Detailed information of Recipient susceptibility factor


Summary

ID RSF000026 experimental
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (320 a.a.); Nucleotide sequence (960 bp)
Response to T6SS attack protective
Known or putative function D-alanine--D-alanine ligase
Description Tat-TseL was highly toxic to the ddlB (PA4410) mutant because no transformant could be obtained.
Reference
experimental Experimental investigation has been performed.

External database links

Locus tag (Gene) PA4410 (ddlB)
Coordinates (Strand) 4942677..4943636 (-)
NCBI ID 15599606
RefSeq NC_002516
Uniprot ID DDLB_PSEAE
KEGG ID pae:PA4410
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Dala_Dala_lig_C PF07478.15 2.8e-57 120..311
Dala_Dala_lig_N PF01820.23 1.9e-10 68..102
Dala_Dala_lig_N PF01820.23 4.1e-07 20..57

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-319 MNLCLDSLLNGTQDPKAFGRVAVLFGGKSAEREVSLKSGAMVLQSLLAAGVDAFGIDVGEDLLQRLVEEKIDRAFIILHGRGGEDGSMQGLLECAGIPYTGSGVLASALAMDKLRTKRVWLSLGLPTPDYAVLASEDDCREAAQRLGFPLIVKPAHEGSSIGMAKVGGLDELIAAWREAARYDSQVLVEQWISGPEFTVATLRGQVLPAIRLGTPHTFYDYDAKYLASDTRYQVPCGLDEAKERELKELTARACDALGIQGWGRADVMQDAEGRFWLLEVNTAPGMTDHSLVPMAARAAGLDFQQLVLAILADSREARG



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9599 - -



  Protein sequence: 320 a.a.    .

>RSF000026 NC_002516:c4943636-4942677 [Pseudomonas aeruginosa PAO1]
MNLCLDSLLNGTQDPKAFGRVAVLFGGKSAEREVSLKSGAMVLQSLLAAGVDAFGIDVGEDLLQRLVEEKIDRAFIILHG
RGGEDGSMQGLLECAGIPYTGSGVLASALAMDKLRTKRVWLSLGLPTPDYAVLASEDDCREAAQRLGFPLIVKPAHEGSS
IGMAKVGGLDELIAAWREAARYDSQVLVEQWISGPEFTVATLRGQVLPAIRLGTPHTFYDYDAKYLASDTRYQVPCGLDE
AKERELKELTARACDALGIQGWGRADVMQDAEGRFWLLEVNTAPGMTDHSLVPMAARAAGLDFQQLVLAILADSREARG*

  Nucleotide sequence: 960 bp    .

>RSF000026 NC_002516:c4943636-4942677 [Pseudomonas aeruginosa PAO1]
ATGAACCTTTGCCTCGATAGCCTGCTGAACGGCACCCAGGATCCCAAGGCGTTCGGCCGTGTCGCCGTGCTGTTCGGCGG
CAAGAGCGCCGAGCGCGAGGTGTCGCTGAAGTCCGGCGCGATGGTCCTGCAATCCCTGCTGGCCGCCGGGGTCGATGCCT
TCGGCATCGATGTCGGCGAAGACCTGCTGCAACGCCTGGTCGAGGAGAAGATCGACCGTGCCTTCATCATTCTCCACGGT
CGTGGCGGCGAGGATGGCAGCATGCAGGGCCTGCTCGAGTGCGCGGGCATTCCCTACACCGGCAGCGGCGTGCTGGCCTC
GGCGCTGGCGATGGACAAGCTGCGGACCAAGCGGGTCTGGCTCAGCCTCGGCCTGCCGACCCCGGACTACGCGGTGCTGG
CCAGCGAGGATGACTGCCGCGAAGCGGCGCAGCGACTGGGTTTCCCGCTGATCGTCAAGCCGGCTCACGAAGGCTCGAGC
ATCGGCATGGCCAAGGTCGGCGGGCTCGACGAATTGATCGCGGCGTGGCGCGAAGCGGCCCGCTACGACTCGCAGGTGCT
GGTCGAGCAGTGGATCAGCGGCCCGGAATTCACCGTGGCGACCTTGCGCGGGCAGGTGCTGCCGGCGATCCGCCTGGGCA
CGCCGCACACCTTCTACGACTACGACGCCAAGTACCTGGCCAGCGATACCCGCTACCAGGTGCCCTGCGGTCTCGACGAG
GCCAAGGAACGCGAGCTGAAGGAACTCACCGCGCGCGCCTGCGACGCCCTGGGCATCCAGGGCTGGGGGCGGGCGGACGT
GATGCAGGACGCCGAAGGGCGTTTCTGGCTGCTTGAAGTCAACACCGCACCGGGCATGACCGACCACAGCCTGGTGCCTA
TGGCCGCGCGGGCCGCCGGCCTGGATTTCCAGCAACTGGTGCTGGCGATCCTGGCCGATAGCCGCGAGGCGAGGGGATAA