Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000053  |
| Strain | Neisseria cinerea isolate CCUG346T (346T) |
| Replicon | chromosome |
| Sequence | Protein sequence (175 a.a.); Nucleotide sequence (525 bp) |
| Response to T6SS attack | sensitive |
| Known or putative function | Type IV pili (Tfp) |
| Description | Tfp influence the outcome of T6SS-mediated antagonism through structuring and partitioning bacteria in mixed microcolonies. The non-piliated prey strain (346TDnte/i3-5DpilE1/ 2_sfCherry; red) segregates to the periphery of the colony, in this location the prey strain escapes T6SS-mediated assault and dominates the expanding colony and when the prey is piliated, pilus-mediated cell interactions prevent displacement of cells to the expanding front so the susceptible strain (Tfp-expressing 346TDnte/i3-5_sfCherry Tfp+, red) is outcompeted by the T6SS+ strain (Tfp-expressing 346T_sfGfp Tfp+, green). |
| Reference |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | pilE2 |
| Coordinates (Strand) | 1475801..1476325 (+) |
| NCBI ID | |
| GenBank | 346T |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Pilin | PF00114.21 | 1.6e-13 | 62..173 |
| N_methyl | PF07963.14 | 3.9e-08 | 31..54 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-31 | MMAEKLGGGGNIDYNHADKLTIKNINMKNKP |
| Transmembrane helix | 32-54 | YGFTLIELMIVTAILGILTMLAL |
| Outside | 55-174 | PAYQDYAARAQVAETYYLLDELKTEIGIYASIYNHLPDAAEVSPSGGIGSAAARMGGTYIRHGGVTVEADTAKISVPFDKGYHKGKMLTLSPTRDSESGIITWVCGGTIEPRSLPAVCRR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9902 | - | - |
Protein sequence: 175 a.a. .
>RSF000053 346T:1475801-1476325 [synthetic DNA construct]
MMAEKLGGGGNIDYNHADKLTIKNINMKNKPYGFTLIELMIVTAILGILTMLALPAYQDYAARAQVAETYYLLDELKTEI
GIYASIYNHLPDAAEVSPSGGIGSAAARMGGTYIRHGGVTVEADTAKISVPFDKGYHKGKMLTLSPTRDSESGIITWVCG
GTIEPRSLPAVCRR*
MMAEKLGGGGNIDYNHADKLTIKNINMKNKPYGFTLIELMIVTAILGILTMLALPAYQDYAARAQVAETYYLLDELKTEI
GIYASIYNHLPDAAEVSPSGGIGSAAARMGGTYIRHGGVTVEADTAKISVPFDKGYHKGKMLTLSPTRDSESGIITWVCG
GTIEPRSLPAVCRR*
Nucleotide sequence: 525 bp .
>RSF000053 346T:1475801-1476325 [synthetic DNA construct]
ATGATGGCGGAAAAGTTGGGGGGCGGCGGGAATATCGATTATAATCATGCGGATAAACTAACCATAAAAAATATAAATAT
GAAGAACAAACCTTACGGGTTTACGCTGATCGAGCTGATGATCGTAACGGCAATCTTGGGGATACTGACCATGCTCGCCC
TGCCTGCCTATCAAGACTATGCGGCTAGGGCGCAGGTTGCCGAAACCTACTATCTGTTGGATGAGCTGAAAACCGAAATC
GGAATTTATGCCTCTATTTACAACCATCTGCCCGACGCGGCGGAAGTTTCCCCGTCGGGCGGCATCGGCAGCGCGGCCGC
CAGGATGGGCGGGACGTATATCCGGCATGGCGGCGTTACCGTCGAGGCGGATACTGCGAAAATTTCCGTACCCTTCGATA
AAGGGTATCACAAGGGCAAAATGCTGACGCTGTCGCCCACGCGCGATAGCGAAAGCGGCATCATTACCTGGGTGTGCGGC
GGGACGATAGAGCCGAGGTCGCTGCCTGCCGTCTGCCGCCGTTAA
ATGATGGCGGAAAAGTTGGGGGGCGGCGGGAATATCGATTATAATCATGCGGATAAACTAACCATAAAAAATATAAATAT
GAAGAACAAACCTTACGGGTTTACGCTGATCGAGCTGATGATCGTAACGGCAATCTTGGGGATACTGACCATGCTCGCCC
TGCCTGCCTATCAAGACTATGCGGCTAGGGCGCAGGTTGCCGAAACCTACTATCTGTTGGATGAGCTGAAAACCGAAATC
GGAATTTATGCCTCTATTTACAACCATCTGCCCGACGCGGCGGAAGTTTCCCCGTCGGGCGGCATCGGCAGCGCGGCCGC
CAGGATGGGCGGGACGTATATCCGGCATGGCGGCGTTACCGTCGAGGCGGATACTGCGAAAATTTCCGTACCCTTCGATA
AAGGGTATCACAAGGGCAAAATGCTGACGCTGTCGCCCACGCGCGATAGCGAAAGCGGCATCATTACCTGGGTGTGCGGC
GGGACGATAGAGCCGAGGTCGCTGCCTGCCGTCTGCCGCCGTTAA