Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000052  |
| Strain | Neisseria meningitidis MC58 |
| Replicon | chromosome |
| Sequence | Protein sequence (215 a.a.); Nucleotide sequence (645 bp) |
| Response to T6SS attack | protective |
| Known or putative function | capsule protection |
| Description | Using a capsule-null strain (ΔsiaD) in competition assays with wild-type N. cinerea 346T or the T6SS mutant, we found reduced survival of the unencapsulated mutant compared to the wild-type . Therefore, the meningococcal capsule protects bacteria against T6SS attack. |
| Reference |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | NMB_RS00365 (siaD) |
| Coordinates (Strand) | 76646..77290 (-) |
| NCBI ID | |
| RefSeq | NC_003112 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| A-2_8-polyST | PF07388.13 | 3.7e-26 | 35..214 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-215 | MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQLNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNITPKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAPLLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9923 | - | - |
Protein sequence: 215 a.a. .
>RSF000052 NC_003112:c77290-76646 [Neisseria meningitidis MC58]
MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQLNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQS
ANKNLFESIYLFELPRSPNNITPKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFA
MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQLNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQS
ANKNLFESIYLFELPRSPNNITPKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFA
Nucleotide sequence: 645 bp .
>RSF000052 NC_003112:c77290-76646 [Neisseria meningitidis MC58]
ATGCTAAAGAAAATAAAAAAAGCTCTTTTTCAGCCTAAAAAGTTTTTTCAAGATTCAATGTGGTTGACAACATCTCCATT
TTATCTTACCCCCCCACGTAACAATTTATTTGTCATATCTAATTTAGGTCAGCTTAACCAAGTCCAAAGCCTAATTAAAA
TACAAAAATTAACCAATAATTTACTAGTAATTTTATATACTTCTAAAAACTTAAAAATGCCTAAGTTAGTTCATCAATCA
GCTAACAAGAATCTATTTGAATCTATTTATCTATTTGAGCTTCCTAGAAGCCCTAATAATATAACTCCTAAAAAATTACT
TTATATTTATAGAAGTTACAAAAAAATCCTTAATATTATACAGCCTGCTCATCTCTATATGCTGTCTTTTACAGGCCACT
ACTCCTATCTGATTAGTATTGCAAAAAAAAAGAATATTACGACTCATTTAATTGATGAAGGGACTGGAACATATGCTCCT
TTATTAGAATCATTTTCATATCATCCAACAAAATTAGAACGTTATTTGATTGGAAATAATCTTAATATTAAAGGATATAT
AGATCATTTTGACATATTGCATGTCCCCTTTCCTGAATATGCTAAAAAAATATTTAATGCAAAAAAATATAACCGGTTTT
TTGCC
ATGCTAAAGAAAATAAAAAAAGCTCTTTTTCAGCCTAAAAAGTTTTTTCAAGATTCAATGTGGTTGACAACATCTCCATT
TTATCTTACCCCCCCACGTAACAATTTATTTGTCATATCTAATTTAGGTCAGCTTAACCAAGTCCAAAGCCTAATTAAAA
TACAAAAATTAACCAATAATTTACTAGTAATTTTATATACTTCTAAAAACTTAAAAATGCCTAAGTTAGTTCATCAATCA
GCTAACAAGAATCTATTTGAATCTATTTATCTATTTGAGCTTCCTAGAAGCCCTAATAATATAACTCCTAAAAAATTACT
TTATATTTATAGAAGTTACAAAAAAATCCTTAATATTATACAGCCTGCTCATCTCTATATGCTGTCTTTTACAGGCCACT
ACTCCTATCTGATTAGTATTGCAAAAAAAAAGAATATTACGACTCATTTAATTGATGAAGGGACTGGAACATATGCTCCT
TTATTAGAATCATTTTCATATCATCCAACAAAATTAGAACGTTATTTGATTGGAAATAATCTTAATATTAAAGGATATAT
AGATCATTTTGACATATTGCATGTCCCCTTTCCTGAATATGCTAAAAAAATATTTAATGCAAAAAAATATAACCGGTTTT
TTGCC