Detailed information of component protein
Summary
| Component ID | T6CP016001 |
| Component protein type | TssB |
| T6SS ID (Type) | T6SS01289  (Type i1) |
| Strain | Vibrio cholerae DL4211 |
| Replicon | chromosome |
| Sequence | Protein sequence (169 a.a.); Nucleotide sequence (507 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | BLX44_RS02405 |
| Coordinate (Strand) | 77226..77732 (-) |
| NCBI ID | WP_000031391.1 |
| RefSeq | NZ_MOLL01000005 |
| Uniprot ID | Q9KN58_VIBCH, A0A0X1L1S0_VIBCO, D7HAV3_VIBCL, A0A0K9UVI4_VIBCL, A0A7U8ZSI9_VIBCL, A0A0H3QAN1_VIBCL, C3LUD1_VIBCM, A0A085TAW9_VIBCL |
| KEGG ID | vcm:VCM66_A0105, vcq:EN18_04260, vcx:VAA049_1801, vcz:VAB027_922, vch:VCA0107 |
| PDB ID | 3J9G, 5MXN, 5MYU, 5OJQ |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_VipA | PF05591.14 | 1.1e-56 | 6..157 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-168 | MSKEGSVAPKERINIKYIPATGDAQAEVELPLKTLVVGDFKGHAEQTPLEERATVTVDKNNFEAVMRESELKITATVKNKLTDDENAELPVELNFKSLADFAPDAVASQVPELKKLIELREALVALKGPLGNIPAFRERLQSLLNSEESREKLLAELNLLSGQEEPQA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9881 | - | - |
Protein sequence: 169 a.a.
>T6CP016001 NZ_MOLL01000005:c77732-77226 [Vibrio cholerae] [TssB]
MSKEGSVAPKERINIKYIPATGDAQAEVELPLKTLVVGDFKGHAEQTPLEERATVTVDKNNFEAVMRESELKITATVKNK
LTDDENAELPVELNFKSLADFAPDAVASQVPELKKLIELREALVALKGPLGNIPAFRERLQSLLNSEESREKLLAELNLL
SGQEEPQA*
MSKEGSVAPKERINIKYIPATGDAQAEVELPLKTLVVGDFKGHAEQTPLEERATVTVDKNNFEAVMRESELKITATVKNK
LTDDENAELPVELNFKSLADFAPDAVASQVPELKKLIELREALVALKGPLGNIPAFRERLQSLLNSEESREKLLAELNLL
SGQEEPQA*
Nucleotide sequence: 507 bp
>T6CP016001 NZ_MOLL01000005:c77732-77226 [Vibrio cholerae] [TssB]
ATGTCTAAAGAAGGAAGTGTAGCTCCCAAAGAGCGGATTAATATCAAGTATATTCCGGCGACGGGGGATGCACAGGCTGA
GGTTGAGCTACCACTCAAAACCCTAGTTGTAGGTGATTTCAAAGGGCATGCGGAGCAAACCCCATTGGAAGAGCGTGCAA
CAGTCACGGTAGATAAGAACAACTTTGAAGCCGTAATGCGCGAGAGCGAGCTGAAAATCACCGCCACGGTGAAAAACAAG
CTGACTGATGATGAGAATGCCGAGCTTCCTGTTGAACTCAATTTCAAATCCTTAGCCGACTTCGCTCCTGATGCGGTGGC
ATCACAAGTTCCAGAACTGAAAAAATTGATTGAGTTGCGTGAAGCGTTAGTTGCCCTCAAAGGGCCGCTAGGCAACATTC
CCGCATTTCGTGAGCGTTTACAGTCATTACTCAACTCAGAAGAGTCGAGAGAAAAACTGTTGGCAGAACTGAATCTGCTC
AGTGGTCAAGAAGAGCCACAAGCGTAA
ATGTCTAAAGAAGGAAGTGTAGCTCCCAAAGAGCGGATTAATATCAAGTATATTCCGGCGACGGGGGATGCACAGGCTGA
GGTTGAGCTACCACTCAAAACCCTAGTTGTAGGTGATTTCAAAGGGCATGCGGAGCAAACCCCATTGGAAGAGCGTGCAA
CAGTCACGGTAGATAAGAACAACTTTGAAGCCGTAATGCGCGAGAGCGAGCTGAAAATCACCGCCACGGTGAAAAACAAG
CTGACTGATGATGAGAATGCCGAGCTTCCTGTTGAACTCAATTTCAAATCCTTAGCCGACTTCGCTCCTGATGCGGTGGC
ATCACAAGTTCCAGAACTGAAAAAATTGATTGAGTTGCGTGAAGCGTTAGTTGCCCTCAAAGGGCCGCTAGGCAACATTC
CCGCATTTCGTGAGCGTTTACAGTCATTACTCAACTCAGAAGAGTCGAGAGAAAAACTGTTGGCAGAACTGAATCTGCTC
AGTGGTCAAGAAGAGCCACAAGCGTAA