Detailed information of component protein


Summary

Component ID T6CP012436
Component protein type Stk1
T6SS ID (Type) T6SS00042 experimental (Type i1)
Strain Pseudomonas syringae pv. tomato str. DC3000
Replicon chromosome
Sequence Protein sequence (336 a.a.); Nucleotide sequence (1008 bp)
Reference
[1] Haapalainen M, Mosorin H, Dorati F, et al. Hcp2, a secreted protein of the phytopathogen Pseudomonas syringae pv. tomato DC3000, is required for fitness for competition against bacteria and yeasts. J Bacteriol. 2012 Sep;194(18):4810-22. doi: 10.1128/JB.00611-12. Epub 2012 Jun 29. PMID: 22753062
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSPTO_RS28020
Coordinate (Strand) 6155611..6156618 (-)
NCBI ID WP_046464012.1
RefSeq NC_004578
Uniprot ID A0A0Q0HFK4_PSEUB
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Pkinase PF00069.27 2.3e-37 54..314
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-335 MTELVRPNDDLLVNEEQDSNLTYFAFAKSHKAEAALAPTKASIGEMPDVLAGRYRIERLLGAGGMGAVYRAKDLLSEQFGEPDPYVALKILSEEFAESPDASALLYSEFALTRRLRHNNVLRLHSFEVDTNCQRAFITMELMRGLTLDKLLCERPLGLPWRELRDIALSLLDALAYSHARGVLHGDMKPSNVMLSEEGVRLFDFGLGQAEEGVMPGLPHLSRERFNAWTPGYAAPELLEGQALSASADVYGVACVIFELAGGKHPFRRLPSTQARDERLERELKAPRNLPKHCWPALRSALRFDAQDRKITAKQLRDAFAVTSSRLQRLMPWCNG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9787 - -


  Protein sequence: 336 a.a.    

>T6CP012436 NC_004578:c6156618-6155611 [Pseudomonas syringae pv. tomato str. DC3000] [Stk1]
MTELVRPNDDLLVNEEQDSNLTYFAFAKSHKAEAALAPTKASIGEMPDVLAGRYRIERLLGAGGMGAVYRAKDLLSEQFG
EPDPYVALKILSEEFAESPDASALLYSEFALTRRLRHNNVLRLHSFEVDTNCQRAFITMELMRGLTLDKLLCERPLGLPW
RELRDIALSLLDALAYSHARGVLHGDMKPSNVMLSEEGVRLFDFGLGQAEEGVMPGLPHLSRERFNAWTPGYAAPELLEG
QALSASADVYGVACVIFELAGGKHPFRRLPSTQARDERLERELKAPRNLPKHCWPALRSALRFDAQDRKITAKQLRDAFA
VTSSRLQRLMPWCNG*

  Nucleotide sequence: 1008 bp    

>T6CP012436 NC_004578:c6156618-6155611 [Pseudomonas syringae pv. tomato str. DC3000] [Stk1]
ATGACTGAGCTCGTGCGCCCGAACGACGACCTGCTGGTGAACGAAGAGCAGGACAGCAACCTGACCTACTTTGCGTTTGC
CAAGTCACACAAGGCAGAAGCTGCACTGGCGCCGACCAAGGCCAGTATTGGTGAGATGCCCGACGTGTTGGCAGGTCGTT
ACCGTATCGAGCGACTGCTCGGCGCCGGTGGCATGGGCGCTGTCTACCGGGCCAAGGATTTGCTGAGCGAACAGTTCGGC
GAGCCCGATCCTTATGTGGCGCTGAAAATCCTCAGCGAAGAATTTGCCGAGTCGCCGGACGCAAGCGCCTTGCTTTACAG
CGAGTTCGCCCTGACCCGACGCCTTCGTCACAATAACGTGCTGCGTTTGCACTCGTTCGAAGTGGACACTAACTGCCAGC
GGGCTTTCATCACCATGGAACTCATGCGTGGGCTGACTTTGGACAAACTGCTATGCGAACGGCCTTTGGGCCTGCCGTGG
AGAGAACTGCGTGACATTGCATTGTCGTTGCTCGACGCACTGGCTTATTCACATGCTCGCGGCGTGTTGCATGGCGACAT
GAAACCCAGCAATGTCATGCTCAGCGAAGAAGGTGTGCGCCTGTTCGACTTTGGCCTTGGCCAGGCCGAGGAAGGCGTAA
TGCCGGGGCTGCCTCACTTGAGTCGCGAACGTTTCAACGCTTGGACTCCGGGCTACGCAGCCCCGGAATTGCTGGAAGGC
CAGGCACTATCAGCCAGCGCCGATGTGTATGGCGTTGCCTGTGTAATTTTTGAACTGGCAGGAGGCAAGCACCCGTTTCG
CCGCTTGCCGTCGACCCAGGCGCGTGACGAGCGTCTTGAGCGTGAGCTGAAGGCACCAAGGAATTTACCGAAACACTGCT
GGCCTGCTTTGCGGTCAGCACTGAGATTCGATGCACAGGATCGCAAGATCACTGCCAAACAACTGCGTGACGCTTTCGCC
GTCACTTCGTCCAGGCTGCAACGCTTGATGCCGTGGTGTAACGGATGA