Detailed information of component protein


Summary

Component ID T6CP012523
Component protein type PppA
T6SS ID (Type) T6SS00540 experimental (Type i3)
Strain Pseudomonas fluorescens F113
Replicon chromosome
Sequence Protein sequence (244 a.a.); Nucleotide sequence (732 bp)
Reference
[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[2] Redondo-Nieto M, Barret M, Morrissey J, et al. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics. 2013 Jan 25;14:54. doi: 10.1186/1471-2164-14-54. PMID: 23350846
[3] Durán D, Bernal P, Vazquez-Arias D, et al. Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome. Sci Rep. 2021 Mar 11;11(1):5772. doi: 10.1038/s41598-021-85218-1. PMID: 33707614
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSF113_RS59780
Coordinate (Strand) 6671004..6671735 (-)
NCBI ID WP_003206909.1
RefSeq NC_016830
Uniprot ID I4KLX4_PSEFL, A0A2M8STJ0_9PSED, A0A176NJV6_9PSED, F2K703_PSEBN
KEGG ID pba:PSEBR_a5550
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
PP2C_2 PF13672.8 5.4e-08 21..204
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-243 MRPSAGKAIKSASKSHVGMVRQVNEDACLDLPENGLWVVADGMGGHAAGDYVSSLIVDSLRGIAVGRSLDEYVAALQSDLLRVNAAVREETANRGVTMMGSTVVVLAARDLRGMCLWAGDSRLYRLRDGVLEGISRDHSYVQDLQDSGLLSEAEARVHPRANIVTRAIGVEAQLNLALTELLLVPGDSYLLCSDGLTKTVEDDEIREVLSHDEPGEIASSLVSLGLMRGAPDNITVVVVKVPS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9708 - -


  Protein sequence: 244 a.a.    

>T6CP012523 NC_016830:c6671735-6671004 [Pseudomonas fluorescens F113] [PppA]
MRPSAGKAIKSASKSHVGMVRQVNEDACLDLPENGLWVVADGMGGHAAGDYVSSLIVDSLRGIAVGRSLDEYVAALQSDL
LRVNAAVREETANRGVTMMGSTVVVLAARDLRGMCLWAGDSRLYRLRDGVLEGISRDHSYVQDLQDSGLLSEAEARVHPR
ANIVTRAIGVEAQLNLALTELLLVPGDSYLLCSDGLTKTVEDDEIREVLSHDEPGEIASSLVSLGLMRGAPDNITVVVVK
VPS*

  Nucleotide sequence: 732 bp    

>T6CP012523 NC_016830:c6671735-6671004 [Pseudomonas fluorescens F113] [PppA]
ATGCGTCCAAGTGCCGGAAAAGCAATCAAGTCTGCAAGCAAGAGCCATGTCGGGATGGTCCGCCAGGTCAACGAAGACGC
CTGCCTGGACCTGCCGGAAAACGGCCTGTGGGTGGTGGCCGACGGCATGGGCGGGCACGCGGCGGGCGACTACGTCAGCA
GCCTGATCGTCGACAGCCTGCGCGGCATTGCCGTGGGTCGTTCTCTGGACGAATACGTCGCGGCGCTGCAAAGCGACCTG
CTGCGGGTCAACGCCGCCGTGCGGGAAGAAACCGCCAACCGTGGCGTGACCATGATGGGCAGCACCGTGGTGGTGTTGGC
CGCCCGCGACCTGCGCGGCATGTGCCTGTGGGCCGGTGACAGCCGTTTGTATCGCCTGCGCGACGGCGTGCTCGAAGGCA
TCTCCCGCGACCACAGCTACGTCCAGGATCTGCAGGACAGCGGCCTGCTCAGCGAAGCCGAGGCGCGGGTGCATCCACGG
GCCAACATCGTCACCCGGGCCATTGGCGTCGAGGCGCAACTGAACCTGGCGCTGACCGAGCTGCTGCTGGTGCCCGGCGA
CAGTTACCTGTTGTGCAGCGACGGGCTGACCAAGACCGTCGAGGACGATGAAATCCGCGAGGTATTGAGCCACGACGAGC
CCGGCGAAATCGCCAGCAGCCTGGTGTCCCTGGGCCTGATGCGCGGCGCGCCGGACAACATCACCGTGGTGGTCGTGAAG
GTGCCGTCATGA