Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000024  |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (214 a.a.); Nucleotide sequence (642 bp) |
| Response to T6SS attack | protective |
| Known or putative function | a putative RcsB-like regulator |
| Description | By expressing Tat-TseL in those mutants, we found ~100-fold increased toxicity in six mutants compared with wild-type P. aeruginosa. |
| Reference | [1] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336 |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | PA3714 |
| Coordinates (Strand) | 4160329..4160970 (+) |
| NCBI ID | 15598909 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9HXS7_PSEAE |
| KEGG ID | pae:PA3714 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Response_reg | PF00072.26 | 5.1e-23 | 5..120 |
| GerE | PF00196.21 | 2.2e-10 | 152..204 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-213 | MKTRVILVDDHALTLIGMRYLLSAYDDLRIVAQAQDADGLLAQLEAHPCDLLITDLMMPGSQQADGLRLVQKVRRRYPDLPIIVVTMLGNPALVSSLLKLGIHGLVSKRGMLDDLPKAIRHAGRRPFISRSIAHLLEVGEAEHGKPLASLEQLTPREVEVLRLYGSGLAVGEIAIRLCRSKQTISAQKSSAMRKLGLDSNAGLFIYIRENGLA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9815 | - | - |
Protein sequence: 214 a.a. .
>RSF000024 NC_002516:4160329-4160970 [Pseudomonas aeruginosa PAO1]
MKTRVILVDDHALTLIGMRYLLSAYDDLRIVAQAQDADGLLAQLEAHPCDLLITDLMMPGSQQADGLRLVQKVRRRYPDL
PIIVVTMLGNPALVSSLLKLGIHGLVSKRGMLDDLPKAIRHAGRRPFISRSIAHLLEVGEAEHGKPLASLEQLTPREVEV
LRLYGSGLAVGEIAIRLCRSKQTISAQKSSAMRKLGLDSNAGLFIYIRENGLA*
MKTRVILVDDHALTLIGMRYLLSAYDDLRIVAQAQDADGLLAQLEAHPCDLLITDLMMPGSQQADGLRLVQKVRRRYPDL
PIIVVTMLGNPALVSSLLKLGIHGLVSKRGMLDDLPKAIRHAGRRPFISRSIAHLLEVGEAEHGKPLASLEQLTPREVEV
LRLYGSGLAVGEIAIRLCRSKQTISAQKSSAMRKLGLDSNAGLFIYIRENGLA*
Nucleotide sequence: 642 bp .
>RSF000024 NC_002516:4160329-4160970 [Pseudomonas aeruginosa PAO1]
ATGAAAACACGCGTCATCCTCGTCGACGACCACGCCCTCACCCTGATCGGCATGCGCTATCTGCTGAGTGCGTACGACGA
CCTGCGGATCGTCGCCCAGGCCCAGGACGCCGATGGCTTGCTGGCGCAGCTCGAAGCGCACCCCTGCGACCTGCTGATCA
CCGACCTGATGATGCCCGGCAGCCAGCAGGCCGACGGCTTGCGCCTGGTGCAGAAGGTCCGGCGGCGCTATCCGGACCTG
CCGATCATCGTCGTCACCATGCTCGGCAACCCGGCACTCGTTTCATCCCTGCTCAAGCTGGGCATTCACGGCCTGGTGAG
CAAACGCGGCATGCTCGACGATCTGCCCAAGGCGATCCGCCACGCCGGCCGTCGACCATTCATCTCGCGCTCCATCGCAC
ACCTGCTGGAAGTGGGCGAAGCCGAGCACGGCAAGCCGCTGGCCAGCCTCGAACAACTCACCCCGCGCGAAGTGGAAGTG
CTGCGCCTGTACGGCAGCGGCCTGGCGGTCGGGGAAATCGCCATCCGACTGTGCCGCAGCAAGCAGACCATCAGCGCGCA
AAAGAGCAGCGCCATGCGCAAGCTTGGCCTGGACAGCAATGCCGGACTCTTTATCTACATCCGGGAAAACGGCCTGGCCT
GA
ATGAAAACACGCGTCATCCTCGTCGACGACCACGCCCTCACCCTGATCGGCATGCGCTATCTGCTGAGTGCGTACGACGA
CCTGCGGATCGTCGCCCAGGCCCAGGACGCCGATGGCTTGCTGGCGCAGCTCGAAGCGCACCCCTGCGACCTGCTGATCA
CCGACCTGATGATGCCCGGCAGCCAGCAGGCCGACGGCTTGCGCCTGGTGCAGAAGGTCCGGCGGCGCTATCCGGACCTG
CCGATCATCGTCGTCACCATGCTCGGCAACCCGGCACTCGTTTCATCCCTGCTCAAGCTGGGCATTCACGGCCTGGTGAG
CAAACGCGGCATGCTCGACGATCTGCCCAAGGCGATCCGCCACGCCGGCCGTCGACCATTCATCTCGCGCTCCATCGCAC
ACCTGCTGGAAGTGGGCGAAGCCGAGCACGGCAAGCCGCTGGCCAGCCTCGAACAACTCACCCCGCGCGAAGTGGAAGTG
CTGCGCCTGTACGGCAGCGGCCTGGCGGTCGGGGAAATCGCCATCCGACTGTGCCGCAGCAAGCAGACCATCAGCGCGCA
AAAGAGCAGCGCCATGCGCAAGCTTGGCCTGGACAGCAATGCCGGACTCTTTATCTACATCCGGGAAAACGGCCTGGCCT
GA