SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP009581
Component protein type	TssL
T6SS ID (Type)	T6SS00014 (Type i4b)
Strain	Pseudomonas putida KT2440
Replicon	chromosome
Sequence	Protein sequence (239 a.a.); Nucleotide sequence (717 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537 [3] Bernal P, Allsopp LP, Filloux A, et al. The Pseudomonas putida T6SS is a plant warden against phytopathogens. ISME J. 2017 Apr;11(4):972-987. doi: 10.1038/ismej.2016.169. Epub 2017 Jan 3. PMID: 28045455

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PP_RS16015
Coordinate (Strand)	3485954..3486670 (-)
NCBI ID	WP_010953990.1
RefSeq	NC_002947
Uniprot ID	Q88IA7_PSEPK, A0A2V4FB89_9PSED
KEGG ID	ppu:PP_3092
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	5.4e-53	20..219

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-198	MTEAVLQQGAVPAASDKPTLKDLVQDFISMALIVRRGRQVTSVQAFEGSVERFFANLERDARAANYSVEQVKDTQYALCAFLDESVLRSDDNALRRHFELQPLQFRYFGVHLAGEGFFEKVDALRADVKQNIDVLEVYHLCLALGFEGKFSLGQKDQLRYLANTLGQDIARYRKAPKALSPDWALPDQVSQMLRHEVP
Transmembrane helix	199-221	LWVYLALIALVCVAVYLTLDWLL
Outside	222-238	GKDVAALSEQIRQLFSA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9805	-	-

Protein sequence: 239 a.a.

						>T6CP009581 NC_002947:c3486670-3485954 [Pseudomonas putida KT2440] [TssL]
MTEAVLQQGAVPAASDKPTLKDLVQDFISMALIVRRGRQVTSVQAFEGSVERFFANLERDARAANYSVEQVKDTQYALCA
FLDESVLRSDDNALRRHFELQPLQFRYFGVHLAGEGFFEKVDALRADVKQNIDVLEVYHLCLALGFEGKFSLGQKDQLRY
LANTLGQDIARYRKAPKALSPDWALPDQVSQMLRHEVPLWVYLALIALVCVAVYLTLDWLLGKDVAALSEQIRQLFSA*					

Nucleotide sequence: 717 bp

						>T6CP009581 NC_002947:c3486670-3485954 [Pseudomonas putida KT2440] [TssL]
ATGACCGAAGCCGTACTGCAACAGGGCGCCGTCCCGGCCGCCAGCGACAAACCGACGCTCAAGGACCTGGTCCAGGACTT
CATCAGCATGGCGTTGATCGTACGTCGCGGCCGCCAGGTCACCTCGGTGCAGGCCTTCGAAGGTAGCGTCGAACGCTTCT
TCGCCAACCTGGAACGTGATGCCCGTGCCGCCAACTACAGCGTCGAGCAGGTCAAGGACACCCAATACGCGTTGTGCGCC
TTCCTCGACGAAAGCGTCCTGCGCTCGGACGACAATGCGCTGCGTCGGCACTTTGAGCTGCAGCCGTTGCAGTTCCGCTA
TTTCGGCGTGCACCTGGCCGGCGAAGGCTTCTTCGAGAAGGTCGATGCGCTGCGCGCCGACGTCAAGCAGAACATCGACG
TGCTCGAGGTTTACCACCTGTGCCTGGCACTGGGCTTCGAGGGCAAGTTCAGCCTCGGCCAAAAGGACCAGCTGCGTTAC
CTGGCCAATACACTGGGCCAGGATATCGCCCGCTACCGCAAGGCGCCCAAAGCCCTGTCACCGGACTGGGCACTGCCCGA
CCAGGTGTCGCAGATGCTGCGCCATGAAGTACCGCTGTGGGTGTATCTGGCGCTTATCGCGTTGGTCTGCGTCGCCGTGT
ACCTGACGCTGGACTGGTTGCTTGGCAAGGACGTCGCCGCCCTGTCCGAACAAATCCGCCAGCTGTTCAGTGCCTGA					