SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015887
Component protein type	TssG
T6SS ID (Type)	T6SS01278 (Type i1)
Strain	Aliivibrio fischeri strain FQ-A001
Replicon	chromosome
Sequence	Protein sequence (333 a.a.); Nucleotide sequence (999 bp)
Reference	[1] Guckes KR, Cecere AG, Wasilko NP, et al. Incompatibility of Vibrio fischeri Strains during Symbiosis Establishment Depends on Two Functionally Redundant hcp Genes. J Bacteriol. 2019 Sep 6;201(19). pii: e00221-19. doi: 10.1128/JB.00221-19. Print 2019 Oct 1. PMID: 31331977 [2] Speare L, Cecere AG, Guckes KR, et al. Bacterial symbionts use a type VI secretion system to eliminate competitors in their natural host. Proc Natl Acad Sci U S A. 2018 Sep 4;115(36):E8528-E8537. doi: 10.1073/pnas.1808302115. Epub 2018 Aug 20. PMID: 30127013

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VFFQA001_RS15545
Coordinate (Strand)	339523..340521 (-)
NCBI ID	1027713318
RefSeq	NZ_SJSX01000004
Uniprot ID	UPI0007C5310C
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	2.1e-85	9..317

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-332	MIQYIVNKPCDYSFFQAVSLLEKHYQNLPGIEYKAIGENQYVKNEYIQFSVSPKLSFPKSDIEFISEFERNDAVYTQIEVNFMGLHGVSSPLPASYTEKLAGRDPDDNPVKEFFDFFHHRYISMLYRVWKKYRYHIQYKSDAKDPFSGRLLHLVGLSSVIQESSEVDLDRAKLLSYVNQLSTRTRSPKLVSGIVSHYFSLSNVYIEEWVYRRITIAASQRNCLNRKNCQLGGDFHLGQSMADLTGKFNLCIDDIDFDTYVKFLPGGALHETLIGLMKFILCDPMAWDLKLTVRNKSLPINQLGDGLGNELGQTFWLGTTKGEETKIKIVGSN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9955	-	-

Protein sequence: 333 a.a.

						>T6CP015887 NZ_SJSX01000004:c340521-339523 [Aliivibrio fischeri] [TssG]
MIQYIVNKPCDYSFFQAVSLLEKHYQNLPGIEYKAIGENQYVKNEYIQFSVSPKLSFPKSDIEFISEFERNDAVYTQIEV
NFMGLHGVSSPLPASYTEKLAGRDPDDNPVKEFFDFFHHRYISMLYRVWKKYRYHIQYKSDAKDPFSGRLLHLVGLSSVI
QESSEVDLDRAKLLSYVNQLSTRTRSPKLVSGIVSHYFSLSNVYIEEWVYRRITIAASQRNCLNRKNCQLGGDFHLGQSM
ADLTGKFNLCIDDIDFDTYVKFLPGGALHETLIGLMKFILCDPMAWDLKLTVRNKSLPINQLGDGLGNELGQTFWLGTTK
GEETKIKIVGSN*					

Nucleotide sequence: 999 bp

						>T6CP015887 NZ_SJSX01000004:c340521-339523 [Aliivibrio fischeri] [TssG]
ATGATTCAATATATTGTTAACAAACCATGCGATTATAGTTTCTTTCAAGCGGTGTCTTTACTTGAAAAACACTATCAAAA
TTTACCTGGTATTGAATATAAAGCTATTGGTGAGAACCAATACGTAAAGAATGAGTACATTCAATTTAGCGTATCTCCAA
AACTTAGCTTCCCTAAAAGTGATATAGAATTTATTTCTGAATTTGAACGTAACGATGCTGTCTATACACAAATAGAAGTT
AACTTTATGGGACTTCACGGTGTAAGTTCACCATTACCAGCTTCGTATACGGAAAAATTAGCAGGAAGAGATCCTGATGA
TAACCCTGTAAAAGAGTTTTTTGATTTTTTCCATCACAGATACATCAGTATGCTTTATCGAGTATGGAAAAAATATCGGT
ATCATATTCAATATAAGAGTGATGCTAAGGATCCGTTCTCAGGTAGGTTATTACATTTAGTTGGATTATCTAGCGTAATT
CAAGAAAGTAGTGAGGTCGATTTAGACAGAGCGAAATTACTTTCCTATGTTAATCAACTTTCGACGCGTACACGTTCACC
AAAACTTGTTTCTGGTATCGTTTCCCATTACTTTTCGCTTTCAAATGTATACATAGAAGAATGGGTATACAGACGAATTA
CTATTGCGGCTTCTCAACGTAATTGTCTTAATCGAAAGAATTGTCAATTGGGTGGTGACTTCCATTTAGGACAGAGTATG
GCGGATTTAACCGGAAAGTTTAATCTGTGTATTGATGATATTGATTTCGATACCTATGTGAAATTTTTGCCTGGTGGTGC
GTTACATGAAACGTTAATCGGACTAATGAAGTTTATTTTATGTGATCCGATGGCGTGGGATTTAAAACTAACTGTACGAA
ATAAGTCTTTACCTATAAATCAATTAGGTGATGGCTTAGGAAATGAGCTTGGACAAACATTTTGGTTAGGAACGACAAAA
GGGGAGGAGACGAAAATTAAAATAGTAGGCAGTAACTAA					