Detailed information of component protein
Summary
| Component ID | T6CP015993 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS01289  (Type i1) |
| Strain | Vibrio cholerae DL4211 |
| Replicon | chromosome |
| Sequence | Protein sequence (258 a.a.); Nucleotide sequence (774 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | BLX44_RS02365 |
| Coordinate (Strand) | 68429..69202 (-) |
| NCBI ID | 446005552 |
| RefSeq | NZ_MOLL01000005 |
| Uniprot ID | D7HAU5_VIBCL, A0A023PTG4_VIBCL |
| KEGG ID | vcx:VAA049_1793, vcz:VAB027_914 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 2.5e-61 | 39..240 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-219 | MSQSKKETPLASLLFDDVEKINHDQDYWFQLRGDNPNVLIDAATPLFGLSLRVRTLTECDNIEQIYRQTIEEIKAIEIELTEQGYEHAILMAYRYILCAFLDESVMGTEWGASSLWAEHSMLSRFHNETWGGEKVFTILSRLEGEPHRYQALLAFIYHCLILGFEGKYRVMEGGQAEREKVISRLHQLLSSLEESEPQDLTRPTDHVVRAKYTLSRQMP |
| Transmembrane helix | 220-242 | VWSVFAGFIVLWIGLFLGYSYVL |
| Outside | 243-257 | HSKSSDVLNQLNQIL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9933 | - | - |
Protein sequence: 258 a.a.
>T6CP015993 NZ_MOLL01000005:c69202-68429 [Vibrio cholerae] [TssL]
MSQSKKETPLASLLFDDVEKINHDQDYWFQLRGDNPNVLIDAATPLFGLSLRVRTLTECDNIEQIYRQTIEEIKAIEIEL
TEQGYEHAILMAYRYILCAFLDESVMGTEWGASSLWAEHSMLSRFHNETWGGEKVFTILSRLEGEPHRYQALLAFIYHCL
ILGFEGKYRVMEGGQAEREKVISRLHQLLSSLEESEPQDLTRPTDHVVRAKYTLSRQMPVWSVFAGFIVLWIGLFLGYSY
VLHSKSSDVLNQLNQIL*
MSQSKKETPLASLLFDDVEKINHDQDYWFQLRGDNPNVLIDAATPLFGLSLRVRTLTECDNIEQIYRQTIEEIKAIEIEL
TEQGYEHAILMAYRYILCAFLDESVMGTEWGASSLWAEHSMLSRFHNETWGGEKVFTILSRLEGEPHRYQALLAFIYHCL
ILGFEGKYRVMEGGQAEREKVISRLHQLLSSLEESEPQDLTRPTDHVVRAKYTLSRQMPVWSVFAGFIVLWIGLFLGYSY
VLHSKSSDVLNQLNQIL*
Nucleotide sequence: 774 bp
>T6CP015993 NZ_MOLL01000005:c69202-68429 [Vibrio cholerae] [TssL]
ATGTCACAGAGTAAAAAAGAGACGCCATTAGCCAGTTTGTTGTTCGACGATGTGGAAAAAATTAACCACGACCAAGACTA
CTGGTTCCAATTGCGTGGGGACAATCCGAATGTGCTGATTGATGCCGCGACACCCTTGTTTGGGCTTTCTCTGCGGGTTC
GCACCCTCACTGAATGCGACAACATTGAACAGATCTACCGTCAGACGATTGAAGAGATCAAAGCGATTGAAATTGAGTTG
ACGGAGCAAGGTTACGAACACGCCATTTTGATGGCCTATCGCTACATCTTGTGCGCCTTTTTGGATGAATCCGTGATGGG
AACCGAGTGGGGCGCGTCCAGTTTATGGGCCGAGCACTCCATGCTGTCACGTTTCCATAATGAAACGTGGGGCGGTGAGA
AAGTGTTTACGATTTTGAGCCGTCTTGAGGGTGAACCACACCGTTACCAAGCGCTATTGGCCTTTATTTATCACTGCCTG
ATTTTGGGCTTTGAAGGCAAATACCGTGTGATGGAAGGGGGACAAGCTGAGCGCGAAAAAGTGATCAGCCGCCTGCATCA
ACTGCTGAGTAGCTTGGAAGAGAGCGAGCCACAAGACTTAACCCGTCCGACCGATCATGTGGTGCGTGCGAAATATACCC
TAAGCAGACAGATGCCTGTTTGGTCTGTGTTTGCTGGCTTTATCGTGCTTTGGATAGGTCTATTTCTCGGCTACAGCTAT
GTGCTGCATAGCAAATCCAGCGATGTGCTCAATCAACTCAATCAAATACTTTAA
ATGTCACAGAGTAAAAAAGAGACGCCATTAGCCAGTTTGTTGTTCGACGATGTGGAAAAAATTAACCACGACCAAGACTA
CTGGTTCCAATTGCGTGGGGACAATCCGAATGTGCTGATTGATGCCGCGACACCCTTGTTTGGGCTTTCTCTGCGGGTTC
GCACCCTCACTGAATGCGACAACATTGAACAGATCTACCGTCAGACGATTGAAGAGATCAAAGCGATTGAAATTGAGTTG
ACGGAGCAAGGTTACGAACACGCCATTTTGATGGCCTATCGCTACATCTTGTGCGCCTTTTTGGATGAATCCGTGATGGG
AACCGAGTGGGGCGCGTCCAGTTTATGGGCCGAGCACTCCATGCTGTCACGTTTCCATAATGAAACGTGGGGCGGTGAGA
AAGTGTTTACGATTTTGAGCCGTCTTGAGGGTGAACCACACCGTTACCAAGCGCTATTGGCCTTTATTTATCACTGCCTG
ATTTTGGGCTTTGAAGGCAAATACCGTGTGATGGAAGGGGGACAAGCTGAGCGCGAAAAAGTGATCAGCCGCCTGCATCA
ACTGCTGAGTAGCTTGGAAGAGAGCGAGCCACAAGACTTAACCCGTCCGACCGATCATGTGGTGCGTGCGAAATATACCC
TAAGCAGACAGATGCCTGTTTGGTCTGTGTTTGCTGGCTTTATCGTGCTTTGGATAGGTCTATTTCTCGGCTACAGCTAT
GTGCTGCATAGCAAATCCAGCGATGTGCTCAATCAACTCAATCAAATACTTTAA