Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000051  |
| Strain | Serratia marcescens subsp. marcescens Db11 |
| Replicon | chromosome |
| Sequence | Protein sequence (214 a.a.); Nucleotide sequence (642 bp) |
| Response to T6SS attack | sensitive |
| Known or putative function | Effector activator |
| Description | The presence of DsbA in target cells is essential for the activation of incoming periplasmic-acting effectors Ssp2 and Ssp4, and is required in the secreting cell for full T6SS function and acts on several components of the secretion machinery |
| Reference |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | SMDB11_0239 (DsbA2) |
| Coordinates (Strand) | 265001..265642 (+) |
| NCBI ID | 560172104 |
| GenBank | HG326223 |
| Uniprot ID | UPI0003C9AE1C |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DSBA | PF01323.22 | 4.2e-19 | 46..200 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-213 | MLAKVKRSFAAAVVLMLALPAVQAADYRAGEQYTRLDKPVAAAPAVVEFFSFYCGPCYQFAETYRVGSTVAQALPAGEKVTKYHVSLMGKLGNELTEAWAVATVLGVEDKIEGAMFDAVQKQRAVNGADDIQRVFTAAGIDAATYENARHSLLVKGLIAKQNEAVKAFEVRGTPSFYVAGKYKIDNAGMASTSVEGYAKEYAAVVRYLLDKQP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9968 | pos: 24-25. VQA-AD. | MLAKVKRSFAAAVVLMLALPAVQA |
Protein sequence: 214 a.a. .
>RSF000051 HG326223:265001-265642 [Serratia marcescens subsp. marcescens Db11]
MLAKVKRSFAAAVVLMLALPAVQAADYRAGEQYTRLDKPVAAAPAVVEFFSFYCGPCYQFAETYRVGSTVAQALPAGEKV
TKYHVSLMGKLGNELTEAWAVATVLGVEDKIEGAMFDAVQKQRAVNGADDIQRVFTAAGIDAATYENARHSLLVKGLIAK
QNEAVKAFEVRGTPSFYVAGKYKIDNAGMASTSVEGYAKEYAAVVRYLLDKQP*
MLAKVKRSFAAAVVLMLALPAVQAADYRAGEQYTRLDKPVAAAPAVVEFFSFYCGPCYQFAETYRVGSTVAQALPAGEKV
TKYHVSLMGKLGNELTEAWAVATVLGVEDKIEGAMFDAVQKQRAVNGADDIQRVFTAAGIDAATYENARHSLLVKGLIAK
QNEAVKAFEVRGTPSFYVAGKYKIDNAGMASTSVEGYAKEYAAVVRYLLDKQP*
Nucleotide sequence: 642 bp .
>RSF000051 HG326223:265001-265642 [Serratia marcescens subsp. marcescens Db11]
ATGTTGGCAAAAGTTAAGCGTTCGTTCGCCGCCGCCGTCGTATTGATGTTGGCGCTGCCTGCGGTTCAGGCCGCGGATTA
CCGTGCGGGCGAGCAATACACCCGGCTGGATAAGCCGGTGGCCGCCGCGCCGGCGGTGGTGGAGTTCTTCTCCTTCTACT
GCGGCCCTTGCTATCAGTTCGCCGAGACCTATCGCGTAGGCAGTACCGTGGCGCAGGCGCTGCCCGCCGGTGAGAAAGTC
ACCAAATATCACGTCAGCCTGATGGGCAAACTGGGCAATGAGCTGACCGAAGCCTGGGCGGTGGCGACGGTGCTGGGCGT
GGAAGACAAGATTGAAGGGGCGATGTTCGACGCGGTGCAGAAACAGCGCGCCGTCAACGGCGCCGACGATATTCAGCGGG
TGTTTACGGCGGCCGGCATCGATGCCGCTACCTATGAAAACGCGCGCCACAGCCTGCTGGTCAAAGGATTGATCGCCAAG
CAGAACGAAGCGGTGAAAGCATTCGAGGTGCGCGGCACCCCGTCGTTCTACGTGGCAGGCAAATACAAGATAGACAATGC
CGGCATGGCCAGCACCAGCGTTGAAGGCTACGCCAAAGAGTACGCCGCGGTGGTGCGTTACCTGTTGGACAAACAGCCGT
AA
ATGTTGGCAAAAGTTAAGCGTTCGTTCGCCGCCGCCGTCGTATTGATGTTGGCGCTGCCTGCGGTTCAGGCCGCGGATTA
CCGTGCGGGCGAGCAATACACCCGGCTGGATAAGCCGGTGGCCGCCGCGCCGGCGGTGGTGGAGTTCTTCTCCTTCTACT
GCGGCCCTTGCTATCAGTTCGCCGAGACCTATCGCGTAGGCAGTACCGTGGCGCAGGCGCTGCCCGCCGGTGAGAAAGTC
ACCAAATATCACGTCAGCCTGATGGGCAAACTGGGCAATGAGCTGACCGAAGCCTGGGCGGTGGCGACGGTGCTGGGCGT
GGAAGACAAGATTGAAGGGGCGATGTTCGACGCGGTGCAGAAACAGCGCGCCGTCAACGGCGCCGACGATATTCAGCGGG
TGTTTACGGCGGCCGGCATCGATGCCGCTACCTATGAAAACGCGCGCCACAGCCTGCTGGTCAAAGGATTGATCGCCAAG
CAGAACGAAGCGGTGAAAGCATTCGAGGTGCGCGGCACCCCGTCGTTCTACGTGGCAGGCAAATACAAGATAGACAATGC
CGGCATGGCCAGCACCAGCGTTGAAGGCTACGCCAAAGAGTACGCCGCGGTGGTGCGTTACCTGTTGGACAAACAGCCGT
AA