGCCTGAACGTGTTCCCTATCGAAATGGCGCCGCTGCGTGAGCGG
GTGGAAGACATCCCGTTGCTGATGAACGAGCTGATTTCGCGCATGGAGCACGAGAAGCGCGGTTCCATTCGCTTCAATTC
CGCCTCGATCATGTCGCTGTGTCGTCACGGCTGGCCGGGCAACGTACGCGAGCTGGCCAACCTGGTCGAGCGCATGGCGA
TCATGCACCCGTACGGGGTCATTGGCGTGTCGGAACTGCCGAAGAAATTCCGCTATGTCGATGACGAAGACGAGCAGATG
GTCGACAGCCTGCGCAGCGACCTGGAAGAGCGCGTGGCGATCAATGGCCACACACCAAACTTCAGCAACCACGCCATGCT
GCCGCCCGAAGGCCTGGACCTCAAGGACTACCTGGGTAGCCTGGAGCAGGGCCTGATCCAGCAGGCGCTGGACGATGCCA
ACGGTATCGTTGCGCGCGCGGCTGAACGTTTGCGTATTCGCCGTACCACCCTGGTCGAGAAAATGCGCAAGTACGGCATG
AGCCGCCAGGGCGGTGAGGACCAGGCGGAGGATTGA