SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015988
Component protein type	TssI
T6SS ID (Type)	T6SS01289 (Type i1)
Strain	Vibrio cholerae DL4211
Replicon	chromosome
Sequence	Protein sequence (977 a.a.); Nucleotide sequence (2931 bp)
Reference	[1] Unterweger D, Kitaoka M, Miyata ST, et al. Constitutive type VI secretion system expression gives Vibrio cholerae intra- and interspecific competitive advantages. PLoS One. 2012;7(10):e48320. doi: 10.1371/journal.pone.0048320. Epub 2012 Oct 26. PMID: 23110230

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BLX44_RS02325
Coordinate (Strand)	54064..56994 (-)
NCBI ID	1169245860
RefSeq	NZ_MOLL01000005
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	2.1e-70	41..334
Phage_base_V	PF04717.14	4.8e-15	391..458
Phage_lysozyme	PF00959.21	2.3e-12	851..962
PG_binding_1	PF01471.20	4.2e-08	731..773

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-976	MARLQFQLKVDGLEDESLVVRGFEGQESLSDSVWRSEPCYGFRYQVDLASALSNLTAEQFVDQTAHLTILRDGQVVQQINGIVRQFSKGDTGHRHTFYSLTLVPALERLSLRSNSRIFQQQSVPEIISILLQEMGIEDYAFALKRECAQREFCVQYRETDLQFLHRIAAEEGLVYSHLHEAQKHTLLFTDSSDSQPKLAKPVPYNALAGGEINLPYVVDLQFKTTAQVSHTELKDYSFKKPAYGFTQRTQGKDIAYQQPNYEHFDAPGRYKDDTNGKAFSQIRLEYLRRDALLADAKSDEPLLLAGVRFDLQDHLDHAMNRDWLVVQANHQGTQPQALQEEGGAGATTYSNQLKLIPAHITWRARPCAKPQVDGPMIATVVGPQGEEIYCDNFGRVKVHFPWDRYSSSNEKSSCWVRVAQEWAGSQYGSMAIPRVGHEVIVSFLNGDPDQPIITGRTYHATNTAPYVLPDHKTKTVLRTETHQGQGYNELSFEDQAGSEQILLHAQKDWDALIEHDHTEVIRHDQHLTVDNDRFIRIQRNQHVTIEGESRSKIVLDSSHEVGASLQHKVGQRIAVEAGKEISLKSGAKIVVEAGAELTLKAGGSFVKVDAGGVHLVGPAINLNAGGSAGSGSAYGGQLAAAPRMLAQAKPVAELVQPDIAASMQSGAARVIDVASLPTMMPSSANNTANGEPVAEEKTPERILKSDLLKPSDELEKLAKRQAGAYRQGNHSDEVKLLQEALIKLGFDLGKAGADGDFGSKTKTAIEQFQKSYQPSHQTHPSYSIGPVDGIVGKGTLLALDEALMDGWVDSKRSLVFSSNGMALLREIEELRLIPYDDQTGKSISSYVEGATIGYGYLIPKNEWSIYKNGITKEIAEELFLRKVPRFQENVQSKIKVDINQNQFDALVLLCYNIGEGNFNTSSVLKLVNGGSAPVYGNNIDNAWLAWNKSQGKVMQGLIKRRKCELNVYHKGVYEKW

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9958	-	-

Protein sequence: 977 a.a.

						>T6CP015988 NZ_MOLL01000005:c56994-54064 [Vibrio cholerae] [TssI]
MARLQFQLKVDGLEDESLVVRGFEGQESLSDSVWRSEPCYGFRYQVDLASALSNLTAEQFVDQTAHLTILRDGQVVQQIN
GIVRQFSKGDTGHRHTFYSLTLVPALERLSLRSNSRIFQQQSVPEIISILLQEMGIEDYAFALKRECAQREFCVQYRETD
LQFLHRIAAEEGLVYSHLHEAQKHTLLFTDSSDSQPKLAKPVPYNALAGGEINLPYVVDLQFKTTAQVSHTELKDYSFKK
PAYGFTQRTQGKDIAYQQPNYEHFDAPGRYKDDTNGKAFSQIRLEYLRRDALLADAKSDEPLLLAGVRFDLQDHLDHAMN
RDWLVVQANHQGTQPQALQEEGGAGATTYSNQLKLIPAHITWRARPCAKPQVDGPMIATVVGPQGEEIYCDNFGRVKVHF
PWDRYSSSNEKSSCWVRVAQEWAGSQYGSMAIPRVGHEVIVSFLNGDPDQPIITGRTYHATNTAPYVLPDHKTKTVLRTE
THQGQGYNELSFEDQAGSEQILLHAQKDWDALIEHDHTEVIRHDQHLTVDNDRFIRIQRNQHVTIEGESRSKIVLDSSHE
VGASLQHKVGQRIAVEAGKEISLKSGAKIVVEAGAELTLKAGGSFVKVDAGGVHLVGPAINLNAGGSAGSGSAYGGQLAA
APRMLAQAKPVAELVQPDIAASMQSGAARVIDVASLPTMMPSSANNTANGEPVAEEKTPERILKSDLLKPSDELEKLAKR
QAGAYRQGNHSDEVKLLQEALIKLGFDLGKAGADGDFGSKTKTAIEQFQKSYQPSHQTHPSYSIGPVDGIVGKGTLLALD
EALMDGWVDSKRSLVFSSNGMALLREIEELRLIPYDDQTGKSISSYVEGATIGYGYLIPKNEWSIYKNGITKEIAEELFL
RKVPRFQENVQSKIKVDINQNQFDALVLLCYNIGEGNFNTSSVLKLVNGGSAPVYGNNIDNAWLAWNKSQGKVMQGLIKR
RKCELNVYHKGVYEKW*					

Nucleotide sequence: 2931 bp

						>T6CP015988 NZ_MOLL01000005:c56994-54064 [Vibrio cholerae] [TssI]
ATGGCAAGGTTACAGTTTCAATTAAAGGTGGATGGCCTTGAGGATGAATCCCTCGTCGTACGCGGATTTGAAGGGCAAGA
GTCCTTGTCTGATTCTGTATGGCGCAGTGAACCCTGCTATGGTTTTCGCTATCAGGTTGATTTAGCCAGTGCGCTAAGCA
ACCTTACTGCTGAGCAATTTGTTGACCAAACCGCGCATTTAACCATTCTGCGTGACGGGCAAGTGGTGCAGCAAATAAAC
GGTATTGTGCGTCAATTCAGCAAAGGAGATACCGGCCATCGACATACTTTCTATTCATTAACGCTAGTTCCTGCGTTGGA
AAGGCTTTCTCTGCGTAGTAATAGCCGTATTTTCCAGCAGCAAAGCGTGCCAGAGATTATCTCTATTTTACTGCAAGAGA
TGGGGATTGAAGATTACGCCTTTGCCCTTAAGCGTGAGTGTGCCCAGCGAGAGTTTTGTGTACAGTATCGTGAAACGGAT
TTGCAGTTTTTACACCGTATCGCCGCCGAAGAAGGCTTGGTTTACAGCCACCTACATGAAGCGCAGAAACACACCTTGTT
GTTTACCGATAGCTCGGACAGCCAACCGAAGTTAGCCAAGCCAGTACCTTATAATGCGCTGGCGGGTGGCGAGATCAATC
TCCCTTATGTGGTCGATCTACAATTCAAGACCACCGCGCAAGTCAGCCATACCGAACTTAAAGATTACAGCTTTAAGAAG
CCAGCCTACGGATTTACTCAGCGCACGCAGGGTAAAGACATCGCTTATCAGCAGCCAAATTATGAGCATTTTGATGCTCC
GGGACGCTACAAAGACGATACAAATGGCAAAGCCTTTAGCCAAATTCGCTTAGAGTATTTACGCCGCGATGCTTTGCTTG
CTGATGCGAAGAGTGACGAACCTTTGCTGCTGGCGGGAGTGCGTTTTGACTTGCAAGATCACCTTGATCACGCAATGAAT
CGAGATTGGCTCGTGGTACAAGCCAATCACCAAGGGACGCAGCCACAAGCGTTACAAGAAGAGGGGGGGGCAGGCGCTAC
CACTTACAGCAATCAGCTAAAACTTATCCCTGCTCACATCACTTGGCGAGCAAGGCCTTGTGCTAAGCCGCAAGTGGATG
GTCCCATGATCGCCACCGTGGTTGGGCCACAAGGTGAAGAGATTTATTGCGATAACTTTGGTCGTGTGAAAGTGCATTTC
CCGTGGGATCGCTACTCAAGTAGCAATGAGAAAAGCTCTTGCTGGGTGCGAGTGGCACAAGAATGGGCGGGTAGCCAATA
CGGTAGTATGGCGATTCCGCGAGTCGGCCATGAAGTGATTGTTTCGTTCCTGAATGGCGATCCGGATCAACCCATCATCA
CGGGGCGTACGTACCATGCGACCAATACCGCGCCTTACGTCTTGCCTGATCACAAAACCAAAACCGTGCTGCGCACTGAG
ACTCACCAAGGGCAAGGCTACAACGAACTGAGTTTTGAGGATCAAGCGGGCAGCGAACAGATTTTGCTGCATGCACAAAA
AGATTGGGATGCGTTGATTGAGCATGATCACACCGAAGTGATTCGCCACGATCAGCATCTCACTGTGGATAATGACCGCT
TTATTCGCATCCAGCGCAATCAACACGTTACCATCGAAGGCGAAAGCCGTAGCAAAATCGTACTCGATAGCAGCCATGAA
GTCGGCGCGTCACTACAACACAAAGTCGGGCAACGCATTGCTGTCGAGGCGGGTAAAGAGATTTCACTCAAAAGCGGCGC
AAAAATCGTGGTTGAAGCCGGAGCGGAGTTAACCCTAAAAGCAGGGGGCAGTTTTGTAAAAGTCGATGCAGGTGGCGTAC
ATCTAGTCGGTCCTGCCATCAACCTAAACGCGGGCGGCAGTGCAGGCAGCGGTAGCGCTTATGGTGGGCAATTAGCGGCA
GCACCAAGAATGTTAGCGCAAGCTAAACCAGTAGCAGAATTGGTTCAGCCGGATATTGCTGCCTCAATGCAATCAGGCGC
GGCGCGTGTTATTGATGTGGCATCACTCCCCACTATGATGCCGAGTTCAGCCAATAACACGGCAAATGGTGAGCCTGTGG
CTGAAGAGAAAACACCGGAGCGAATTTTAAAATCCGATCTCCTCAAACCATCCGATGAGTTAGAGAAACTTGCCAAGCGA
CAAGCGGGTGCTTATCGTCAAGGTAATCACAGCGATGAGGTTAAGTTATTACAAGAAGCGCTCATAAAACTGGGGTTTGA
TCTCGGGAAAGCAGGAGCTGATGGTGACTTTGGGAGCAAAACTAAAACCGCGATTGAGCAGTTCCAGAAAAGCTATCAAC
CAAGCCACCAAACCCACCCGTCTTACAGCATTGGCCCCGTTGACGGTATTGTCGGCAAAGGTACACTACTGGCTCTAGAC
GAAGCTTTGATGGATGGGTGGGTTGATAGCAAAAGGTCTCTAGTATTCTCTTCAAATGGAATGGCTTTACTCAGAGAAAT
AGAAGAACTTCGCCTAATCCCATATGATGACCAAACGGGAAAAAGCATTAGTTCTTACGTAGAAGGTGCAACGATTGGAT
ACGGTTACTTAATACCTAAAAATGAATGGAGTATATACAAAAATGGAATAACGAAAGAGATTGCTGAGGAACTATTTTTA
AGGAAAGTCCCTAGATTTCAAGAAAATGTTCAATCAAAAATTAAAGTTGATATCAACCAAAATCAATTCGATGCATTGGT
TTTGCTTTGTTATAACATTGGAGAAGGTAACTTTAATACATCATCAGTTTTAAAATTGGTGAATGGAGGAAGTGCTCCAG
TATATGGAAACAATATAGATAATGCATGGTTAGCGTGGAATAAATCACAAGGTAAAGTAATGCAAGGACTTATTAAAAGA
AGAAAGTGTGAACTAAATGTCTATCACAAAGGAGTATATGAAAAATGGTAA					