Detailed information of component protein


Summary

Component ID T6CP012524
Component protein type TagF
T6SS ID (Type) T6SS00540 experimental (Type i3)
Strain Pseudomonas fluorescens F113
Replicon chromosome
Sequence Protein sequence (218 a.a.); Nucleotide sequence (654 bp)
Reference
[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[2] Redondo-Nieto M, Barret M, Morrissey J, et al. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics. 2013 Jan 25;14:54. doi: 10.1186/1471-2164-14-54. PMID: 23350846
[3] Durán D, Bernal P, Vazquez-Arias D, et al. Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome. Sci Rep. 2021 Mar 11;11(1):5772. doi: 10.1038/s41598-021-85218-1. PMID: 33707614
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSF113_RS59785
Coordinate (Strand) 6671736..6672389 (-)
NCBI ID WP_014340879.1
RefSeq NC_016830
Uniprot ID G8Q709_PSEF3
KEGG ID pfe:PSF113_5791
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
TagF_N PF09867.11 1.4e-47 5..139
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-217 MSTPGFYGKLASRGDFVSRGLPQSFIGPWDSWLAAGLLASQTNLGERWLDAYLVSPLWRFMVAPGVCGPDAAVGVVMPSIDRVGRYFPLTVAMLLEPDADPASVVGGADEWFERVENLLLSTLSVEASFEAFNEQLETLGSPMYLPRTPSSRFASLHRFDATDPQRRMSALAEAACEGASLWWGQGSERIAPGLMRCQGLPAAADFAQFLLGQEGVV


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9625 - -


  Protein sequence: 218 a.a.    

>T6CP012524 NC_016830:c6672389-6671736 [Pseudomonas fluorescens F113] [TagF]
MSTPGFYGKLASRGDFVSRGLPQSFIGPWDSWLAAGLLASQTNLGERWLDAYLVSPLWRFMVAPGVCGPDAAVGVVMPSI
DRVGRYFPLTVAMLLEPDADPASVVGGADEWFERVENLLLSTLSVEASFEAFNEQLETLGSPMYLPRTPSSRFASLHRFD
ATDPQRRMSALAEAACEGASLWWGQGSERIAPGLMRCQGLPAAADFAQFLLGQEGVV*

  Nucleotide sequence: 654 bp    

>T6CP012524 NC_016830:c6672389-6671736 [Pseudomonas fluorescens F113] [TagF]
ATGAGTACACCGGGCTTCTATGGAAAGTTGGCCAGCCGCGGCGATTTCGTCAGCCGTGGCTTGCCCCAGAGTTTTATCGG
CCCGTGGGATTCGTGGTTGGCGGCGGGTTTGCTCGCCAGCCAGACCAACCTTGGCGAGCGCTGGCTGGATGCCTATCTGG
TCAGCCCGCTGTGGCGCTTCATGGTCGCGCCGGGAGTGTGTGGCCCGGACGCCGCGGTCGGCGTGGTGATGCCGAGCATC
GACCGGGTCGGTCGTTATTTTCCGCTGACCGTCGCGATGCTGCTGGAGCCTGACGCGGACCCGGCGTCGGTGGTGGGCGG
TGCGGACGAGTGGTTTGAGCGAGTCGAGAACCTGTTGCTGAGCACGTTGAGTGTGGAGGCCAGTTTCGAGGCGTTCAACG
AACAGCTGGAGACCCTGGGCAGCCCGATGTACCTGCCGCGCACGCCGAGCAGCCGCTTCGCCAGCCTGCATCGCTTCGAT
GCCACCGACCCGCAGCGGCGCATGAGCGCCCTCGCCGAGGCGGCCTGCGAAGGCGCGAGCCTGTGGTGGGGCCAGGGATC
GGAACGCATTGCCCCGGGTTTGATGCGCTGCCAGGGATTGCCGGCGGCGGCTGATTTTGCGCAATTTTTGCTCGGCCAAG
AAGGTGTTGTGTAG