Detailed information of Recipient susceptibility factor


Summary

ID RSF000053 experimental
Strain Neisseria cinerea isolate CCUG346T (346T)
Replicon chromosome
Sequence Protein sequence (175 a.a.); Nucleotide sequence (525 bp)
Response to T6SS attack sensitive
Known or putative function Type IV pili (Tfp)
Description Tfp influence the outcome of T6SS-mediated antagonism through structuring and partitioning bacteria in mixed microcolonies. The non-piliated prey strain (346TDnte/i3-5DpilE1/ 2_sfCherry; red) segregates to the periphery of the colony, in this location the prey strain escapes T6SS-mediated assault and dominates the expanding colony and when the prey is piliated, pilus-mediated cell interactions prevent displacement of cells to the expanding front so the susceptible strain (Tfp-expressing 346TDnte/i3-5_sfCherry Tfp+, red) is outcompeted by the T6SS+ strain (Tfp-expressing 346T_sfGfp Tfp+, green).
Reference
experimental Experimental investigation has been performed.

External database links

Locus tag (Gene) pilE2
Coordinates (Strand) 1475801..1476325 (+)
NCBI ID
GenBank 346T
Uniprot ID -
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Pilin PF00114.21 1.6e-13 62..173
N_methyl PF07963.14 3.9e-08 31..54

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-31 MMAEKLGGGGNIDYNHADKLTIKNINMKNKP
Transmembrane helix 32-54 YGFTLIELMIVTAILGILTMLAL
Outside 55-174 PAYQDYAARAQVAETYYLLDELKTEIGIYASIYNHLPDAAEVSPSGGIGSAAARMGGTYIRHGGVTVEADTAKISVPFDKGYHKGKMLTLSPTRDSESGIITWVCGGTIEPRSLPAVCRR



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9902 - -



  Protein sequence: 175 a.a.    .

>RSF000053 346T:1475801-1476325 [synthetic DNA construct]
MMAEKLGGGGNIDYNHADKLTIKNINMKNKPYGFTLIELMIVTAILGILTMLALPAYQDYAARAQVAETYYLLDELKTEI
GIYASIYNHLPDAAEVSPSGGIGSAAARMGGTYIRHGGVTVEADTAKISVPFDKGYHKGKMLTLSPTRDSESGIITWVCG
GTIEPRSLPAVCRR*

  Nucleotide sequence: 525 bp    .

>RSF000053 346T:1475801-1476325 [synthetic DNA construct]
ATGATGGCGGAAAAGTTGGGGGGCGGCGGGAATATCGATTATAATCATGCGGATAAACTAACCATAAAAAATATAAATAT
GAAGAACAAACCTTACGGGTTTACGCTGATCGAGCTGATGATCGTAACGGCAATCTTGGGGATACTGACCATGCTCGCCC
TGCCTGCCTATCAAGACTATGCGGCTAGGGCGCAGGTTGCCGAAACCTACTATCTGTTGGATGAGCTGAAAACCGAAATC
GGAATTTATGCCTCTATTTACAACCATCTGCCCGACGCGGCGGAAGTTTCCCCGTCGGGCGGCATCGGCAGCGCGGCCGC
CAGGATGGGCGGGACGTATATCCGGCATGGCGGCGTTACCGTCGAGGCGGATACTGCGAAAATTTCCGTACCCTTCGATA
AAGGGTATCACAAGGGCAAAATGCTGACGCTGTCGCCCACGCGCGATAGCGAAAGCGGCATCATTACCTGGGTGTGCGGC
GGGACGATAGAGCCGAGGTCGCTGCCTGCCGTCTGCCGCCGTTAA