Detailed information of component protein


Summary

Component ID T6CP015333
Component protein type TssM
T6SS ID (Type) T6SS01211 experimental (Type i1)
Strain Pseudomonas aeruginosa PAK
Replicon chromosome
Sequence Protein sequence (1176 a.a.); Nucleotide sequence (3528 bp)
Reference
[1] Chen R, Weng Y, Zhu F, et al. Polynucleotide Phosphorylase Regulates Multiple Virulence Factors and the Stabilities of Small RNAs RsmY/Z in Pseudomonas aeruginosa. Front Microbiol. 2016 Mar 2;7:247. doi: 10.3389/fmicb.2016.00247. eCollection 2016. PMID: 26973625
[2] Li K, Xu C, Jin Y, et al. SuhB is a regulator of multiple virulence genes and essential for pathogenesis of Pseudomonas aeruginosa. mBio. 2013 Oct 29;4(6):e00419-13. doi: 10.1128/mBio.00419-13. PMID: 24169572
[3] Brencic A, Lory S. Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA. Mol Microbiol. 2009 May;72(3):612-32. doi: 10.1111/j.1365-2958.2009.06670.x. PMID: 19426209
[4] Marden JN, Diaz MR, Walton WG, et al. An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):15055-60. doi: 10.1073/pnas.1307217110. Epub 2013 Aug 26. PMID: 23980177
[5] Aragon IM, Pérez-Mendoza D, Moscoso JA, et al. Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections. Environ Microbiol. 2015 Nov;17(11):4332-51. doi: 10.1111/1462-2920.12856. Epub 2015 Apr 28. PMID: 25809128
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) Y880_RS05405
Coordinate (Strand) 1134277..1137804 (+)
NCBI ID WP_016253577.1
RefSeq NZ_CP020659
Uniprot ID UPI000340892A
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 1.2e-78 198..452
IcmF-related PF06761.14 1.3e-71 507..792
IcmF_C PF06744.14 2e-22 1050..1154
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-16 MKNFFKKVGAFLRKTW
Transmembrane helix 17-39 VWTLLLVLLAALLVWFVGPLLAV
Outside 40-48 ADYKFWESP
Transmembrane helix 49-71 TSRLLSISLMFLVWGLAMVFVSW
Inside 72-447 RAGVRRKAVEDSDEGQERLRRDSLIEDEQKELRSRFKDALRTLKTSSLYRGRSERWRNELPWYLLIGPQGSGKTSLLDFSGLEFPINRIDRKLTRDTAGTRYCDWYFADHGVLIDTAGRYLTQADGEVDGGAWATLLGLLRKRRRARPLNGVMVSIPVETLLSDDEMALDTLARQVRARLQEIQQTLHLDLPVYLVLSKADQLLGFDEFFDQLSREESDQVLGTSFGKEQNGTDVTVLRQAFEDLLRRLNSQVIMRIHQERDTLRRGRILDFPHQLGQIGERLCLFVELAFTGNRYQRASRLRGFYLTSAPHLAQQLDEDTAGIGANLGIASGLLPTLRNGRSRFIHHLLSQVIFPEANLAGLDRRERTRIHWGQR
Transmembrane helix 448-470 ALYVGSLALLLLFGLLWAGGFSA
Outside 471-1175 NHERLERVRELAQQWAQQRQALGERDDAMAALKTLDSSYAATTVFPPKGEVSLHERAGLYQGNEVNPPLAAAYRRELETQLLPRVARMLEAQIRGNMQNRERLLNSLRAYLMLNLRERRENGWLKDWVATDWSQRYAGNGTVQNGLNTHFARLLEQPFSYPLNETLVAQARQVLRSESLANVVYRVLREQARSLPEYRFGQHLGPQAGLFVGTDYVIPGFYTQQGYQQYFVVQGASLVNDILRDNWVLGEGSGISDMDLRRLMVELEQLYFRDYANFWSEAVGRLGLLPFNDAGEGADQVSGLLAANSPILQLLLEVRENTRFPVLAESAEALAEASDKAAEKGGKLGKAAAAAAGKARDALAKNLPDTAKKSLQRRFEPLHRLLDDNNGPAADLTPLLQALNELQLQLAGLARASQPEQAAFDMAKSRMGGQRDALSNLRNASARLPRPLGGWFSVLAEDAWRLVLGDAYLYLNQRYQSELYGFYSKAINKRYPFNAHSASDVAVSDFREFFKAQGVAERFFETYMRPFVSGDPGSYRLRSVDGYSLPMSKVYLDQMGTAQVIRQSFFAENPAEPQVRFRLEPYTLDPGVSRAEFRFDGQSMEYRHGPIVPMSFKWPSDADGGRTSLVLEKMAGRPVGIERNTGPWSLFRLFDLMQTEYLSGRDVMVLKADLGGLRANYLLLAQRTPNPFDLAVLRSFRMPVQL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9955 - -


  Protein sequence: 1176 a.a.    

>T6CP015333 NZ_CP020659:1134277-1137804 [Pseudomonas aeruginosa PAK] [TssM]
MKNFFKKVGAFLRKTWVWTLLLVLLAALLVWFVGPLLAVADYKFWESPTSRLLSISLMFLVWGLAMVFVSWRAGVRRKAV
EDSDEGQERLRRDSLIEDEQKELRSRFKDALRTLKTSSLYRGRSERWRNELPWYLLIGPQGSGKTSLLDFSGLEFPINRI
DRKLTRDTAGTRYCDWYFADHGVLIDTAGRYLTQADGEVDGGAWATLLGLLRKRRRARPLNGVMVSIPVETLLSDDEMAL
DTLARQVRARLQEIQQTLHLDLPVYLVLSKADQLLGFDEFFDQLSREESDQVLGTSFGKEQNGTDVTVLRQAFEDLLRRL
NSQVIMRIHQERDTLRRGRILDFPHQLGQIGERLCLFVELAFTGNRYQRASRLRGFYLTSAPHLAQQLDEDTAGIGANLG
IASGLLPTLRNGRSRFIHHLLSQVIFPEANLAGLDRRERTRIHWGQRALYVGSLALLLLFGLLWAGGFSANHERLERVRE
LAQQWAQQRQALGERDDAMAALKTLDSSYAATTVFPPKGEVSLHERAGLYQGNEVNPPLAAAYRRELETQLLPRVARMLE
AQIRGNMQNRERLLNSLRAYLMLNLRERRENGWLKDWVATDWSQRYAGNGTVQNGLNTHFARLLEQPFSYPLNETLVAQA
RQVLRSESLANVVYRVLREQARSLPEYRFGQHLGPQAGLFVGTDYVIPGFYTQQGYQQYFVVQGASLVNDILRDNWVLGE
GSGISDMDLRRLMVELEQLYFRDYANFWSEAVGRLGLLPFNDAGEGADQVSGLLAANSPILQLLLEVRENTRFPVLAESA
EALAEASDKAAEKGGKLGKAAAAAAGKARDALAKNLPDTAKKSLQRRFEPLHRLLDDNNGPAADLTPLLQALNELQLQLA
GLARASQPEQAAFDMAKSRMGGQRDALSNLRNASARLPRPLGGWFSVLAEDAWRLVLGDAYLYLNQRYQSELYGFYSKAI
NKRYPFNAHSASDVAVSDFREFFKAQGVAERFFETYMRPFVSGDPGSYRLRSVDGYSLPMSKVYLDQMGTAQVIRQSFFA
ENPAEPQVRFRLEPYTLDPGVSRAEFRFDGQSMEYRHGPIVPMSFKWPSDADGGRTSLVLEKMAGRPVGIERNTGPWSLF
RLFDLMQTEYLSGRDVMVLKADLGGLRANYLLLAQRTPNPFDLAVLRSFRMPVQL*

  Nucleotide sequence: 3528 bp    

>T6CP015333 NZ_CP020659:1134277-1137804 [Pseudomonas aeruginosa PAK] [TssM]
ATGAAAAACTTCTTCAAGAAAGTCGGCGCCTTCCTGCGCAAGACGTGGGTCTGGACGCTGTTGCTGGTACTCCTGGCGGC
ATTGCTGGTGTGGTTCGTCGGGCCGCTGCTGGCGGTCGCCGACTACAAGTTCTGGGAAAGCCCGACCTCGCGGTTGCTGA
GCATCAGCCTGATGTTCCTGGTCTGGGGGCTGGCGATGGTCTTCGTCAGTTGGCGCGCCGGAGTGCGGCGCAAGGCCGTC
GAGGACAGCGACGAGGGCCAGGAGCGCCTGCGCCGCGACAGTCTGATCGAAGACGAACAGAAGGAATTGCGCAGCCGCTT
CAAGGATGCCTTGCGCACCCTGAAGACCTCCAGCCTGTACCGCGGCCGCAGCGAGCGCTGGCGCAACGAGCTGCCCTGGT
ACCTGCTGATCGGCCCGCAGGGAAGCGGCAAGACCAGCCTGCTGGACTTTTCCGGACTGGAGTTCCCGATCAACCGGATC
GACCGCAAGCTGACCCGCGATACCGCCGGCACGCGCTACTGCGACTGGTACTTCGCCGACCACGGCGTCCTGATCGACAC
CGCCGGGCGCTACCTGACCCAAGCCGACGGCGAGGTCGACGGGGGCGCCTGGGCGACCCTGCTCGGCCTGTTGCGCAAGC
GCCGCCGCGCACGGCCGCTGAATGGCGTGATGGTGTCGATTCCGGTGGAAACGTTGCTCAGCGACGACGAGATGGCGCTG
GATACGCTGGCTCGCCAGGTGCGCGCGCGCCTGCAGGAAATCCAGCAGACCCTGCACCTCGATCTGCCGGTCTACCTGGT
GCTGAGCAAGGCCGACCAGTTGCTCGGCTTCGACGAGTTCTTCGACCAGTTGAGCCGCGAGGAGAGCGACCAGGTTCTGG
GCACCAGCTTCGGCAAGGAGCAGAACGGCACAGATGTGACGGTCCTGCGCCAGGCGTTCGAGGACCTTCTGCGACGCCTG
AACAGCCAGGTGATCATGCGCATCCACCAGGAGCGCGATACCCTGCGTCGCGGTCGCATCCTCGACTTCCCGCACCAGCT
TGGCCAGATCGGCGAGCGGTTGTGCCTGTTCGTCGAACTCGCCTTCACCGGCAACCGCTACCAGCGCGCCAGCCGCCTGC
GCGGCTTCTACCTGACCAGCGCGCCGCACCTGGCCCAGCAGCTGGACGAGGACACCGCCGGCATCGGCGCCAACCTGGGG
ATCGCCAGCGGCCTGCTGCCGACCCTGCGCAACGGGCGCTCGCGCTTCATCCATCACCTGCTGAGCCAGGTGATCTTCCC
CGAGGCCAACCTGGCAGGTCTCGATCGCCGCGAACGCACGCGCATCCACTGGGGCCAGCGCGCCCTGTACGTCGGTTCGC
TGGCGCTCCTTCTGCTATTCGGCCTGCTCTGGGCTGGCGGTTTCTCGGCCAACCACGAGCGCCTGGAGCGGGTCCGCGAG
CTGGCGCAGCAATGGGCCCAGCAGCGCCAGGCGCTTGGCGAGCGCGACGACGCCATGGCGGCGCTGAAGACGCTGGATAG
CAGCTATGCGGCGACCACCGTGTTTCCGCCCAAAGGGGAAGTCTCGCTGCACGAGCGCGCCGGGCTGTACCAGGGCAACG
AGGTCAACCCGCCGCTGGCCGCTGCCTATCGCCGCGAGCTGGAGACGCAACTGCTGCCGCGCGTGGCGCGGATGCTCGAA
GCGCAGATCCGCGGCAACATGCAGAATCGCGAACGCCTGCTCAACAGCCTGCGGGCCTACCTGATGCTGAATCTGCGCGA
GCGCCGCGAGAACGGATGGCTCAAGGACTGGGTGGCTACCGACTGGTCGCAGCGCTATGCCGGCAACGGCACCGTGCAGA
ATGGCCTGAACACGCATTTCGCCCGTCTGCTCGAGCAACCGTTCAGCTATCCGCTGAACGAGACCCTGGTGGCCCAGGCA
CGCCAGGTGCTGCGCAGCGAGTCCTTGGCCAACGTGGTCTACCGGGTGCTGCGCGAACAGGCGCGAAGCCTGCCCGAGTA
TCGCTTCGGCCAGCACCTGGGGCCGCAGGCCGGTCTGTTCGTCGGCACCGACTACGTGATCCCGGGGTTCTACACCCAGC
AGGGCTACCAGCAGTACTTCGTGGTCCAGGGCGCCAGCCTGGTCAACGACATCCTTCGCGACAACTGGGTCCTGGGCGAG
GGCAGCGGGATCAGCGACATGGACTTGCGGCGCCTGATGGTCGAACTCGAGCAGTTGTATTTCCGCGACTACGCGAACTT
CTGGAGCGAGGCGGTCGGACGCCTGGGCCTGCTGCCGTTCAACGATGCCGGCGAGGGCGCCGACCAGGTTTCCGGGCTGC
TCGCCGCCAACTCGCCGATCCTCCAGTTGTTGCTGGAGGTCCGTGAGAACACCCGTTTCCCGGTCCTGGCGGAGAGCGCC
GAGGCGCTGGCAGAGGCCAGCGACAAGGCCGCGGAAAAGGGCGGCAAGCTGGGCAAGGCGGCCGCCGCGGCGGCCGGCAA
GGCCAGGGACGCGTTGGCGAAGAACCTGCCGGATACCGCGAAGAAGTCCCTGCAACGCCGCTTCGAGCCCCTGCATCGCC
TGCTCGACGATAACAACGGCCCGGCGGCCGACCTCACCCCGCTGTTGCAGGCGCTCAACGAGTTGCAGTTGCAACTCGCC
GGACTGGCGCGCGCCAGCCAGCCGGAGCAGGCGGCTTTCGACATGGCGAAGAGCCGCATGGGCGGCCAGCGCGATGCCCT
GAGCAACCTGCGCAACGCCTCCGCGCGACTGCCGCGACCGCTCGGCGGATGGTTCAGCGTGCTGGCCGAAGACGCCTGGC
GGCTGGTCCTCGGCGATGCCTACCTGTACCTCAACCAGCGCTACCAGAGCGAGCTGTACGGTTTCTACAGCAAGGCGATC
AACAAGCGCTACCCGTTCAACGCGCACAGCGCCAGCGATGTCGCGGTCAGCGATTTCCGCGAGTTCTTCAAGGCGCAGGG
CGTCGCCGAACGCTTCTTCGAAACCTACATGCGGCCCTTCGTCAGCGGCGACCCGGGCAGTTACCGCCTGCGCAGCGTGG
ACGGTTACAGCCTGCCGATGTCCAAGGTCTATCTCGACCAGATGGGCACGGCCCAGGTGATCCGCCAGAGCTTCTTCGCC
GAGAACCCGGCCGAGCCACAGGTGCGCTTCCGCCTGGAGCCCTATACCCTCGATCCTGGCGTCAGCCGCGCCGAGTTCCG
TTTCGACGGTCAGTCCATGGAGTACCGCCACGGCCCGATCGTGCCGATGTCGTTCAAGTGGCCCAGCGACGCCGACGGCG
GCCGCACCAGCCTGGTGCTGGAGAAGATGGCCGGACGACCGGTGGGAATCGAACGCAATACCGGGCCCTGGTCGCTGTTC
AGGCTGTTCGACCTGATGCAGACCGAGTACCTCAGCGGGCGTGACGTGATGGTGCTCAAGGCCGACCTGGGCGGCCTGCG
CGCCAACTACCTGCTGCTCGCCCAGCGCACGCCGAATCCGTTCGATCTCGCGGTCCTGCGCAGCTTCCGCATGCCGGTGC
AGCTCTGA