Detailed information of component protein


Summary

Component ID T6CP015329
Component protein type TssH
T6SS ID (Type) T6SS01211 experimental (Type i1)
Strain Pseudomonas aeruginosa PAK
Replicon chromosome
Sequence Protein sequence (878 a.a.); Nucleotide sequence (2634 bp)
Reference
[1] Chen R, Weng Y, Zhu F, et al. Polynucleotide Phosphorylase Regulates Multiple Virulence Factors and the Stabilities of Small RNAs RsmY/Z in Pseudomonas aeruginosa. Front Microbiol. 2016 Mar 2;7:247. doi: 10.3389/fmicb.2016.00247. eCollection 2016. PMID: 26973625
[2] Li K, Xu C, Jin Y, et al. SuhB is a regulator of multiple virulence genes and essential for pathogenesis of Pseudomonas aeruginosa. mBio. 2013 Oct 29;4(6):e00419-13. doi: 10.1128/mBio.00419-13. PMID: 24169572
[3] Brencic A, Lory S. Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA. Mol Microbiol. 2009 May;72(3):612-32. doi: 10.1111/j.1365-2958.2009.06670.x. PMID: 19426209
[4] Marden JN, Diaz MR, Walton WG, et al. An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):15055-60. doi: 10.1073/pnas.1307217110. Epub 2013 Aug 26. PMID: 23980177
[5] Aragon IM, Pérez-Mendoza D, Moscoso JA, et al. Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections. Environ Microbiol. 2015 Nov;17(11):4332-51. doi: 10.1111/1462-2920.12856. Epub 2015 Apr 28. PMID: 25809128
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) Y880_RS05370
Coordinate (Strand) 1125954..1128587 (+)
NCBI ID WP_079276196.1
RefSeq NZ_CP020659
Uniprot ID UPI0009A1BA29
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 5.6e-42 611..776
AAA_lid_9 PF17871.3 6.9e-29 349..441
ClpB_D2-small PF10431.11 6.6e-11 787..857
AAA PF00004.31 8.4e-10 210..341
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-877 MSMINVDLQQLIQALDADTRRDLEDSAERCVARGGSKVLVEDLLLGLLERPQGLLARALQDAAVDAGELNAALQARGEHSASRNPVFAPELVQWLQDALLLANLELGRSQIDQAALILALLRNPLRYAGSRYQPLLARLDIERLKDFALSQPLEGAPSGKPGGGESLLQRFTHNLTQQARDGKLDPVLCRDGAIRQMVDILARRRKNNPIVVGEAGVGKTAIVEGLASRIAAGEVPPALRDVELLSLDMGLLQAGASVKGEFERRLKGVIDEVKAALRPTILFIDEAHTLIGAGGQAGGSDAANLLKPALARGELRTIAATTWAEYKKYFEKDPALARRFQPVQLHEPSVAEAVTILRGLARVYEESHGIYLRDDAVVAAAELSARYLAGRQLPDKAVDVLDTACARVRISLAAAPESLERLRGELAEGERQRQALRRDAEAGLPIDHDTLQALEERLAQAESERHVQEDQWSRQRELAERLLALRQQLARAREANREEQGGEADAETFPADEHGGVEALEKALHETRQALADAQAQGRLVSFEVCPRLVAEVIGAWTGVPVEQLAREHNAKVMGFADDLRARIRGQEQAVSALDRAMRATAAGLNKPDAPVGVFLLVGPSGVGKTETALALADLLYGGERFITTINMSEFQEKHTVSRLIGAPPGYVGYGEGGMLTEAVRQKPYSVILLDEVEKADPDVLNLFYQIFDKGVANDGEGREIDFRNTLILMTSNLASEHIAALCQDGARPSAELLEETIRPALSRHFKPALLARMRVVPYYPVGGPVLRELIEIKLERLGERLARRQLAFDYSQALVDHLAERCTQSDSGARLIDHLLDTHLLPLVADRLLEAMARDERLERVHATLGEHGRVLCEFA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9963 - -


  Protein sequence: 878 a.a.    

>T6CP015329 NZ_CP020659:1125954-1128587 [Pseudomonas aeruginosa PAK] [TssH]
MSMINVDLQQLIQALDADTRRDLEDSAERCVARGGSKVLVEDLLLGLLERPQGLLARALQDAAVDAGELNAALQARGEHS
ASRNPVFAPELVQWLQDALLLANLELGRSQIDQAALILALLRNPLRYAGSRYQPLLARLDIERLKDFALSQPLEGAPSGK
PGGGESLLQRFTHNLTQQARDGKLDPVLCRDGAIRQMVDILARRRKNNPIVVGEAGVGKTAIVEGLASRIAAGEVPPALR
DVELLSLDMGLLQAGASVKGEFERRLKGVIDEVKAALRPTILFIDEAHTLIGAGGQAGGSDAANLLKPALARGELRTIAA
TTWAEYKKYFEKDPALARRFQPVQLHEPSVAEAVTILRGLARVYEESHGIYLRDDAVVAAAELSARYLAGRQLPDKAVDV
LDTACARVRISLAAAPESLERLRGELAEGERQRQALRRDAEAGLPIDHDTLQALEERLAQAESERHVQEDQWSRQRELAE
RLLALRQQLARAREANREEQGGEADAETFPADEHGGVEALEKALHETRQALADAQAQGRLVSFEVCPRLVAEVIGAWTGV
PVEQLAREHNAKVMGFADDLRARIRGQEQAVSALDRAMRATAAGLNKPDAPVGVFLLVGPSGVGKTETALALADLLYGGE
RFITTINMSEFQEKHTVSRLIGAPPGYVGYGEGGMLTEAVRQKPYSVILLDEVEKADPDVLNLFYQIFDKGVANDGEGRE
IDFRNTLILMTSNLASEHIAALCQDGARPSAELLEETIRPALSRHFKPALLARMRVVPYYPVGGPVLRELIEIKLERLGE
RLARRQLAFDYSQALVDHLAERCTQSDSGARLIDHLLDTHLLPLVADRLLEAMARDERLERVHATLGEHGRVLCEFA*

  Nucleotide sequence: 2634 bp    

>T6CP015329 NZ_CP020659:1125954-1128587 [Pseudomonas aeruginosa PAK] [TssH]
ATGAGCATGATCAACGTAGACCTGCAACAACTGATTCAGGCGCTGGATGCCGACACCCGGCGCGACCTCGAGGACTCGGC
CGAGCGCTGCGTAGCCCGTGGCGGCAGCAAGGTACTGGTGGAGGACCTGTTGCTGGGCCTGCTGGAGCGTCCCCAGGGAC
TGCTGGCGCGCGCCCTGCAGGATGCCGCGGTGGACGCCGGCGAACTCAACGCCGCGTTGCAAGCGCGGGGCGAGCATAGT
GCCTCGCGCAATCCGGTGTTCGCGCCCGAGCTGGTGCAGTGGCTGCAGGATGCGCTGTTGCTGGCCAACCTCGAACTGGG
CCGTAGCCAGATCGACCAGGCGGCATTGATCCTGGCCCTGCTACGCAACCCGTTGCGCTATGCCGGCAGCCGCTACCAGC
CGCTGCTGGCGCGGCTCGACATCGAGCGGTTGAAGGATTTCGCGCTCTCGCAGCCGCTGGAGGGCGCGCCCAGCGGCAAG
CCGGGCGGCGGCGAGTCCCTGTTGCAGCGTTTCACCCACAACCTTACCCAGCAGGCGCGCGACGGCAAGCTCGATCCGGT
GCTCTGTCGCGACGGGGCTATTCGCCAGATGGTCGACATCCTGGCCCGTCGGCGGAAAAACAACCCCATCGTGGTCGGCG
AGGCCGGCGTCGGCAAGACCGCCATCGTCGAAGGCCTGGCCTCGCGGATCGCCGCAGGGGAAGTGCCGCCGGCGCTGAGG
GACGTCGAGCTGTTGTCCCTCGACATGGGCCTGTTGCAGGCCGGTGCCAGCGTGAAGGGCGAGTTCGAGCGGCGCCTGAA
AGGCGTCATCGACGAAGTGAAGGCAGCGCTCAGGCCGACCATCCTGTTCATCGACGAGGCCCATACGCTGATCGGCGCGG
GCGGTCAGGCCGGCGGTTCGGATGCCGCCAACCTGCTAAAGCCGGCCCTGGCACGTGGCGAGTTGCGCACCATCGCGGCG
ACGACCTGGGCCGAATACAAGAAGTATTTCGAGAAGGACCCGGCGCTGGCCCGGCGCTTCCAGCCCGTGCAGTTGCATGA
GCCGAGCGTTGCCGAGGCGGTGACGATCCTGCGTGGCCTGGCCCGGGTTTACGAGGAAAGCCACGGTATCTACCTGCGCG
ACGACGCGGTGGTGGCCGCCGCCGAACTGTCCGCCCGCTACCTGGCGGGTCGCCAGTTGCCGGACAAGGCCGTCGACGTC
CTCGACACCGCCTGTGCGCGGGTGCGCATCAGCCTGGCGGCGGCGCCGGAGAGTCTCGAGCGCCTGCGCGGCGAGCTGGC
CGAAGGCGAGCGCCAGCGCCAGGCCCTGCGCCGCGATGCCGAGGCAGGCCTGCCGATCGACCACGATACCCTGCAGGCGC
TGGAGGAGCGCCTGGCCCAGGCGGAAAGCGAGCGGCACGTACAGGAGGACCAGTGGAGCCGCCAGCGCGAACTCGCCGAG
CGCCTGCTCGCCCTGCGCCAGCAATTGGCCAGGGCCCGCGAGGCGAACCGGGAAGAGCAGGGCGGCGAGGCGGACGCGGA
GACTTTCCCGGCCGACGAGCATGGCGGTGTCGAAGCCCTGGAAAAGGCCCTTCACGAAACCCGCCAGGCGCTCGCCGATG
CCCAGGCCCAGGGGCGGCTGGTCAGCTTCGAGGTCTGCCCGCGCCTGGTTGCCGAGGTGATCGGCGCCTGGACCGGCGTA
CCGGTGGAACAACTGGCGCGGGAACACAACGCCAAGGTGATGGGGTTCGCTGACGACCTGCGGGCGCGGATCCGTGGCCA
GGAACAGGCGGTCAGCGCCCTGGACCGCGCGATGCGCGCCACCGCGGCCGGGCTGAACAAGCCCGATGCGCCGGTCGGCG
TGTTCCTGCTGGTGGGCCCGAGCGGGGTGGGCAAGACCGAGACCGCGCTGGCGCTGGCCGACCTGCTGTATGGCGGCGAG
CGTTTCATCACCACCATCAACATGTCCGAGTTCCAGGAGAAGCACACCGTCTCGCGCCTGATCGGCGCGCCGCCCGGCTA
TGTCGGCTATGGCGAGGGTGGCATGCTCACCGAGGCGGTACGCCAGAAGCCCTATTCGGTGATATTGCTGGACGAGGTGG
AGAAGGCCGATCCGGACGTGCTCAACCTGTTCTACCAGATCTTCGACAAGGGCGTGGCCAACGATGGCGAAGGGCGCGAG
ATCGATTTCCGCAATACCTTGATCCTGATGACCTCCAACCTCGCCAGCGAGCACATCGCCGCGCTTTGCCAGGACGGCGC
GCGGCCTTCCGCCGAACTCCTGGAGGAAACCATCCGGCCGGCGCTCAGCCGGCATTTCAAGCCGGCGCTGCTGGCGCGCA
TGCGCGTGGTGCCTTACTACCCGGTGGGCGGCCCGGTACTGCGCGAGCTGATCGAGATCAAGCTGGAACGCCTCGGCGAG
CGTCTGGCCCGCCGTCAACTGGCTTTCGACTACAGCCAGGCGCTGGTGGACCACCTGGCCGAGCGTTGCACCCAGAGCGA
CAGCGGCGCACGGTTGATCGACCACCTGCTGGACACGCACCTGCTGCCGCTGGTGGCCGACCGTTTGCTGGAAGCGATGG
CGCGCGACGAGCGTCTCGAGCGGGTCCATGCGACGCTGGGCGAGCACGGCCGCGTGCTCTGCGAGTTCGCCTGA