Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000026  |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (320 a.a.); Nucleotide sequence (960 bp) |
| Response to T6SS attack | protective |
| Known or putative function | D-alanine--D-alanine ligase |
| Description | Tat-TseL was highly toxic to the ddlB (PA4410) mutant because no transformant could be obtained. |
| Reference | [1] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336 |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | PA4410 (ddlB) |
| Coordinates (Strand) | 4942677..4943636 (-) |
| NCBI ID | 15599606 |
| RefSeq | NC_002516 |
| Uniprot ID | DDLB_PSEAE |
| KEGG ID | pae:PA4410 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Dala_Dala_lig_C | PF07478.15 | 2.8e-57 | 120..311 |
| Dala_Dala_lig_N | PF01820.23 | 1.9e-10 | 68..102 |
| Dala_Dala_lig_N | PF01820.23 | 4.1e-07 | 20..57 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-319 | MNLCLDSLLNGTQDPKAFGRVAVLFGGKSAEREVSLKSGAMVLQSLLAAGVDAFGIDVGEDLLQRLVEEKIDRAFIILHGRGGEDGSMQGLLECAGIPYTGSGVLASALAMDKLRTKRVWLSLGLPTPDYAVLASEDDCREAAQRLGFPLIVKPAHEGSSIGMAKVGGLDELIAAWREAARYDSQVLVEQWISGPEFTVATLRGQVLPAIRLGTPHTFYDYDAKYLASDTRYQVPCGLDEAKERELKELTARACDALGIQGWGRADVMQDAEGRFWLLEVNTAPGMTDHSLVPMAARAAGLDFQQLVLAILADSREARG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9599 | - | - |
Protein sequence: 320 a.a. .
>RSF000026 NC_002516:c4943636-4942677 [Pseudomonas aeruginosa PAO1]
MNLCLDSLLNGTQDPKAFGRVAVLFGGKSAEREVSLKSGAMVLQSLLAAGVDAFGIDVGEDLLQRLVEEKIDRAFIILHG
RGGEDGSMQGLLECAGIPYTGSGVLASALAMDKLRTKRVWLSLGLPTPDYAVLASEDDCREAAQRLGFPLIVKPAHEGSS
IGMAKVGGLDELIAAWREAARYDSQVLVEQWISGPEFTVATLRGQVLPAIRLGTPHTFYDYDAKYLASDTRYQVPCGLDE
AKERELKELTARACDALGIQGWGRADVMQDAEGRFWLLEVNTAPGMTDHSLVPMAARAAGLDFQQLVLAILADSREARG*
MNLCLDSLLNGTQDPKAFGRVAVLFGGKSAEREVSLKSGAMVLQSLLAAGVDAFGIDVGEDLLQRLVEEKIDRAFIILHG
RGGEDGSMQGLLECAGIPYTGSGVLASALAMDKLRTKRVWLSLGLPTPDYAVLASEDDCREAAQRLGFPLIVKPAHEGSS
IGMAKVGGLDELIAAWREAARYDSQVLVEQWISGPEFTVATLRGQVLPAIRLGTPHTFYDYDAKYLASDTRYQVPCGLDE
AKERELKELTARACDALGIQGWGRADVMQDAEGRFWLLEVNTAPGMTDHSLVPMAARAAGLDFQQLVLAILADSREARG*
Nucleotide sequence: 960 bp .
>RSF000026 NC_002516:c4943636-4942677 [Pseudomonas aeruginosa PAO1]
ATGAACCTTTGCCTCGATAGCCTGCTGAACGGCACCCAGGATCCCAAGGCGTTCGGCCGTGTCGCCGTGCTGTTCGGCGG
CAAGAGCGCCGAGCGCGAGGTGTCGCTGAAGTCCGGCGCGATGGTCCTGCAATCCCTGCTGGCCGCCGGGGTCGATGCCT
TCGGCATCGATGTCGGCGAAGACCTGCTGCAACGCCTGGTCGAGGAGAAGATCGACCGTGCCTTCATCATTCTCCACGGT
CGTGGCGGCGAGGATGGCAGCATGCAGGGCCTGCTCGAGTGCGCGGGCATTCCCTACACCGGCAGCGGCGTGCTGGCCTC
GGCGCTGGCGATGGACAAGCTGCGGACCAAGCGGGTCTGGCTCAGCCTCGGCCTGCCGACCCCGGACTACGCGGTGCTGG
CCAGCGAGGATGACTGCCGCGAAGCGGCGCAGCGACTGGGTTTCCCGCTGATCGTCAAGCCGGCTCACGAAGGCTCGAGC
ATCGGCATGGCCAAGGTCGGCGGGCTCGACGAATTGATCGCGGCGTGGCGCGAAGCGGCCCGCTACGACTCGCAGGTGCT
GGTCGAGCAGTGGATCAGCGGCCCGGAATTCACCGTGGCGACCTTGCGCGGGCAGGTGCTGCCGGCGATCCGCCTGGGCA
CGCCGCACACCTTCTACGACTACGACGCCAAGTACCTGGCCAGCGATACCCGCTACCAGGTGCCCTGCGGTCTCGACGAG
GCCAAGGAACGCGAGCTGAAGGAACTCACCGCGCGCGCCTGCGACGCCCTGGGCATCCAGGGCTGGGGGCGGGCGGACGT
GATGCAGGACGCCGAAGGGCGTTTCTGGCTGCTTGAAGTCAACACCGCACCGGGCATGACCGACCACAGCCTGGTGCCTA
TGGCCGCGCGGGCCGCCGGCCTGGATTTCCAGCAACTGGTGCTGGCGATCCTGGCCGATAGCCGCGAGGCGAGGGGATAA
ATGAACCTTTGCCTCGATAGCCTGCTGAACGGCACCCAGGATCCCAAGGCGTTCGGCCGTGTCGCCGTGCTGTTCGGCGG
CAAGAGCGCCGAGCGCGAGGTGTCGCTGAAGTCCGGCGCGATGGTCCTGCAATCCCTGCTGGCCGCCGGGGTCGATGCCT
TCGGCATCGATGTCGGCGAAGACCTGCTGCAACGCCTGGTCGAGGAGAAGATCGACCGTGCCTTCATCATTCTCCACGGT
CGTGGCGGCGAGGATGGCAGCATGCAGGGCCTGCTCGAGTGCGCGGGCATTCCCTACACCGGCAGCGGCGTGCTGGCCTC
GGCGCTGGCGATGGACAAGCTGCGGACCAAGCGGGTCTGGCTCAGCCTCGGCCTGCCGACCCCGGACTACGCGGTGCTGG
CCAGCGAGGATGACTGCCGCGAAGCGGCGCAGCGACTGGGTTTCCCGCTGATCGTCAAGCCGGCTCACGAAGGCTCGAGC
ATCGGCATGGCCAAGGTCGGCGGGCTCGACGAATTGATCGCGGCGTGGCGCGAAGCGGCCCGCTACGACTCGCAGGTGCT
GGTCGAGCAGTGGATCAGCGGCCCGGAATTCACCGTGGCGACCTTGCGCGGGCAGGTGCTGCCGGCGATCCGCCTGGGCA
CGCCGCACACCTTCTACGACTACGACGCCAAGTACCTGGCCAGCGATACCCGCTACCAGGTGCCCTGCGGTCTCGACGAG
GCCAAGGAACGCGAGCTGAAGGAACTCACCGCGCGCGCCTGCGACGCCCTGGGCATCCAGGGCTGGGGGCGGGCGGACGT
GATGCAGGACGCCGAAGGGCGTTTCTGGCTGCTTGAAGTCAACACCGCACCGGGCATGACCGACCACAGCCTGGTGCCTA
TGGCCGCGCGGGCCGCCGGCCTGGATTTCCAGCAACTGGTGCTGGCGATCCTGGCCGATAGCCGCGAGGCGAGGGGATAA