Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000033  |
| Strain | Vibrio cholerae V52 |
| Replicon | chromosome |
| Sequence | Protein sequence (230 a.a.); Nucleotide sequence (690 bp) |
| Response to T6SS attack | protective |
| Known or putative function | cell-wall damage response |
| Description | ΔwigR V52 was greatly impaired in its ability to kill E. coli prey. This reduced killing activity was reflected at both the protein and messenger RNA levels, as ΔwigR V52 produced fewer T6SS needles and had reduced expression of T6SS genes, hcp and vipA, compared with the WT. |
| Reference | [1] Hersch SJ, Watanabe N, Stietz MS, et al. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins. Nat Microbiol. 2020 May;5(5):706-714. doi: 10.1038/s41564-020-0672-6. Epub 2020 Feb 24. PMID: 32094588 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336 |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | VCV52_0688 (wigR/phoB/vxrB) |
| Coordinates (Strand) | 201806..202495 (-) |
| NCBI ID | 446013262 |
| RefSeq | NZ_KQ410503 |
| Uniprot ID | Q9KU11_VIBCH, A0A0X1KUP3_VIBCO, Q19LH9_VIBC3, D7HER4_VIBCL, A0A0K9UVD4_VIBCL, A0A0H3PW59_VIBCL, C3LSX2_VIBCM, A0A085TF90_VIBCL, D2YHP6_VIBMI, A0A1D8SDF5_VIBMI |
| KEGG ID | vcq:EN18_07005, vcx:VAA049_2973, vcm:VCM66_0677, vch:VC0719, vcz:VAB027_1380, vco:VC0395_A0253 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Response_reg | PF00072.26 | 5.7e-29 | 5..116 |
| Trans_reg_C | PF00486.30 | 1.4e-28 | 151..225 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-229 | MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDSAMSKLAEPFPDLVLLDWMLPGGSGINLIKHMKREEMTRNIPVVMLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTANDQPLDMGPTEFKMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9966 | - | - |
Protein sequence: 230 a.a. .
>RSF000033 NZ_KQ410503:c202495-201806 [Vibrio cholerae V52]
MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDSAMSKLAEPFPDLVLLDWMLPGGSGINLIKHMKREEMTRNIPVV
MLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTANDQPLDMGPTEF
KMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA*
MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDSAMSKLAEPFPDLVLLDWMLPGGSGINLIKHMKREEMTRNIPVV
MLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTANDQPLDMGPTEF
KMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA*
Nucleotide sequence: 690 bp .
>RSF000033 NZ_KQ410503:c202495-201806 [Vibrio cholerae V52]
ATGTCTAGAAGGATTCTGGTTGTTGAAGACGAAGCACCGATTCGTGAAATGTTGTGTTTCGTGCTTGAACAAAAGGGCTA
TCAGGCGGTTGAGGCTGAAGATTATGATTCGGCGATGAGTAAGCTGGCTGAGCCTTTTCCTGATCTCGTCCTGCTTGACT
GGATGTTGCCTGGTGGTAGTGGCATCAACCTCATCAAGCATATGAAGCGCGAAGAGATGACGCGCAACATTCCTGTTGTG
ATGCTCACCGCCCGTGGTGAAGAAGAAGATAAAGTTCGCGGCTTAGAAGTGGGCGCGGATGATTACATTACCAAACCGTT
TTCACCTAAAGAGTTGGTTGCCCGTTTAAAAGCGGTGATCCGCCGTGTCACTCCGACGGCGTTGGAGGATGTGATTGATG
TTCAAGGTTTGAAGTTAGATCCGGTCTCACACCGTGTGACCGCTAACGATCAGCCGTTGGATATGGGACCGACTGAGTTC
AAGATGTTACACTTCTTCATGACACACCAAGAGCGTGTGTATAGCCGCGAGCAGCTACTCAATAATGTTTGGGGCACCAA
CGTGTATGTGGAAGATCGCACGGTAGACGTCCACATTCGTCGTCTACGTAAAGCCTTAGAAGACGCTGGACATGATAAGC
TGATCCAGACGGTGCGCGGCGCGGGGTATCGTTTTTCAACCAAAGCCTAA
ATGTCTAGAAGGATTCTGGTTGTTGAAGACGAAGCACCGATTCGTGAAATGTTGTGTTTCGTGCTTGAACAAAAGGGCTA
TCAGGCGGTTGAGGCTGAAGATTATGATTCGGCGATGAGTAAGCTGGCTGAGCCTTTTCCTGATCTCGTCCTGCTTGACT
GGATGTTGCCTGGTGGTAGTGGCATCAACCTCATCAAGCATATGAAGCGCGAAGAGATGACGCGCAACATTCCTGTTGTG
ATGCTCACCGCCCGTGGTGAAGAAGAAGATAAAGTTCGCGGCTTAGAAGTGGGCGCGGATGATTACATTACCAAACCGTT
TTCACCTAAAGAGTTGGTTGCCCGTTTAAAAGCGGTGATCCGCCGTGTCACTCCGACGGCGTTGGAGGATGTGATTGATG
TTCAAGGTTTGAAGTTAGATCCGGTCTCACACCGTGTGACCGCTAACGATCAGCCGTTGGATATGGGACCGACTGAGTTC
AAGATGTTACACTTCTTCATGACACACCAAGAGCGTGTGTATAGCCGCGAGCAGCTACTCAATAATGTTTGGGGCACCAA
CGTGTATGTGGAAGATCGCACGGTAGACGTCCACATTCGTCGTCTACGTAAAGCCTTAGAAGACGCTGGACATGATAAGC
TGATCCAGACGGTGCGCGGCGCGGGGTATCGTTTTTCAACCAAAGCCTAA