Detailed information of Recipient susceptibility factor


Summary

ID RSF000023 experimental
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (787 a.a.); Nucleotide sequence (2361 bp)
Response to T6SS attack protective
Known or putative function a biofilm sensor/regulator
Description By expressing Tat-TseL in those mutants, we found ~100-fold increased toxicity in six mutants compared with wild-type P. aeruginosa.
Reference
experimental Experimental investigation has been performed.
External database links

Locus tag (Gene) PA2824 (sagS)
Coordinates (Strand) 3177090..3179450 (+)
NCBI ID 15598020
RefSeq NC_002516
Uniprot ID Q9I019_PSEAE
KEGG ID pae:PA2824
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
HATPase_c PF02518.28 1.2e-28 416..526
Response_reg PF00072.26 2e-27 665..776
HisKA PF00512.27 8.2e-18 306..370
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-26 MLGGRTSPRLIPAPMDIALTHRLSFK
Transmembrane helix 27-49 QASLTVLVAFILGTLLSLIQVGV
Inside 50-174 DYASQDASINREVRALLDVSHNPAARIAYNIDAELAQELVLGLLRSPAVVRAEIIDTSGLPLASASREPAESRLRPLSDFLFGHKRVYEDPLHVDHAPGEALGVLHLEIDTFVFGNDFLRRAGIT
Transmembrane helix 175-197 LLSGFVRSLLLSLILLVLFYTLL
Outside 198-786 TKPLVSLIQALSGHDPRSPARMRLPCPKGHERDEIGVLVEVINRQLGRISVEIEQRREAENRLTQYLEELESIVAARTAELKAANARLTLSNQELEEARQTALDMAQARASFLANMSHEIRTPLNGLLGMLSLSLDGPLTPEQRQQLSIAHDSGKVLVELLNDVLDLSKFEAGQLVLEQIPFDLGVLVEDTASLLSQNAAAGVELTCLVDPALPAQVSGDPTRIRQIVSNLLSNALKFTRLGRVDVRVEATAEGVRISVRDTGIGIAQEALDRIFQPFTQADAGITRQYGGTGLGLALTRKLCEAMQGELTVESTVGLGSLFSVGLPLAPVSPPLQALPLRGRVIAQCSANSGLAQLLQTWLPRWGLEYKRLETDDSLLGHSLDVLISDCPDCLMGLRPSIGTPILLVTAYGSFLEPELARRLSPLRQLARPLSRNQLYQALKHVLEHTAIAPSSASDTTGEQRNTRVLLVEDNPVNQLVAKGLLHKLGCQVWIAEHGLNALKMLEEHPIDLVLMDCNMPVMDGYEATRQIRDSGRWGGLPIIALTANALPDERERCRAAGMDDYLAKPFHRDELKAILDRWCPLTASD


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9892 - -


  Protein sequence: 787 a.a.    .

>RSF000023 NC_002516:3177090-3179450 [Pseudomonas aeruginosa PAO1]
MLGGRTSPRLIPAPMDIALTHRLSFKQASLTVLVAFILGTLLSLIQVGVDYASQDASINREVRALLDVSHNPAARIAYNI
DAELAQELVLGLLRSPAVVRAEIIDTSGLPLASASREPAESRLRPLSDFLFGHKRVYEDPLHVDHAPGEALGVLHLEIDT
FVFGNDFLRRAGITLLSGFVRSLLLSLILLVLFYTLLTKPLVSLIQALSGHDPRSPARMRLPCPKGHERDEIGVLVEVIN
RQLGRISVEIEQRREAENRLTQYLEELESIVAARTAELKAANARLTLSNQELEEARQTALDMAQARASFLANMSHEIRTP
LNGLLGMLSLSLDGPLTPEQRQQLSIAHDSGKVLVELLNDVLDLSKFEAGQLVLEQIPFDLGVLVEDTASLLSQNAAAGV
ELTCLVDPALPAQVSGDPTRIRQIVSNLLSNALKFTRLGRVDVRVEATAEGVRISVRDTGIGIAQEALDRIFQPFTQADA
GITRQYGGTGLGLALTRKLCEAMQGELTVESTVGLGSLFSVGLPLAPVSPPLQALPLRGRVIAQCSANSGLAQLLQTWLP
RWGLEYKRLETDDSLLGHSLDVLISDCPDCLMGLRPSIGTPILLVTAYGSFLEPELARRLSPLRQLARPLSRNQLYQALK
HVLEHTAIAPSSASDTTGEQRNTRVLLVEDNPVNQLVAKGLLHKLGCQVWIAEHGLNALKMLEEHPIDLVLMDCNMPVMD
GYEATRQIRDSGRWGGLPIIALTANALPDERERCRAAGMDDYLAKPFHRDELKAILDRWCPLTASD*

  Nucleotide sequence: 2361 bp    .

>RSF000023 NC_002516:3177090-3179450 [Pseudomonas aeruginosa PAO1]
ATGCTAGGCGGCAGAACCTCGCCAAGGCTGATCCCCGCCCCCATGGATATAGCGCTGACCCATCGCCTGTCGTTCAAACA
GGCCAGCCTCACCGTGCTGGTGGCCTTTATCCTGGGAACCCTGCTCAGCCTGATTCAGGTTGGCGTCGATTATGCCAGCC
AGGACGCATCCATCAATCGCGAAGTGCGCGCGCTGCTGGACGTCAGCCACAACCCCGCCGCGCGAATCGCCTACAACATC
GATGCGGAGCTGGCCCAGGAGCTGGTCCTCGGCCTGCTGCGCTCGCCCGCCGTGGTCCGCGCGGAAATCATCGACACCTC
CGGCCTGCCGCTGGCCAGCGCCTCGCGCGAACCCGCCGAAAGCCGCCTGCGGCCGCTCAGCGACTTCCTCTTCGGCCACA
AGCGGGTCTACGAGGACCCGTTGCATGTCGACCATGCGCCGGGCGAGGCCCTCGGCGTACTGCACCTGGAAATCGACACC
TTCGTGTTCGGCAACGACTTCCTCCGTCGCGCCGGCATCACCCTGCTCTCCGGCTTCGTGCGCAGCCTGCTGCTGTCGCT
GATCCTGCTGGTGCTCTTCTATACCCTGCTGACCAAGCCCCTGGTCAGCCTGATCCAGGCCCTCAGCGGACACGATCCAC
GCTCGCCGGCACGCATGCGCCTGCCCTGCCCCAAGGGCCACGAACGCGACGAGATCGGCGTGCTGGTGGAAGTCATCAAC
CGCCAGCTCGGGCGCATCTCGGTAGAGATCGAACAGCGCCGGGAGGCGGAGAACCGCCTGACCCAGTACCTGGAGGAGCT
GGAAAGCATCGTCGCCGCCCGTACCGCCGAGCTGAAGGCCGCCAACGCGCGCCTGACGCTATCCAACCAGGAACTCGAAG
AAGCCCGCCAGACCGCTCTCGATATGGCCCAGGCCCGCGCCAGCTTCCTGGCCAACATGAGCCACGAGATCCGCACGCCG
CTGAACGGCCTGCTCGGCATGCTCTCGCTGAGCCTCGACGGCCCGCTCACCCCGGAACAGCGCCAGCAACTGTCGATCGC
CCACGACTCCGGCAAGGTGCTGGTGGAATTGCTCAACGACGTGCTCGACCTCTCCAAGTTCGAGGCCGGGCAACTGGTCC
TGGAACAGATCCCGTTCGACCTGGGCGTCCTGGTGGAAGACACCGCCAGCCTCCTCTCGCAGAACGCCGCGGCCGGCGTG
GAGCTGACCTGCCTGGTCGACCCCGCCCTGCCCGCCCAGGTTTCCGGCGACCCGACGCGGATTCGCCAGATCGTCAGCAA
CCTGCTGTCCAACGCGCTGAAGTTCACCCGCCTGGGGCGGGTCGACGTTCGCGTGGAAGCCACCGCCGAGGGTGTGCGCA
TCAGTGTGCGCGACACTGGCATCGGTATCGCCCAGGAGGCGCTGGATCGCATCTTCCAACCGTTCACCCAGGCCGACGCG
GGTATCACCCGCCAATACGGCGGCACTGGCCTCGGCCTGGCCCTCACGCGCAAGCTGTGCGAGGCCATGCAGGGCGAGCT
GACGGTGGAGTCGACGGTCGGCCTGGGCAGCCTGTTCAGCGTCGGCCTGCCGCTGGCGCCGGTCAGCCCTCCGCTGCAGG
CGCTGCCGCTGCGCGGCCGGGTGATCGCGCAATGCTCGGCCAACAGCGGGCTGGCGCAGCTGCTGCAAACCTGGCTGCCG
CGCTGGGGCCTGGAGTACAAGCGTCTGGAGACCGACGACAGCCTGCTCGGCCACTCGCTCGACGTACTGATATCGGATTG
CCCGGACTGTCTGATGGGACTGCGTCCGAGCATCGGTACGCCGATCCTGCTGGTCACCGCCTACGGCAGTTTCCTCGAAC
CGGAGCTGGCCCGCCGCCTGTCGCCGCTGCGCCAACTGGCCAGGCCGCTGAGTCGCAACCAGTTGTACCAGGCCCTGAAA
CACGTGCTCGAACACACTGCCATAGCCCCGTCGAGCGCCTCGGACACCACGGGAGAACAGCGCAACACCCGGGTCCTGCT
GGTGGAGGACAACCCGGTCAACCAGTTGGTCGCCAAGGGCCTGCTGCACAAGCTCGGCTGCCAGGTATGGATCGCCGAAC
ACGGGCTGAATGCCCTGAAGATGCTGGAGGAGCATCCCATCGACCTGGTCCTGATGGACTGCAACATGCCAGTGATGGAC
GGCTACGAAGCGACCCGGCAGATCCGCGACAGCGGACGCTGGGGCGGCCTGCCGATCATCGCGCTGACCGCCAACGCCCT
GCCGGACGAGCGCGAACGTTGTCGTGCCGCCGGCATGGACGACTACCTGGCCAAGCCTTTCCACCGCGATGAACTGAAAG
CCATCCTCGACCGTTGGTGCCCGCTCACCGCGAGCGACTAG