Detailed information of Recipient susceptibility factor


Summary

ID RSF000034 experimental
Strain Vibrio cholerae V52
Replicon chromosome
Sequence Protein sequence (373 a.a.); Nucleotide sequence (1119 bp)
Response to T6SS attack protective
Known or putative function Vibrio polysaccharide (VPS) expression
Description V52 lacking immunity genes or facing a T6SS attack by A. dhakensis were indeed highlysensitive to killing when pre-cultured at 37°C but were impressively protected after growth at 30°C. This protection was largely dependent on VPS, as the equivalent ΔvpsA mutants showed significantly reduced survival.
Reference
experimental Experimental investigation has been performed.
External database links

Locus tag (Gene) VCV52_RS11930 (vpsA)
Coordinates (Strand) 316448..317566 (-)
NCBI ID 446679410
RefSeq NZ_KQ410501
Uniprot ID Q9KTI5_VIBCH, A0A0X1KV75_VIBCO, A0A0H3AKX9_VIBC3, A0A0K9URX0_VIBCL, C3LTG6_VIBCM, A0A0E4CCM6_VIBCL
KEGG ID vcm:VCM66_0874, vch:VC0917, vcq:EN18_07940, vcz:VAB027_1608, vco:VC0395_A0441
PDB ID 3DZC
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Epimerase_2 PF02350.21 1.4e-122 24..370
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-372 VKKVLIVFGTRPEAIKMAPLVQQLCQDNRFVAKVCVTGQHREMLDQVLELFSITPDFDLNIMEPGQTLNGVTSKILLGMQQVLSSEQPDVVLVHGDTATTFAASLAAYYQQIPVGHVEAGLRTGNIYSPWPEEGNRKLTAALTQYHFAPTDTSRANLLQENYNAENIFVTGNTVIDALLAVREKIHTDMDLQATLESQFPMLDASKKLILVTGHRRESFGGGFERICQALITTAEQHPECQILYPVHLNPNVREPVNKLLKGVSNIVLIEPQQYLPFVYLMDRAHIILTDSGGIQEEAPSLGKPVLVMRETTERPEAVAAGTVKLVGTNQQQICDALSLLLTDPQAYQAMSQAHNPYGDGKACQRIADILAK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9665 - -


  Protein sequence: 373 a.a.    .

>RSF000034 NZ_KQ410501:c317566-316448 [Vibrio cholerae V52]
VKKVLIVFGTRPEAIKMAPLVQQLCQDNRFVAKVCVTGQHREMLDQVLELFSITPDFDLNIMEPGQTLNGVTSKILLGMQ
QVLSSEQPDVVLVHGDTATTFAASLAAYYQQIPVGHVEAGLRTGNIYSPWPEEGNRKLTAALTQYHFAPTDTSRANLLQE
NYNAENIFVTGNTVIDALLAVREKIHTDMDLQATLESQFPMLDASKKLILVTGHRRESFGGGFERICQALITTAEQHPEC
QILYPVHLNPNVREPVNKLLKGVSNIVLIEPQQYLPFVYLMDRAHIILTDSGGIQEEAPSLGKPVLVMRETTERPEAVAA
GTVKLVGTNQQQICDALSLLLTDPQAYQAMSQAHNPYGDGKACQRIADILAK*

  Nucleotide sequence: 1119 bp    .

>RSF000034 NZ_KQ410501:c317566-316448 [Vibrio cholerae V52]
GTGAAAAAAGTTCTCATCGTATTTGGTACGCGCCCAGAGGCAATCAAAATGGCACCTCTGGTGCAGCAACTCTGTCAAGA
CAATCGTTTTGTCGCCAAGGTATGTGTAACGGGCCAACACCGTGAGATGTTAGATCAGGTATTAGAGCTGTTTTCGATTA
CGCCTGATTTTGATCTCAACATCATGGAGCCTGGGCAAACACTCAATGGCGTGACCAGCAAAATTTTGCTCGGTATGCAG
CAAGTGTTGAGTAGTGAACAGCCAGATGTGGTTTTAGTGCATGGTGATACCGCTACCACGTTTGCTGCCTCTTTAGCCGC
GTATTATCAACAAATTCCGGTAGGTCATGTTGAAGCGGGTTTGAGAACGGGCAATATCTATTCCCCTTGGCCTGAAGAGG
GCAACCGCAAACTCACGGCCGCACTCACGCAGTACCACTTTGCACCTACAGATACTTCGCGTGCCAATCTGCTGCAAGAA
AACTACAACGCAGAGAATATTTTCGTCACTGGTAATACGGTGATTGATGCGTTGTTGGCTGTGCGTGAAAAGATCCATAC
CGATATGGATCTGCAAGCCACTCTAGAGAGTCAGTTTCCAATGCTTGATGCGAGCAAAAAGCTGATTCTGGTAACAGGAC
ACCGCCGTGAAAGTTTTGGTGGTGGCTTTGAGCGTATTTGCCAAGCGCTGATCACTACCGCAGAGCAGCATCCCGAATGC
CAAATTTTGTATCCCGTGCATCTCAATCCGAATGTGCGTGAACCGGTCAATAAGCTCCTAAAAGGTGTCAGCAATATTGT
CTTGATTGAACCGCAGCAGTATTTGCCTTTCGTTTACTTGATGGATCGCGCACACATTATTTTGACTGACTCTGGCGGCA
TTCAAGAAGAGGCGCCCTCGCTGGGTAAGCCAGTGCTGGTGATGCGTGAAACGACTGAGCGCCCTGAAGCGGTGGCTGCG
GGCACGGTGAAATTAGTCGGTACCAACCAACAACAAATTTGTGATGCGTTAAGCTTACTACTGACTGACCCACAGGCTTA
TCAAGCCATGAGTCAGGCACACAACCCTTATGGGGATGGTAAAGCCTGTCAACGTATTGCGGACATTTTAGCGAAATAG