Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000021  |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (466 a.a.); Nucleotide sequence (1398 bp) |
| Response to T6SS attack | protective |
| Known or putative function | a putative amino acid permease PA4804 |
| Description | By expressing Tat-TseL in those mutants, we found ~100-fold increased toxicity in six mutants compared with wild-type P. aeruginosa. |
| Reference | [1] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336 |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | PA4804 |
| Coordinates (Strand) | 5388702..5390099 (-) |
| NCBI ID | 15599998 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9HV05_PSEAE |
| KEGG ID | pae:PA4804 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| AA_permease_2 | PF13520.8 | 2.6e-51 | 18..435 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-12 | MDDNKNKVLRLR |
| Transmembrane helix | 13-30 | DVVLYTVSAMLFMDQIAL |
| Outside | 31-39 | ASSLGPSSL |
| Transmembrane helix | 40-62 | FWWLYVLVLLFLPMAMMTSELGT |
| Inside | 63-89 | AFPANGGVYHWVRSAFGFRWGARVSWM |
| Transmembrane helix | 90-112 | YWVNNALWMPSVYTLFGSMLGAF |
| Outside | 113-116 | YFPE |
| Transmembrane helix | 117-139 | LSLWGKIAIGIALALLTAAFNVV |
| Inside | 140-151 | ALRLGKWLPNLG |
| Transmembrane helix | 152-171 | ALLKLLAVLALGVGGLHFGW |
| Outside | 172-190 | NHGFANDFSLDSIVPSSPG |
| Transmembrane helix | 191-213 | QMAALGVMVYGIMGTELACCSAA |
| Inside | 214-225 | EMRNARRDIPRA |
| Transmembrane helix | 226-248 | VLISGLIVGAFNIFGTLGVLAAV |
| Outside | 249-270 | PAEETDVTRIFAHTLYNIYGHD |
| Transmembrane helix | 271-293 | GAGGMLADLVGAFVLFTLFTNMV |
| Inside | 294-325 | TWSMGTNRAAVEAAKAGELPALFGVVHSRHGT |
| Transmembrane helix | 326-348 | PIGSAVLASAVSIVLLLLYGLVA |
| Outside | 349-357 | HTAEELFWT |
| Transmembrane helix | 358-380 | LLSIFAMVFMMPYVLMCLAFVRL |
| Inside | 381-400 | RRADPRPRPYRMPLGDRLAS |
| Transmembrane helix | 401-423 | LWALFVALHVLAGICLFVVTPGA |
| Outside | 424-426 | PMD |
| Transmembrane helix | 427-449 | WAYAGKIVGGVALALAVGELLIR |
| Inside | 450-465 | QAARRRGVMSLRGAYG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9855 | - | - |
Protein sequence: 466 a.a. .
>RSF000021 NC_002516:c5390099-5388702 [Pseudomonas aeruginosa PAO1]
MDDNKNKVLRLRDVVLYTVSAMLFMDQIALASSLGPSSLFWWLYVLVLLFLPMAMMTSELGTAFPANGGVYHWVRSAFGF
RWGARVSWMYWVNNALWMPSVYTLFGSMLGAFYFPELSLWGKIAIGIALALLTAAFNVVALRLGKWLPNLGALLKLLAVL
ALGVGGLHFGWNHGFANDFSLDSIVPSSPGQMAALGVMVYGIMGTELACCSAAEMRNARRDIPRAVLISGLIVGAFNIFG
TLGVLAAVPAEETDVTRIFAHTLYNIYGHDGAGGMLADLVGAFVLFTLFTNMVTWSMGTNRAAVEAAKAGELPALFGVVH
SRHGTPIGSAVLASAVSIVLLLLYGLVAHTAEELFWTLLSIFAMVFMMPYVLMCLAFVRLRRADPRPRPYRMPLGDRLAS
LWALFVALHVLAGICLFVVTPGAPMDWAYAGKIVGGVALALAVGELLIRQAARRRGVMSLRGAYG*
MDDNKNKVLRLRDVVLYTVSAMLFMDQIALASSLGPSSLFWWLYVLVLLFLPMAMMTSELGTAFPANGGVYHWVRSAFGF
RWGARVSWMYWVNNALWMPSVYTLFGSMLGAFYFPELSLWGKIAIGIALALLTAAFNVVALRLGKWLPNLGALLKLLAVL
ALGVGGLHFGWNHGFANDFSLDSIVPSSPGQMAALGVMVYGIMGTELACCSAAEMRNARRDIPRAVLISGLIVGAFNIFG
TLGVLAAVPAEETDVTRIFAHTLYNIYGHDGAGGMLADLVGAFVLFTLFTNMVTWSMGTNRAAVEAAKAGELPALFGVVH
SRHGTPIGSAVLASAVSIVLLLLYGLVAHTAEELFWTLLSIFAMVFMMPYVLMCLAFVRLRRADPRPRPYRMPLGDRLAS
LWALFVALHVLAGICLFVVTPGAPMDWAYAGKIVGGVALALAVGELLIRQAARRRGVMSLRGAYG*
Nucleotide sequence: 1398 bp .
>RSF000021 NC_002516:c5390099-5388702 [Pseudomonas aeruginosa PAO1]
ATGGACGACAACAAGAACAAGGTCCTGCGGTTGCGCGATGTGGTGCTCTACACGGTCTCGGCGATGCTCTTCATGGACCA
GATCGCCCTGGCTTCCTCCCTCGGTCCGTCGAGCCTGTTCTGGTGGCTCTACGTGCTGGTCCTGCTGTTCCTGCCGATGG
CCATGATGACCTCCGAGCTGGGCACCGCCTTCCCCGCCAACGGCGGCGTCTACCACTGGGTGCGCAGCGCCTTCGGCTTC
CGCTGGGGGGCCCGGGTGTCATGGATGTACTGGGTCAACAACGCACTGTGGATGCCTTCGGTCTACACCCTGTTCGGCAG
CATGCTCGGCGCCTTCTATTTCCCCGAGCTGAGCCTCTGGGGCAAGATCGCCATCGGCATCGCCCTGGCGCTGCTCACCG
CGGCGTTCAACGTGGTCGCGCTGCGCCTGGGCAAGTGGCTGCCGAACCTCGGCGCGCTGCTCAAGCTGCTCGCGGTGCTC
GCCCTGGGCGTCGGCGGCCTGCACTTCGGCTGGAACCATGGCTTCGCCAACGACTTCAGCCTGGACTCCATCGTCCCGTC
CTCGCCGGGCCAGATGGCCGCGCTGGGAGTGATGGTCTATGGCATCATGGGGACCGAGCTGGCCTGTTGCAGCGCCGCGG
AGATGCGCAACGCGCGCCGCGACATCCCGCGCGCGGTGCTGATCTCCGGGCTGATCGTCGGCGCCTTCAACATCTTCGGC
ACCCTCGGCGTGCTGGCCGCGGTACCGGCCGAGGAAACCGACGTCACCCGGATCTTCGCCCATACCCTCTACAACATCTA
CGGCCACGACGGCGCCGGCGGGATGCTCGCCGACCTGGTCGGCGCCTTCGTCCTGTTCACCCTCTTCACCAACATGGTCA
CCTGGAGCATGGGCACCAACCGTGCCGCGGTGGAAGCGGCCAAGGCCGGCGAGCTGCCGGCGCTGTTCGGCGTGGTCCAT
TCGCGCCACGGCACGCCGATCGGCTCGGCGGTGCTGGCCTCGGCGGTGAGCATCGTCCTGCTGCTGCTCTACGGCCTGGT
GGCGCACACTGCCGAGGAACTGTTCTGGACCCTGCTGTCGATCTTCGCCATGGTTTTCATGATGCCCTATGTGCTGATGT
GCCTGGCCTTCGTCCGCCTGCGCCGCGCCGACCCGCGTCCACGGCCCTACCGGATGCCGCTGGGCGACCGCCTGGCCAGT
CTCTGGGCGCTGTTCGTCGCGCTGCATGTGCTGGCCGGCATCTGCCTGTTCGTCGTCACCCCCGGCGCGCCGATGGACTG
GGCCTACGCCGGCAAGATCGTCGGCGGCGTGGCGCTGGCCCTGGCCGTGGGCGAACTGCTGATCCGCCAGGCGGCGCGAA
GGCGCGGCGTGATGTCACTGCGCGGTGCCTACGGTTGA
ATGGACGACAACAAGAACAAGGTCCTGCGGTTGCGCGATGTGGTGCTCTACACGGTCTCGGCGATGCTCTTCATGGACCA
GATCGCCCTGGCTTCCTCCCTCGGTCCGTCGAGCCTGTTCTGGTGGCTCTACGTGCTGGTCCTGCTGTTCCTGCCGATGG
CCATGATGACCTCCGAGCTGGGCACCGCCTTCCCCGCCAACGGCGGCGTCTACCACTGGGTGCGCAGCGCCTTCGGCTTC
CGCTGGGGGGCCCGGGTGTCATGGATGTACTGGGTCAACAACGCACTGTGGATGCCTTCGGTCTACACCCTGTTCGGCAG
CATGCTCGGCGCCTTCTATTTCCCCGAGCTGAGCCTCTGGGGCAAGATCGCCATCGGCATCGCCCTGGCGCTGCTCACCG
CGGCGTTCAACGTGGTCGCGCTGCGCCTGGGCAAGTGGCTGCCGAACCTCGGCGCGCTGCTCAAGCTGCTCGCGGTGCTC
GCCCTGGGCGTCGGCGGCCTGCACTTCGGCTGGAACCATGGCTTCGCCAACGACTTCAGCCTGGACTCCATCGTCCCGTC
CTCGCCGGGCCAGATGGCCGCGCTGGGAGTGATGGTCTATGGCATCATGGGGACCGAGCTGGCCTGTTGCAGCGCCGCGG
AGATGCGCAACGCGCGCCGCGACATCCCGCGCGCGGTGCTGATCTCCGGGCTGATCGTCGGCGCCTTCAACATCTTCGGC
ACCCTCGGCGTGCTGGCCGCGGTACCGGCCGAGGAAACCGACGTCACCCGGATCTTCGCCCATACCCTCTACAACATCTA
CGGCCACGACGGCGCCGGCGGGATGCTCGCCGACCTGGTCGGCGCCTTCGTCCTGTTCACCCTCTTCACCAACATGGTCA
CCTGGAGCATGGGCACCAACCGTGCCGCGGTGGAAGCGGCCAAGGCCGGCGAGCTGCCGGCGCTGTTCGGCGTGGTCCAT
TCGCGCCACGGCACGCCGATCGGCTCGGCGGTGCTGGCCTCGGCGGTGAGCATCGTCCTGCTGCTGCTCTACGGCCTGGT
GGCGCACACTGCCGAGGAACTGTTCTGGACCCTGCTGTCGATCTTCGCCATGGTTTTCATGATGCCCTATGTGCTGATGT
GCCTGGCCTTCGTCCGCCTGCGCCGCGCCGACCCGCGTCCACGGCCCTACCGGATGCCGCTGGGCGACCGCCTGGCCAGT
CTCTGGGCGCTGTTCGTCGCGCTGCATGTGCTGGCCGGCATCTGCCTGTTCGTCGTCACCCCCGGCGCGCCGATGGACTG
GGCCTACGCCGGCAAGATCGTCGGCGGCGTGGCGCTGGCCCTGGCCGTGGGCGAACTGCTGATCCGCCAGGCGGCGCGAA
GGCGCGGCGTGATGTCACTGCGCGGTGCCTACGGTTGA