SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015883
Component protein type	TssJ
T6SS ID (Type)	T6SS01278 (Type i1)
Strain	Aliivibrio fischeri strain FQ-A001
Replicon	chromosome
Sequence	Protein sequence (167 a.a.); Nucleotide sequence (501 bp)
Reference	[1] Guckes KR, Cecere AG, Wasilko NP, et al. Incompatibility of Vibrio fischeri Strains during Symbiosis Establishment Depends on Two Functionally Redundant hcp Genes. J Bacteriol. 2019 Sep 6;201(19). pii: e00221-19. doi: 10.1128/JB.00221-19. Print 2019 Oct 1. PMID: 31331977 [2] Speare L, Cecere AG, Guckes KR, et al. Bacterial symbionts use a type VI secretion system to eliminate competitors in their natural host. Proc Natl Acad Sci U S A. 2018 Sep 4;115(36):E8528-E8537. doi: 10.1073/pnas.1808302115. Epub 2018 Aug 20. PMID: 30127013

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VFFQA001_RS15475
Coordinate (Strand)	316834..317334 (+)
NCBI ID	501528638
RefSeq	NZ_SJSX01000004
Uniprot ID	B5EUI7_ALIFM
KEGG ID	vfm:VFMJ11_A0806
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	4e-29	34..150

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-166	MRKLLTFSLIFLLSGCTMWDQFKESTGITPETSSIELIITADKVLNVREGGQSSPVILRIHELNSPVLFRNLDFFALFENDKDALGDEYIKRYEYQIQPGDVVHETLVLDPATRAIGFSVAFRDINGSSWRKVELIEEKSEYFLKLNLKESELSSNNTRGIEQIYF

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9984	pos: 15-16. LSG-CT.	MRKLLTFSLIFLLSG

Protein sequence: 167 a.a.

						>T6CP015883 NZ_SJSX01000004:316834-317334 [Aliivibrio fischeri] [TssJ]
MRKLLTFSLIFLLSGCTMWDQFKESTGITPETSSIELIITADKVLNVREGGQSSPVILRIHELNSPVLFRNLDFFALFEN
DKDALGDEYIKRYEYQIQPGDVVHETLVLDPATRAIGFSVAFRDINGSSWRKVELIEEKSEYFLKLNLKESELSSNNTRG
IEQIYF*					

Nucleotide sequence: 501 bp

						>T6CP015883 NZ_SJSX01000004:316834-317334 [Aliivibrio fischeri] [TssJ]
ATGCGAAAATTACTCACGTTTTCTCTTATTTTTTTATTATCTGGCTGCACTATGTGGGATCAGTTTAAAGAATCAACAGG
AATTACCCCTGAAACCTCTTCTATCGAGTTAATTATTACAGCAGATAAAGTATTAAATGTTCGTGAAGGTGGACAATCAT
CCCCCGTAATATTGAGGATTCATGAACTAAACTCCCCTGTTCTTTTTCGTAACCTTGATTTCTTTGCGTTATTTGAAAAT
GACAAAGATGCACTTGGTGATGAGTATATAAAACGATACGAATACCAAATTCAGCCTGGGGATGTAGTACATGAGACTCT
GGTATTAGACCCAGCGACTCGAGCTATCGGTTTCTCTGTAGCATTTAGAGACATAAACGGATCATCTTGGCGTAAAGTCG
AGTTAATCGAAGAAAAAAGCGAATACTTCTTAAAGCTAAACCTGAAAGAAAGTGAACTTAGCTCCAACAATACTCGCGGT
ATTGAGCAAATATATTTTTAA					