Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000020  |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (516 a.a.); Nucleotide sequence (1548 bp) |
| Response to T6SS attack | protective |
| Known or putative function | amino acid biosynthesis |
| Description | By expressing Tat-TseL in those mutants, we found ~100-fold increased toxicity in six mutants compared with wild-type P. aeruginosa. |
| Reference | [1] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336 |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | PA1326 (ilvA2) |
| Coordinates (Strand) | 1437067..1438614 (+) |
| NCBI ID | 15596523 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9I418_PSEAE |
| KEGG ID | pae:PA1326 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| PALP | PF00291.27 | 1.2e-81 | 30..317 |
| Thr_dehydrat_C | PF00585.20 | 1.4e-25 | 430..513 |
| Thr_dehydrat_C | PF00585.20 | 3e-22 | 330..420 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-515 | MSASTLSASKPLLAGYVESILAAPVYDVAVETPLQVAPQLSQRLGNRVLLKREDLQPVFSFKIRGAYTRVARLSDEQKARGVITASAGNHAQGLALAAQRLGVRAVIVMPRTTPELKVKGVLARGGEALLHGDAFPDALAHALQLAEREGMTFVPPYDDPDVIAGQGTVAMEILRQHSGRLDAIFVPVGGGSLIAGIAAYVKHLRPDIRVIGVEPEDSNCLQAALAAGERVVLGQVGLFADGVAVAQIGACNFEVCKDHVDEVITVGSDEICAAIKDIYDDTRSITEPAGALAVAGIKKYVARERTEGQTLVAIDSGANINFDRLRHVAERAELGEQREAIIAVTVAERPGSFKAFCAALGRRQITEFNYRYHSDRQAHLFVGVQTHPLTDSRADLLAGLREQGFPVLDLTDNEMAKLHIRHMVGGHGTEVRRERLFRFEFPERPGALLNFLDKLGSRWNISLFHYRNHGAADGRVLAGLQVPDEERGELEAALQAIGYPYWEETHNPAYRLFAG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.8588 | - | - |
Protein sequence: 516 a.a. .
>RSF000020 NC_002516:1437067-1438614 [Pseudomonas aeruginosa PAO1]
MSASTLSASKPLLAGYVESILAAPVYDVAVETPLQVAPQLSQRLGNRVLLKREDLQPVFSFKIRGAYTRVARLSDEQKAR
GVITASAGNHAQGLALAAQRLGVRAVIVMPRTTPELKVKGVLARGGEALLHGDAFPDALAHALQLAEREGMTFVPPYDDP
DVIAGQGTVAMEILRQHSGRLDAIFVPVGGGSLIAGIAAYVKHLRPDIRVIGVEPEDSNCLQAALAAGERVVLGQVGLFA
DGVAVAQIGACNFEVCKDHVDEVITVGSDEICAAIKDIYDDTRSITEPAGALAVAGIKKYVARERTEGQTLVAIDSGANI
NFDRLRHVAERAELGEQREAIIAVTVAERPGSFKAFCAALGRRQITEFNYRYHSDRQAHLFVGVQTHPLTDSRADLLAGL
REQGFPVLDLTDNEMAKLHIRHMVGGHGTEVRRERLFRFEFPERPGALLNFLDKLGSRWNISLFHYRNHGAADGRVLAGL
QVPDEERGELEAALQAIGYPYWEETHNPAYRLFAG*
MSASTLSASKPLLAGYVESILAAPVYDVAVETPLQVAPQLSQRLGNRVLLKREDLQPVFSFKIRGAYTRVARLSDEQKAR
GVITASAGNHAQGLALAAQRLGVRAVIVMPRTTPELKVKGVLARGGEALLHGDAFPDALAHALQLAEREGMTFVPPYDDP
DVIAGQGTVAMEILRQHSGRLDAIFVPVGGGSLIAGIAAYVKHLRPDIRVIGVEPEDSNCLQAALAAGERVVLGQVGLFA
DGVAVAQIGACNFEVCKDHVDEVITVGSDEICAAIKDIYDDTRSITEPAGALAVAGIKKYVARERTEGQTLVAIDSGANI
NFDRLRHVAERAELGEQREAIIAVTVAERPGSFKAFCAALGRRQITEFNYRYHSDRQAHLFVGVQTHPLTDSRADLLAGL
REQGFPVLDLTDNEMAKLHIRHMVGGHGTEVRRERLFRFEFPERPGALLNFLDKLGSRWNISLFHYRNHGAADGRVLAGL
QVPDEERGELEAALQAIGYPYWEETHNPAYRLFAG*
Nucleotide sequence: 1548 bp .
>RSF000020 NC_002516:1437067-1438614 [Pseudomonas aeruginosa PAO1]
ATGAGCGCCTCGACCCTCAGCGCAAGCAAACCCCTGCTGGCCGGCTACGTGGAAAGCATCCTGGCCGCGCCGGTGTACGA
CGTCGCCGTGGAAACCCCCTTGCAGGTCGCGCCGCAGTTGTCCCAGCGCCTGGGCAACCGGGTGCTGCTCAAGCGCGAGG
ACCTGCAGCCGGTGTTCTCCTTCAAGATCCGCGGCGCCTACACCCGTGTCGCGCGCCTGAGCGACGAGCAGAAGGCCCGC
GGGGTGATCACCGCCTCCGCCGGCAACCATGCCCAGGGCCTGGCCCTGGCGGCGCAGCGCCTGGGCGTGCGGGCGGTGAT
CGTGATGCCGCGCACCACTCCCGAACTCAAGGTCAAGGGCGTGCTGGCACGCGGCGGCGAGGCGTTGCTGCACGGCGACG
CCTTCCCCGATGCGCTGGCCCATGCCCTGCAACTGGCCGAGCGCGAAGGCATGACCTTCGTCCCACCCTACGACGATCCG
GACGTGATCGCCGGACAGGGCACGGTGGCGATGGAGATCCTCCGCCAGCACAGCGGCCGCCTCGACGCGATCTTCGTGCC
GGTCGGCGGCGGCAGCCTGATCGCCGGGATCGCCGCCTACGTCAAGCACCTGCGTCCGGATATTCGCGTGATCGGCGTCG
AGCCGGAGGATTCCAACTGCCTGCAGGCGGCGCTGGCCGCCGGCGAACGGGTGGTGCTGGGCCAGGTCGGGCTGTTCGCC
GACGGGGTGGCGGTGGCGCAGATCGGCGCTTGCAACTTCGAGGTCTGCAAGGACCATGTCGACGAGGTGATCACCGTCGG
CAGCGACGAAATCTGCGCGGCGATCAAGGACATCTACGACGACACCCGTTCGATCACCGAGCCGGCCGGCGCCCTGGCCG
TAGCGGGGATCAAGAAGTACGTGGCCCGCGAACGCACCGAGGGGCAGACCCTGGTGGCCATCGACTCCGGTGCCAACATC
AACTTCGACCGCCTGCGCCACGTTGCCGAGCGCGCCGAACTGGGCGAACAGCGCGAAGCGATCATCGCCGTCACCGTGGC
CGAGCGGCCGGGCAGCTTCAAGGCCTTCTGCGCGGCGCTGGGCCGGCGCCAGATCACCGAGTTCAACTACCGCTACCACA
GCGACCGCCAGGCCCACCTGTTCGTCGGCGTGCAGACCCATCCGCTCACCGACAGCCGCGCCGACCTGCTCGCCGGCCTG
CGCGAGCAGGGCTTCCCGGTACTCGACCTGACCGACAACGAGATGGCCAAGCTGCACATCCGCCACATGGTCGGCGGCCA
CGGTACCGAGGTGCGCCGCGAGCGGCTGTTCCGCTTCGAGTTTCCCGAACGCCCCGGGGCCTTGCTGAACTTCCTCGACA
AGCTCGGCTCGCGCTGGAACATCAGCCTGTTCCACTACCGCAACCACGGCGCCGCCGACGGTCGGGTGCTGGCCGGATTG
CAGGTGCCGGACGAGGAGCGCGGCGAGCTGGAGGCGGCGTTGCAAGCCATCGGCTATCCGTACTGGGAGGAAACCCACAA
CCCGGCCTACCGGCTGTTCGCCGGTTGA
ATGAGCGCCTCGACCCTCAGCGCAAGCAAACCCCTGCTGGCCGGCTACGTGGAAAGCATCCTGGCCGCGCCGGTGTACGA
CGTCGCCGTGGAAACCCCCTTGCAGGTCGCGCCGCAGTTGTCCCAGCGCCTGGGCAACCGGGTGCTGCTCAAGCGCGAGG
ACCTGCAGCCGGTGTTCTCCTTCAAGATCCGCGGCGCCTACACCCGTGTCGCGCGCCTGAGCGACGAGCAGAAGGCCCGC
GGGGTGATCACCGCCTCCGCCGGCAACCATGCCCAGGGCCTGGCCCTGGCGGCGCAGCGCCTGGGCGTGCGGGCGGTGAT
CGTGATGCCGCGCACCACTCCCGAACTCAAGGTCAAGGGCGTGCTGGCACGCGGCGGCGAGGCGTTGCTGCACGGCGACG
CCTTCCCCGATGCGCTGGCCCATGCCCTGCAACTGGCCGAGCGCGAAGGCATGACCTTCGTCCCACCCTACGACGATCCG
GACGTGATCGCCGGACAGGGCACGGTGGCGATGGAGATCCTCCGCCAGCACAGCGGCCGCCTCGACGCGATCTTCGTGCC
GGTCGGCGGCGGCAGCCTGATCGCCGGGATCGCCGCCTACGTCAAGCACCTGCGTCCGGATATTCGCGTGATCGGCGTCG
AGCCGGAGGATTCCAACTGCCTGCAGGCGGCGCTGGCCGCCGGCGAACGGGTGGTGCTGGGCCAGGTCGGGCTGTTCGCC
GACGGGGTGGCGGTGGCGCAGATCGGCGCTTGCAACTTCGAGGTCTGCAAGGACCATGTCGACGAGGTGATCACCGTCGG
CAGCGACGAAATCTGCGCGGCGATCAAGGACATCTACGACGACACCCGTTCGATCACCGAGCCGGCCGGCGCCCTGGCCG
TAGCGGGGATCAAGAAGTACGTGGCCCGCGAACGCACCGAGGGGCAGACCCTGGTGGCCATCGACTCCGGTGCCAACATC
AACTTCGACCGCCTGCGCCACGTTGCCGAGCGCGCCGAACTGGGCGAACAGCGCGAAGCGATCATCGCCGTCACCGTGGC
CGAGCGGCCGGGCAGCTTCAAGGCCTTCTGCGCGGCGCTGGGCCGGCGCCAGATCACCGAGTTCAACTACCGCTACCACA
GCGACCGCCAGGCCCACCTGTTCGTCGGCGTGCAGACCCATCCGCTCACCGACAGCCGCGCCGACCTGCTCGCCGGCCTG
CGCGAGCAGGGCTTCCCGGTACTCGACCTGACCGACAACGAGATGGCCAAGCTGCACATCCGCCACATGGTCGGCGGCCA
CGGTACCGAGGTGCGCCGCGAGCGGCTGTTCCGCTTCGAGTTTCCCGAACGCCCCGGGGCCTTGCTGAACTTCCTCGACA
AGCTCGGCTCGCGCTGGAACATCAGCCTGTTCCACTACCGCAACCACGGCGCCGCCGACGGTCGGGTGCTGGCCGGATTG
CAGGTGCCGGACGAGGAGCGCGGCGAGCTGGAGGCGGCGTTGCAAGCCATCGGCTATCCGTACTGGGAGGAAACCCACAA
CCCGGCCTACCGGCTGTTCGCCGGTTGA