Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000025  |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (115 a.a.); Nucleotide sequence (345 bp) |
| Response to T6SS attack | protective |
| Known or putative function | a predicted osmotic stress resistance |
| Description | By expressing Tat-TseL in those mutants, we found ~100-fold increased toxicity in six mutants compared with wild-type P. aeruginosa. |
| Reference | [1] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336 |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | PA4739 (osmY) |
| Coordinates (Strand) | 5322757..5323101 (-) |
| NCBI ID | 15599933 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9HV60_PSEAE |
| KEGG ID | pae:PA4739 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| BON | PF04972.19 | 2.4e-18 | 45..108 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-114 | MKFSKLAIAAATATALSFSMANAFAAQPISLAANDTMQKTEEAVSDTWITSKVKSSLIANKNVSGVDIKVETNKGVVSLSGNVKSDAERDLAIETAKGIKGVKAVSADGLKSVE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9975 | pos: 25-26. AFA-AQ. | MKFSKLAIAAATATALSFSMANAFA |
Protein sequence: 115 a.a. .
>RSF000025 NC_002516:c5323101-5322757 [Pseudomonas aeruginosa PAO1]
MKFSKLAIAAATATALSFSMANAFAAQPISLAANDTMQKTEEAVSDTWITSKVKSSLIANKNVSGVDIKVETNKGVVSLS
GNVKSDAERDLAIETAKGIKGVKAVSADGLKSVE*
MKFSKLAIAAATATALSFSMANAFAAQPISLAANDTMQKTEEAVSDTWITSKVKSSLIANKNVSGVDIKVETNKGVVSLS
GNVKSDAERDLAIETAKGIKGVKAVSADGLKSVE*
Nucleotide sequence: 345 bp .
>RSF000025 NC_002516:c5323101-5322757 [Pseudomonas aeruginosa PAO1]
ATGAAATTCAGCAAACTCGCTATTGCCGCTGCTACTGCCACTGCTCTGAGCTTCTCGATGGCCAATGCGTTCGCTGCCCA
GCCCATCAGCCTGGCGGCCAACGACACTATGCAGAAAACCGAAGAAGCGGTTTCCGATACCTGGATCACCAGCAAGGTGA
AATCCAGCCTGATCGCCAACAAGAATGTCAGTGGCGTCGATATCAAGGTCGAGACCAACAAAGGTGTGGTTTCCCTTTCC
GGCAACGTGAAGAGCGATGCCGAGCGTGACCTGGCGATCGAGACCGCCAAGGGCATCAAGGGCGTGAAAGCCGTTTCCGC
CGATGGCCTGAAATCGGTCGAGTAA
ATGAAATTCAGCAAACTCGCTATTGCCGCTGCTACTGCCACTGCTCTGAGCTTCTCGATGGCCAATGCGTTCGCTGCCCA
GCCCATCAGCCTGGCGGCCAACGACACTATGCAGAAAACCGAAGAAGCGGTTTCCGATACCTGGATCACCAGCAAGGTGA
AATCCAGCCTGATCGCCAACAAGAATGTCAGTGGCGTCGATATCAAGGTCGAGACCAACAAAGGTGTGGTTTCCCTTTCC
GGCAACGTGAAGAGCGATGCCGAGCGTGACCTGGCGATCGAGACCGCCAAGGGCATCAAGGGCGTGAAAGCCGTTTCCGC
CGATGGCCTGAAATCGGTCGAGTAA