SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015885
Component protein type	TssL
T6SS ID (Type)	T6SS01278 (Type i1)
Strain	Aliivibrio fischeri strain FQ-A001
Replicon	chromosome
Sequence	Protein sequence (264 a.a.); Nucleotide sequence (792 bp)
Reference	[1] Guckes KR, Cecere AG, Wasilko NP, et al. Incompatibility of Vibrio fischeri Strains during Symbiosis Establishment Depends on Two Functionally Redundant hcp Genes. J Bacteriol. 2019 Sep 6;201(19). pii: e00221-19. doi: 10.1128/JB.00221-19. Print 2019 Oct 1. PMID: 31331977 [2] Speare L, Cecere AG, Guckes KR, et al. Bacterial symbionts use a type VI secretion system to eliminate competitors in their natural host. Proc Natl Acad Sci U S A. 2018 Sep 4;115(36):E8528-E8537. doi: 10.1073/pnas.1808302115. Epub 2018 Aug 20. PMID: 30127013

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VFFQA001_RS15485
Coordinate (Strand)	318668..319459 (+)
NCBI ID	1597593399
RefSeq	NZ_SJSX01000004
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	2.3e-49	47..247

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-230	MSYVEDDVTVVLFQPEPGKPMEVMPAPDLSRNISVSELDISSMGINPLVDQFSWLIASLSCMSSIPWLDDPMPFREQVAREIRKGERMLNEMEVDRASILVIRYCLCAAMDESVCRQEWGANSNWSQNSLLSEFHNETSGGDKFFVILDRLKADPRKYRHVIEFLYLLLQLGFKGKYGRAERGAESLTDIGNTIYRLVRDERLAEQEKVALVDVKSKYLKKPLKRVLPPK
Transmembrane helix	231-253	LILGVSALLFAAMYAATYLVIDV
Outside	254-263	RFQKLLSLYQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9938	-	-

Protein sequence: 264 a.a.

						>T6CP015885 NZ_SJSX01000004:318668-319459 [Aliivibrio fischeri] [TssL]
MSYVEDDVTVVLFQPEPGKPMEVMPAPDLSRNISVSELDISSMGINPLVDQFSWLIASLSCMSSIPWLDDPMPFREQVAR
EIRKGERMLNEMEVDRASILVIRYCLCAAMDESVCRQEWGANSNWSQNSLLSEFHNETSGGDKFFVILDRLKADPRKYRH
VIEFLYLLLQLGFKGKYGRAERGAESLTDIGNTIYRLVRDERLAEQEKVALVDVKSKYLKKPLKRVLPPKLILGVSALLF
AAMYAATYLVIDVRFQKLLSLYQ*					

Nucleotide sequence: 792 bp

						>T6CP015885 NZ_SJSX01000004:318668-319459 [Aliivibrio fischeri] [TssL]
ATGAGTTATGTTGAAGACGACGTAACAGTTGTACTTTTTCAACCTGAACCGGGCAAACCCATGGAGGTTATGCCCGCCCC
TGATTTATCTCGCAATATTTCAGTCAGTGAATTGGATATTTCATCTATGGGGATCAATCCATTAGTTGACCAATTCTCAT
GGCTTATTGCGAGTTTATCTTGTATGTCTTCCATTCCATGGCTTGATGATCCTATGCCTTTTCGTGAGCAAGTAGCTCGT
GAAATACGTAAAGGTGAACGTATGCTCAACGAAATGGAAGTCGACCGTGCATCTATTCTGGTTATCCGTTATTGCTTATG
TGCTGCAATGGATGAATCGGTATGCCGCCAAGAATGGGGAGCCAACAGTAACTGGAGCCAGAACAGCTTATTGTCTGAAT
TTCATAATGAAACCTCAGGTGGCGATAAGTTTTTTGTCATTTTAGATAGATTAAAAGCAGACCCTCGCAAGTATCGCCAT
GTGATTGAATTCCTATATTTACTCCTACAACTTGGTTTTAAAGGTAAATATGGTCGAGCTGAGCGTGGTGCCGAAAGTTT
GACAGATATCGGCAACACTATCTACCGTTTGGTTCGTGACGAGCGTCTTGCTGAACAAGAGAAAGTAGCATTAGTTGATG
TCAAATCTAAGTACTTAAAAAAACCATTAAAACGCGTACTGCCTCCAAAGTTGATCCTTGGCGTCAGTGCTTTACTATTT
GCGGCCATGTATGCCGCTACGTATCTCGTTATTGATGTTCGATTTCAAAAGTTACTGTCTCTTTATCAGTAG					