Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000022  |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (128 a.a.); Nucleotide sequence (384 bp) |
| Response to T6SS attack | protective |
| Known or putative function | a membrane-bound lysozyme inhibitor |
| Description | By expressing Tat-TseL in those mutants, we found ~100-fold increased toxicity in six mutants compared with wild-type P. aeruginosa. |
| Reference | [1] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336 |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | PA0867 (mliC) |
| Coordinates (Strand) | 948776..949159 (+) |
| NCBI ID | 15596064 |
| RefSeq | NC_002516 |
| Uniprot ID | MLIC_PSEAE |
| KEGG ID | pae:PA0867 |
| PDB ID | 3F6Z |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| MliC | PF09864.11 | 2.3e-21 | 49..114 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-127 | MKKALWLLLAAVPVVLVACGGSDDDKQTAQVDYLALPGDAKLDTRSVDYKCENGRKFTVQYLNKGDNSLAVVPVSDNSTLVFSNVISASGAKYAAGQYIWWTKGEEATLYGDWKGGEPTDGVACKER |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9976 | pos: 18-19. LVA-CG. | MKKALWLLLAAVPVVLVA |
Protein sequence: 128 a.a. .
>RSF000022 NC_002516:948776-949159 [Pseudomonas aeruginosa PAO1]
MKKALWLLLAAVPVVLVACGGSDDDKQTAQVDYLALPGDAKLDTRSVDYKCENGRKFTVQYLNKGDNSLAVVPVSDNSTL
VFSNVISASGAKYAAGQYIWWTKGEEATLYGDWKGGEPTDGVACKER*
MKKALWLLLAAVPVVLVACGGSDDDKQTAQVDYLALPGDAKLDTRSVDYKCENGRKFTVQYLNKGDNSLAVVPVSDNSTL
VFSNVISASGAKYAAGQYIWWTKGEEATLYGDWKGGEPTDGVACKER*
Nucleotide sequence: 384 bp .
>RSF000022 NC_002516:948776-949159 [Pseudomonas aeruginosa PAO1]
ATGAAAAAAGCGCTCTGGCTGCTTCTGGCTGCCGTTCCCGTGGTCCTGGTCGCCTGCGGCGGCAGCGACGACGACAAGCA
GACCGCCCAGGTCGATTACCTGGCCCTGCCGGGGGATGCCAAGCTGGATACCCGCAGCGTCGACTACAAGTGCGAGAACG
GTCGCAAGTTCACCGTGCAGTACCTGAACAAGGGCGACAACAGCCTCGCCGTGGTGCCGGTCAGCGACAACTCGACGCTG
GTCTTCAGCAATGTGATTTCCGCCTCCGGCGCCAAGTACGCCGCCGGCCAGTACATCTGGTGGACCAAGGGCGAGGAAGC
CACCCTCTACGGCGACTGGAAGGGCGGCGAGCCCACCGACGGGGTCGCCTGCAAGGAGCGTTGA
ATGAAAAAAGCGCTCTGGCTGCTTCTGGCTGCCGTTCCCGTGGTCCTGGTCGCCTGCGGCGGCAGCGACGACGACAAGCA
GACCGCCCAGGTCGATTACCTGGCCCTGCCGGGGGATGCCAAGCTGGATACCCGCAGCGTCGACTACAAGTGCGAGAACG
GTCGCAAGTTCACCGTGCAGTACCTGAACAAGGGCGACAACAGCCTCGCCGTGGTGCCGGTCAGCGACAACTCGACGCTG
GTCTTCAGCAATGTGATTTCCGCCTCCGGCGCCAAGTACGCCGCCGGCCAGTACATCTGGTGGACCAAGGGCGAGGAAGC
CACCCTCTACGGCGACTGGAAGGGCGGCGAGCCCACCGACGGGGTCGCCTGCAAGGAGCGTTGA