Detailed information of Recipient susceptibility factor


Summary

ID RSF000051 experimental
Strain Serratia marcescens subsp. marcescens Db11
Replicon chromosome
Sequence Protein sequence (214 a.a.); Nucleotide sequence (642 bp)
Response to T6SS attack sensitive
Known or putative function Effector activator
Description The presence of DsbA in target cells is essential for the activation of incoming periplasmic-acting effectors Ssp2 and Ssp4, and is required in the secreting cell for full T6SS function and acts on several components of the secretion machinery
Reference
experimental Experimental investigation has been performed.

External database links

Locus tag (Gene) SMDB11_0239 (DsbA2)
Coordinates (Strand) 265001..265642 (+)
NCBI ID 560172104
GenBank HG326223
Uniprot ID UPI0003C9AE1C
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DSBA PF01323.22 4.2e-19 46..200

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-213 MLAKVKRSFAAAVVLMLALPAVQAADYRAGEQYTRLDKPVAAAPAVVEFFSFYCGPCYQFAETYRVGSTVAQALPAGEKVTKYHVSLMGKLGNELTEAWAVATVLGVEDKIEGAMFDAVQKQRAVNGADDIQRVFTAAGIDAATYENARHSLLVKGLIAKQNEAVKAFEVRGTPSFYVAGKYKIDNAGMASTSVEGYAKEYAAVVRYLLDKQP



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9968 pos: 24-25. VQA-AD. MLAKVKRSFAAAVVLMLALPAVQA



  Protein sequence: 214 a.a.    .

>RSF000051 HG326223:265001-265642 [Serratia marcescens subsp. marcescens Db11]
MLAKVKRSFAAAVVLMLALPAVQAADYRAGEQYTRLDKPVAAAPAVVEFFSFYCGPCYQFAETYRVGSTVAQALPAGEKV
TKYHVSLMGKLGNELTEAWAVATVLGVEDKIEGAMFDAVQKQRAVNGADDIQRVFTAAGIDAATYENARHSLLVKGLIAK
QNEAVKAFEVRGTPSFYVAGKYKIDNAGMASTSVEGYAKEYAAVVRYLLDKQP*

  Nucleotide sequence: 642 bp    .

>RSF000051 HG326223:265001-265642 [Serratia marcescens subsp. marcescens Db11]
ATGTTGGCAAAAGTTAAGCGTTCGTTCGCCGCCGCCGTCGTATTGATGTTGGCGCTGCCTGCGGTTCAGGCCGCGGATTA
CCGTGCGGGCGAGCAATACACCCGGCTGGATAAGCCGGTGGCCGCCGCGCCGGCGGTGGTGGAGTTCTTCTCCTTCTACT
GCGGCCCTTGCTATCAGTTCGCCGAGACCTATCGCGTAGGCAGTACCGTGGCGCAGGCGCTGCCCGCCGGTGAGAAAGTC
ACCAAATATCACGTCAGCCTGATGGGCAAACTGGGCAATGAGCTGACCGAAGCCTGGGCGGTGGCGACGGTGCTGGGCGT
GGAAGACAAGATTGAAGGGGCGATGTTCGACGCGGTGCAGAAACAGCGCGCCGTCAACGGCGCCGACGATATTCAGCGGG
TGTTTACGGCGGCCGGCATCGATGCCGCTACCTATGAAAACGCGCGCCACAGCCTGCTGGTCAAAGGATTGATCGCCAAG
CAGAACGAAGCGGTGAAAGCATTCGAGGTGCGCGGCACCCCGTCGTTCTACGTGGCAGGCAAATACAAGATAGACAATGC
CGGCATGGCCAGCACCAGCGTTGAAGGCTACGCCAAAGAGTACGCCGCGGTGGTGCGTTACCTGTTGGACAAACAGCCGT
AA