Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00467 experimental
T6SS type Type ii
Strain Francisella tularensis subsp. holarctica LVS
Replicon (RefSeq) chromosome (NC_007880)
Location 1096796..1118939
Function anti-eukaryotic; effector translocation; virulence
Comment Growth, phagosomal escape and cytopathogenicity in cultured macrophages; virulence in mouse model. IglE of F. novicida is required for T6SS in vitro.
References
[1] Nano FE et al (2007) The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]
[2] Broms JE et al (2012) Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631]
[3] Lindgren M et al (2013) Importance of PdpC, IglC, IglI, and IglG for modulation of a host cell death pathway induced by Francisella tularensis LVS. Infect Immun. 81(6):2076-84. [PudMed:23529623]
[4] Broms JE et al (2011) IglG and IglI of the Francisella pathogenicity island are important virulence determinants of Francisella tularensis LVS. Infect Immun. 79(9):3683-96. [PudMed:21690239]
[5] Lindgren M et al (2013) The Francisella tularensis LVS DeltapdpC mutant exhibits a unique phenotype during intracellular infection. BMC Microbiol. 13(1):20. [PudMed:23356941]
[6] de Bruin OM et al (2011) The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology. 157(Pt 12):3483-91. [PudMed:21980115]
[7] Broms JE et al (2012) DotU and VgrG, core components of type VI secretion systems, are essential for Francisella LVS pathogenicity. PLoS One. 7(4):e34639. [PudMed:22514651]
[8] Brms JE, Meyer L, Sjstedt A. et al (2017) A mutagenesis-based approach identifies amino acids in the N-terminal part of Francisella tularensis IglE that critically control Type VI system-mediated secretion.. Virulence. 8(6):821-847. [PudMed:27830989]
experimental Experimental investigation has been performed.
T6SS components and genome coordinates

ComponentIglCIglDIglEIglFIglGIglHIglIIglJPdpAPdpBPdpCPdpDPdpETssBTssCTssITssL
Number11111111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
FTL_RS05850 1091858..1092922 + 1065 WP_003016146.1 fructose-bisphosphate aldolase class II
FTL_RS10420 1093090..1094543 - 1454 pseudogene alpha,alpha-trehalase
FTL_RS05860 1094545..1094802 - 258 WP_003016148.1 hypothetical protein
FTL_RS10425 1094950..1095830 + 881 WP_011457326.1 IS630 family transposase
FTL_RS05875 1095831..1096703 - 873 WP_011457343.1 MFS transporter
FTL_RS10895 1096688..1097410 + 723 pseudogene Pathogenicity determinant protein D
T6CP004833 TssB FTL_RS05890 (tssB) 1097491..1098045 + 555 WP_003016161.1 type VI secretion system contractile sheath small subunit
T6CP004834 TssC FTL_RS05895 (tssC) 1098059..1099579 + 1521 WP_010031646.1 type VI secretion system contractile sheath large subunit
T6CP004835 IglC FTL_RS05900 1099596..1100225 + 630 WP_003016165.1 hypothetical protein
T6CP004836 IglD FTL_RS05905 1100246..1101442 + 1197 WP_011457345.1 hypothetical protein
T6CP004837 PdpE FTL_RS05910 1101449..1102024 - 576 WP_003016169.1 hypothetical protein
T6CP004838 PdpC FTL_RS05915 (pdpC) 1102042..1106028 - 3987 WP_003016172.1 pathogenicity determinant protein PdpC
T6CP004839 IglJ FTL_RS05920 1106068..1106841 - 774 WP_003016175.1 hypothetical protein
T6CP004840 IglI FTL_RS05925 1106807..1107958 - 1152 WP_003016179.1 hypothetical protein
T6CP004841 TssL FTL_RS05930 1107980..1108603 - 624 WP_003016181.1 DotU family type IV/VI secretion system protein
T6CP004842 IglH FTL_RS05935 1108616..1110046 - 1431 WP_003016182.1 hypothetical protein
T6CP004843 IglG FTL_RS05940 1110059..1110580 - 522 WP_003016185.1 DUF4280 domain-containing protein
T6CP004844 IglF FTL_RS05945 1110648..1112312 - 1665 WP_003016188.1 hypothetical protein
T6CP004845 TssI FTL_RS05950 1112317..1112811 - 495 WP_003016192.1 hypothetical protein
T6CP004846 IglE FTL_RS05955 1112824..1113201 - 378 WP_003018235.1 hypothetical protein
T6CP004847 PdpB FTL_RS05960 1113191..1116472 - 3282 WP_011457346.1 hypothetical protein
T6CP004848 PdpA FTL_RS05965 1116477..1118939 - 2463 WP_003016195.1 hypothetical protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF00565 experimental FTT_1347 (VgrG) - - Francisella tularensis subsp. holarctica LVS chromosome (NC_007880) 1112317..1112811 (-) 89256491
EFF00564 experimental FTL_0118 (IglI) - - Francisella tularensis subsp. holarctica LVS chromosome (NC_007880) 1106807..1107958 (-) 89256486

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector