Detailed information of effector
Summary
| Effector ID | EFF01517  |
| Effector type | DddA |
| Cognate immunity protein | - |
| Related T6SS (Type) | - |
| Strain | Burkholderia cenocepacia J2315 |
| Replicon | chromosome |
| Sequence | Protein sequence (160 a.a.); Nucleotide sequence (480 bp) |
| Description | TecA deamidation of RhoA mediates B. cenocepacia-induced pyrin inflammasome activation |
| Reference | - |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | BCAM1857 (TecA) |
| Coordinates (Strand) | 2076087..2076566 (+) |
| NCBI ID | 206563705 |
| RefSeq | NC_011001 |
| Uniprot ID | UPI00017BA05A |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-159 | LQARQMDANGRAIVRKAAGRIRLQRPCKVDRIHPSGAQQPAVDRANPGAADRNRRMKDIAAAREADRGARRNARAPVPLIAGFEVEPRPPRLTGRIEQRIGRDQRVCTQQIADIGRGVVADAAGYVFVDVRHPEHQRLRVFLPFGDAGLGDMPA*LGQL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9464 | - | - |
Protein sequence: 160 a.a. .
>EFF01517 NC_011001:2076087-2076566 DS-DNA Deaminase toxin A (DddA) [Burkholderia cenocepacia J2315]
LQARQMDANGRAIVRKAAGRIRLQRPCKVDRIHPSGAQQPAVDRANPGAADRNRRMKDIAAAREADRGARRNARAPVPLI
AGFEVEPRPPRLTGRIEQRIGRDQRVCTQQIADIGRGVVADAAGYVFVDVRHPEHQRLRVFLPFGDAGLGDMPA*LGQLH
LQARQMDANGRAIVRKAAGRIRLQRPCKVDRIHPSGAQQPAVDRANPGAADRNRRMKDIAAAREADRGARRNARAPVPLI
AGFEVEPRPPRLTGRIEQRIGRDQRVCTQQIADIGRGVVADAAGYVFVDVRHPEHQRLRVFLPFGDAGLGDMPA*LGQLH
Nucleotide sequence: 480 bp .
>EFF01517 NC_011001:2076087-2076566 DS-DNA Deaminase toxin A (DddA) [Burkholderia cenocepacia J2315]
CTACAGGCGCGACAGATGGACGCGAATGGTCGTGCGATCGTACGTAAAGCCGCCGGCCGGATCCGGCTGCAGCGCCCGTG
CAAGGTCGACCGGATTCATCCATCCGGCGCCCAGCAGCCCGCCGTTGATCGCGCGAATCCGGGTGCCGCCGACCGCAATC
GCCGCATGAAAGACATTGCCGCCGCGCGCGAAGCCGACCGCGGTGCCCGCCGGAATGCTCGCGCGCCCGTCCCACTGATC
GCCGGTTTCGAAGTTGAACCGCGTCCTCCACGATTGACCGGCCGTATCGAGCAGCGCATCGGGCGCGATCAGCGCGTCTG
CACGCAGCAGATAGCGGACATAGGCCGCGGCGTCGTAGCAGACGCCGCGGGATACGTATTCGTCGATGTTCGCCATCCCG
AACATCAGCGCCTGCGCGTGTTTTTGCCGTTCGGCGATGCCGGATTGGGCGACATGCCCGCCTAACTGGGTCAGCTGCAC
CTACAGGCGCGACAGATGGACGCGAATGGTCGTGCGATCGTACGTAAAGCCGCCGGCCGGATCCGGCTGCAGCGCCCGTG
CAAGGTCGACCGGATTCATCCATCCGGCGCCCAGCAGCCCGCCGTTGATCGCGCGAATCCGGGTGCCGCCGACCGCAATC
GCCGCATGAAAGACATTGCCGCCGCGCGCGAAGCCGACCGCGGTGCCCGCCGGAATGCTCGCGCGCCCGTCCCACTGATC
GCCGGTTTCGAAGTTGAACCGCGTCCTCCACGATTGACCGGCCGTATCGAGCAGCGCATCGGGCGCGATCAGCGCGTCTG
CACGCAGCAGATAGCGGACATAGGCCGCGGCGTCGTAGCAGACGCCGCGGGATACGTATTCGTCGATGTTCGCCATCCCG
AACATCAGCGCCTGCGCGTGTTTTTGCCGTTCGGCGATGCCGGATTGGGCGACATGCCCGCCTAACTGGGTCAGCTGCAC