Detailed information of Recipient susceptibility factor


Summary

ID RSF000052 experimental
Strain Neisseria meningitidis MC58
Replicon chromosome
Sequence Protein sequence (215 a.a.); Nucleotide sequence (645 bp)
Response to T6SS attack protective
Known or putative function capsule protection
Description Using a capsule-null strain (ΔsiaD) in competition assays with wild-type N. cinerea 346T or the T6SS mutant, we found reduced survival of the unencapsulated mutant compared to the wild-type . Therefore, the meningococcal capsule protects bacteria against T6SS attack.
Reference
experimental Experimental investigation has been performed.

External database links

Locus tag (Gene) NMB_RS00365 (siaD)
Coordinates (Strand) 76646..77290 (-)
NCBI ID
RefSeq NC_003112
Uniprot ID -
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
A-2_8-polyST PF07388.13 3.7e-26 35..214

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-215 MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQLNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNITPKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAPLLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFA



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9923 - -



  Protein sequence: 215 a.a.    .

>RSF000052 NC_003112:c77290-76646 [Neisseria meningitidis MC58]
MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQLNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQS
ANKNLFESIYLFELPRSPNNITPKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFA

  Nucleotide sequence: 645 bp    .

>RSF000052 NC_003112:c77290-76646 [Neisseria meningitidis MC58]
ATGCTAAAGAAAATAAAAAAAGCTCTTTTTCAGCCTAAAAAGTTTTTTCAAGATTCAATGTGGTTGACAACATCTCCATT
TTATCTTACCCCCCCACGTAACAATTTATTTGTCATATCTAATTTAGGTCAGCTTAACCAAGTCCAAAGCCTAATTAAAA
TACAAAAATTAACCAATAATTTACTAGTAATTTTATATACTTCTAAAAACTTAAAAATGCCTAAGTTAGTTCATCAATCA
GCTAACAAGAATCTATTTGAATCTATTTATCTATTTGAGCTTCCTAGAAGCCCTAATAATATAACTCCTAAAAAATTACT
TTATATTTATAGAAGTTACAAAAAAATCCTTAATATTATACAGCCTGCTCATCTCTATATGCTGTCTTTTACAGGCCACT
ACTCCTATCTGATTAGTATTGCAAAAAAAAAGAATATTACGACTCATTTAATTGATGAAGGGACTGGAACATATGCTCCT
TTATTAGAATCATTTTCATATCATCCAACAAAATTAGAACGTTATTTGATTGGAAATAATCTTAATATTAAAGGATATAT
AGATCATTTTGACATATTGCATGTCCCCTTTCCTGAATATGCTAAAAAAATATTTAATGCAAAAAAATATAACCGGTTTT
TTGCC