Detailed information of component protein


Summary

Component ID T6CP011977
Component protein type FHA
T6SS ID (Type) T6SS00540 experimental (Type i3)
Strain Pseudomonas fluorescens F113
Replicon chromosome
Sequence Protein sequence (504 a.a.); Nucleotide sequence (1512 bp)
Reference
[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[2] Redondo-Nieto M, Barret M, Morrissey J, et al. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics. 2013 Jan 25;14:54. doi: 10.1186/1471-2164-14-54. PMID: 23350846
[3] DurĂ¡n D, Bernal P, Vazquez-Arias D, et al. Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome. Sci Rep. 2021 Mar 11;11(1):5772. doi: 10.1038/s41598-021-85218-1. PMID: 33707614
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) PSF113_RS59810
Coordinate (Strand) 6679125..6680636 (-)
NCBI ID WP_041476202.1
RefSeq NC_016830
Uniprot ID UPI00059E317F
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
FHA PF00498.28 5.5e-16 30..97

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-503 MPLCLTITSYHKITPGQCSEKSMDQGVMAIGRNSDNDWVLPDPERLVSGKHCVIQYKDGRYYLTDNSTNGVELVKAGVRLRKGNSEPLQDGEVIRIGDYEIQARVDFSLPVTDSNPFAESPSSFEALMGRQGAAVHTPSPIQVTSPAHFQGGSAMDTLPDLFDFLSPSSVPPATQPDHVPAEQHDFRPPTPIPRPSPPPVVEPVMPPSASVIPDDWDPFSDKPAPVAVAPLPVAPPPVPLPPVLEPVATPAPQAPLPDIFAEIIAPPPPVPEASVTRVEPPVAPPVAPVAPVAPVAPVVPVVPVEANQPDLLQAFLRGAGLDQLRLDKANAEAQMESIGRSYRLMVEGLIDVLRARSSLKGEFRIQQTMIQPVENNPLKFAPNVDEALLLLLRHGNQAFMAPDAAVRDSFDDLRAHQLAVMAGVEAAIKHLLGRFEPAQLEERMAKPGGLSGIFSGSRQAQYWQQFTELYSNISREAQEDFQDLFGREFSRAYEEHSTRQRRR



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8427 - -



  Protein sequence: 504 a.a.    

>T6CP011977 NC_016830:c6680636-6679125 [Pseudomonas fluorescens F113] [FHA]
MPLCLTITSYHKITPGQCSEKSMDQGVMAIGRNSDNDWVLPDPERLVSGKHCVIQYKDGRYYLTDNSTNGVELVKAGVRL
RKGNSEPLQDGEVIRIGDYEIQARVDFSLPVTDSNPFAESPSSFEALMGRQGAAVHTPSPIQVTSPAHFQGGSAMDTLPD
LFDFLSPSSVPPATQPDHVPAEQHDFRPPTPIPRPSPPPVVEPVMPPSASVIPDDWDPFSDKPAPVAVAPLPVAPPPVPL
PPVLEPVATPAPQAPLPDIFAEIIAPPPPVPEASVTRVEPPVAPPVAPVAPVAPVAPVVPVVPVEANQPDLLQAFLRGAG
LDQLRLDKANAEAQMESIGRSYRLMVEGLIDVLRARSSLKGEFRIQQTMIQPVENNPLKFAPNVDEALLLLLRHGNQAFM
APDAAVRDSFDDLRAHQLAVMAGVEAAIKHLLGRFEPAQLEERMAKPGGLSGIFSGSRQAQYWQQFTELYSNISREAQED
FQDLFGREFSRAYEEHSTRQRRR*

  Nucleotide sequence: 1512 bp    

>T6CP011977 NC_016830:c6680636-6679125 [Pseudomonas fluorescens F113] [FHA]
ATGCCGCTGTGTTTGACTATCACTAGTTATCACAAGATTACCCCAGGCCAGTGTTCTGAAAAGTCCATGGATCAGGGGGT
GATGGCAATTGGCCGTAATTCCGATAATGACTGGGTATTGCCGGACCCTGAGCGTCTGGTTTCCGGCAAACATTGCGTTA
TCCAATACAAAGATGGGCGTTATTACTTAACCGATAACAGCACTAATGGTGTGGAATTGGTCAAGGCCGGTGTTCGCCTG
CGCAAGGGCAACAGCGAACCCCTGCAAGATGGCGAAGTGATCCGCATCGGTGATTATGAAATCCAGGCGCGCGTCGATTT
CAGCCTGCCGGTCACCGACAGCAACCCGTTTGCCGAATCGCCCAGCAGTTTCGAGGCCTTGATGGGGCGCCAGGGCGCAG
CGGTGCATACACCGTCGCCGATCCAAGTGACGTCGCCTGCGCATTTCCAGGGTGGGTCGGCCATGGACACCTTGCCGGAT
CTGTTCGATTTCTTGAGTCCGAGCAGTGTCCCGCCCGCCACCCAGCCCGACCATGTGCCGGCCGAGCAACACGATTTCCG
CCCGCCGACACCGATTCCCCGGCCGAGCCCGCCGCCCGTGGTGGAGCCGGTGATGCCGCCGTCTGCCTCGGTGATTCCGG
ATGACTGGGACCCGTTCAGCGACAAGCCCGCGCCAGTGGCGGTTGCCCCGCTGCCCGTGGCTCCGCCGCCTGTGCCGCTA
CCGCCGGTGCTTGAGCCGGTCGCGACGCCTGCCCCGCAGGCTCCGCTCCCCGACATCTTTGCTGAAATCATTGCACCGCC
GCCGCCCGTGCCTGAAGCGTCGGTCACTCGCGTCGAGCCCCCGGTCGCACCTCCAGTTGCTCCAGTTGCTCCAGTTGCTC
CAGTTGCTCCGGTTGTTCCAGTCGTCCCGGTCGAGGCCAACCAACCCGACTTGCTGCAAGCGTTCCTGCGCGGTGCCGGG
CTCGACCAGTTGCGCCTGGACAAGGCCAACGCCGAAGCTCAGATGGAAAGCATCGGGCGCAGCTATCGGTTGATGGTCGA
AGGCCTGATCGATGTCTTGCGCGCCCGCAGCAGCCTCAAGGGTGAGTTCCGTATCCAGCAGACGATGATCCAGCCGGTGG
AAAACAACCCGCTGAAATTCGCCCCCAATGTCGATGAAGCCCTGCTGTTGCTGTTGCGCCACGGCAACCAGGCGTTCATG
GCCCCGGACGCGGCGGTGCGTGACAGTTTCGATGACTTGCGGGCCCACCAACTGGCGGTGATGGCCGGTGTGGAAGCGGC
CATCAAGCATTTGCTGGGGCGCTTCGAGCCGGCGCAACTGGAAGAACGCATGGCCAAGCCCGGTGGGCTCTCGGGTATTT
TCAGCGGCTCGCGACAGGCCCAGTACTGGCAGCAGTTCACCGAGCTCTACAGCAACATTTCCCGGGAGGCCCAAGAGGAT
TTCCAGGACCTGTTCGGTCGCGAGTTCAGCCGCGCCTACGAAGAACACAGTACGCGACAGCGACGCCGCTAA