Detailed information of component protein


Summary

Component ID T6CP008457
Component protein type TssH
T6SS ID (Type) T6SS00040 experimental (Type i1)
Strain Pectobacterium atrosepticum SCRI1043
Replicon chromosome
Sequence Protein sequence (866 a.a.); Nucleotide sequence (2598 bp)
Reference
[1] Mattinen L, Somervuo P, Nykyri J, et al. Microarray profiling of host-extract-induced genes and characterization of the type VI secretion cluster in the potato pathogen Pectobacterium atrosepticum. Microbiology (Reading). 2008 Aug;154(Pt 8):2387-2396. doi: 10.1099/mic.0.2008/017582-0. PMID: 18667571
[2] Mattinen L, Nissinen R, Riipi T, et al. Host-extract induced changes in the secretome of the plant pathogenic bacterium Pectobacterium atrosepticum. Proteomics. 2007 Oct;7(19):3527-37. PMID: 17726675
[3] Liu H, Coulthurst SJ, Pritchard L, et al. Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum. PLoS Pathog. 2008 Jun 20;4(6):e1000093. doi: 10.1371/journal.ppat.1000093. PMID: 18566662
[4] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[5] Bernard CS, Brunet YR, Gavioli M, et al. Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. J Bacteriol. 2011 May;193(9):2158-67. doi: 10.1128/JB.00029-11. Epub 2011 Mar 4. PMID: 21378190
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ECA_RS16945
Coordinate (Strand) 3852301..3854898 (-)
NCBI ID WP_011094944.1
RefSeq NC_004547
Uniprot ID Q6D1L0_PECAS
KEGG ID eca:ECA3436
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 9.5e-37 602..763
AAA_lid_9 PF17871.3 9.3e-29 359..450
ClpB_D2-small PF10431.11 1.1e-08 769..843
AAA PF00004.31 8.8e-08 219..331
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-865 VIRIELPTLVERLNPVCRHMLEEAAALCIQHQGAEIRIEHLLMKMLETPLCDVRQILKRAGVDADELSSLLLPSSVDKEFDAGYPSFSPLLVEWLQDSWLLASAEFQHAQLRSGILLLVLLLTPNRYVAGAVSRPLAQINRELLRQQFDEWVKDSVETEVATRSATAEQAAAATTQLSRYTQNVTESARQGQLDPVLCRDHEIDLMIDILSRRRKNNPIVVGEAGVGKSALIEGLALRIVAGAVPERLRDVELLTLDLGAMQAGASVKGEFEKRFKGVMQEVKDAPRPIILFIDEAHTLIGAGNQAGGLDVSNLLKPALARGELRTIAATTWSEYKKYVEKDAALSRRFQLVKVGEPNAEEATVILRGLRSIYEKAHGVLIDEDALQAAAQLSARYISGRQLPDKAIDVLDTASARVAINLTTPPRAVSQLQTRLHQQKMEITQLERQARIGLGNTEERLVELRDAREAGAASLAQLEADWQQQKTQVQRVIELRTALLDDEQTVDFDAVSAAAELATCEQALEILQQASVLVSPHVDKMQIAAVIAEWTGVPLNRISQGEMDIVTRLPEFLGESIKGQQLAIAQLHKHLLTARADLRRPGRPLGAFLLVGPSGVGKTETVVQIADLMFGGRNYLTTINMSEYQEKHTVSRLIGSPPGYVGFGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGELADGEGRVIDCRNVVFFLTSNLGFQTIVNYAEQSDVLLDALYPELAAFFKPALLARMEVIPYLPLAHDTMVEIVQGKLSRLVSLLQQRFGAEVIIEDEVPEEILRLANRSENGARMLESVIDGALLPPVSLQLLQRMSAGEPVSRIHFRVEAGQFQTEVEG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9967 - -


  Protein sequence: 866 a.a.    

>T6CP008457 NC_004547:c3854898-3852301 [Pectobacterium atrosepticum SCRI1043] [TssH]
VIRIELPTLVERLNPVCRHMLEEAAALCIQHQGAEIRIEHLLMKMLETPLCDVRQILKRAGVDADELSSLLLPSSVDKEF
DAGYPSFSPLLVEWLQDSWLLASAEFQHAQLRSGILLLVLLLTPNRYVAGAVSRPLAQINRELLRQQFDEWVKDSVETEV
ATRSATAEQAAAATTQLSRYTQNVTESARQGQLDPVLCRDHEIDLMIDILSRRRKNNPIVVGEAGVGKSALIEGLALRIV
AGAVPERLRDVELLTLDLGAMQAGASVKGEFEKRFKGVMQEVKDAPRPIILFIDEAHTLIGAGNQAGGLDVSNLLKPALA
RGELRTIAATTWSEYKKYVEKDAALSRRFQLVKVGEPNAEEATVILRGLRSIYEKAHGVLIDEDALQAAAQLSARYISGR
QLPDKAIDVLDTASARVAINLTTPPRAVSQLQTRLHQQKMEITQLERQARIGLGNTEERLVELRDAREAGAASLAQLEAD
WQQQKTQVQRVIELRTALLDDEQTVDFDAVSAAAELATCEQALEILQQASVLVSPHVDKMQIAAVIAEWTGVPLNRISQG
EMDIVTRLPEFLGESIKGQQLAIAQLHKHLLTARADLRRPGRPLGAFLLVGPSGVGKTETVVQIADLMFGGRNYLTTINM
SEYQEKHTVSRLIGSPPGYVGFGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGELADGEGRVIDCRNVVF
FLTSNLGFQTIVNYAEQSDVLLDALYPELAAFFKPALLARMEVIPYLPLAHDTMVEIVQGKLSRLVSLLQQRFGAEVIIE
DEVPEEILRLANRSENGARMLESVIDGALLPPVSLQLLQRMSAGEPVSRIHFRVEAGQFQTEVEG*

  Nucleotide sequence: 2598 bp    

>T6CP008457 NC_004547:c3854898-3852301 [Pectobacterium atrosepticum SCRI1043] [TssH]
GTGATTCGAATTGAACTGCCGACACTGGTTGAACGACTTAACCCCGTGTGCCGTCATATGCTGGAAGAAGCGGCGGCACT
TTGTATTCAACATCAGGGAGCGGAAATCCGTATTGAGCACCTGTTGATGAAAATGCTGGAAACGCCGCTGTGTGATGTGC
GCCAGATCCTCAAGCGCGCGGGTGTCGATGCGGATGAGCTGTCTTCGCTGCTGTTACCTTCTTCCGTGGATAAAGAATTT
GATGCGGGCTATCCCTCATTCTCTCCTCTGCTGGTGGAATGGTTACAGGACAGCTGGCTGCTGGCCTCGGCTGAATTCCA
ACATGCGCAGCTGCGCAGCGGCATCTTGCTGCTGGTTTTGCTGCTGACGCCAAATCGCTATGTAGCAGGGGCTGTATCCC
GCCCGCTGGCGCAGATTAACCGTGAGCTTTTACGTCAGCAGTTTGATGAGTGGGTCAAGGATTCCGTTGAAACGGAGGTG
GCGACGCGCTCCGCTACAGCGGAGCAGGCTGCTGCGGCGACTACCCAGCTCTCTCGCTATACCCAAAACGTGACGGAATC
TGCCCGACAGGGGCAGTTGGATCCGGTGCTGTGTCGCGATCATGAAATCGATCTGATGATCGACATTCTCTCCCGTCGCC
GTAAAAACAACCCGATTGTGGTGGGGGAAGCGGGCGTCGGTAAAAGTGCGCTGATCGAAGGGCTAGCGCTGCGTATTGTG
GCGGGTGCCGTACCGGAAAGATTGCGGGATGTAGAACTGCTCACGCTCGATCTGGGAGCGATGCAGGCCGGTGCGTCAGT
TAAAGGCGAATTTGAGAAACGCTTCAAAGGTGTGATGCAGGAAGTGAAAGATGCACCACGCCCGATCATTCTGTTTATCG
ATGAAGCGCATACGCTAATTGGGGCTGGCAATCAGGCTGGCGGGCTGGATGTGTCCAACCTGCTCAAGCCTGCGCTGGCA
CGCGGTGAATTGCGCACGATCGCAGCGACGACCTGGAGTGAATACAAAAAATACGTCGAGAAAGATGCGGCGCTTTCCCG
TCGCTTCCAGCTCGTCAAGGTCGGTGAACCGAACGCGGAAGAAGCCACCGTTATCCTGCGTGGGCTGCGCAGCATCTATG
AAAAAGCACACGGCGTACTGATTGATGAAGATGCACTTCAGGCGGCGGCGCAGCTGTCGGCGCGCTATATCTCCGGTCGC
CAACTGCCCGATAAAGCCATTGACGTGCTGGATACCGCGAGTGCGCGCGTGGCGATTAACCTGACGACGCCACCGCGTGC
AGTCAGCCAGCTACAGACTCGCCTGCATCAGCAGAAAATGGAAATCACCCAGCTTGAGCGTCAGGCGCGCATCGGTCTGG
GTAATACTGAAGAGCGGTTAGTCGAACTGCGTGACGCCCGCGAAGCGGGGGCGGCATCGCTGGCACAGCTGGAAGCCGAT
TGGCAGCAGCAGAAAACACAGGTTCAACGCGTGATTGAACTGCGTACGGCGCTGTTGGATGACGAGCAAACCGTCGACTT
TGATGCGGTATCGGCAGCGGCGGAACTGGCTACCTGTGAGCAAGCACTGGAAATACTTCAGCAAGCTTCCGTGCTGGTCT
CTCCCCACGTCGATAAAATGCAGATTGCGGCAGTGATTGCCGAATGGACGGGCGTGCCGCTGAACCGAATTTCACAGGGT
GAAATGGATATCGTCACCCGCCTGCCTGAATTCCTGGGAGAGTCGATTAAAGGGCAGCAGTTAGCGATTGCCCAATTGCA
TAAACATTTACTGACTGCGCGTGCGGATCTCCGTCGTCCGGGGCGTCCGCTAGGTGCTTTCCTGCTGGTGGGGCCAAGCG
GTGTCGGTAAAACGGAAACTGTGGTTCAGATTGCCGACCTGATGTTTGGTGGACGTAACTATCTGACCACCATCAATATG
TCGGAATATCAGGAAAAACATACGGTTTCACGCCTGATCGGTTCGCCTCCGGGCTACGTCGGGTTTGGCGAAGGGGGCGT
GTTGACCGAAGCCATCCGTCAGAAGCCGTACTCCGTGGTACTGCTGGATGAAGTAGAGAAAGCGCACCCGGATGTTCTGA
ATTTGTTCTATCAGGCGTTTGATAAAGGTGAACTGGCCGATGGTGAAGGCCGTGTCATCGACTGCCGCAACGTGGTGTTC
TTCCTGACGTCCAATCTCGGGTTCCAGACGATCGTGAACTACGCCGAGCAGTCGGATGTGTTGCTGGATGCGCTCTATCC
CGAACTGGCGGCCTTCTTCAAACCTGCATTATTGGCGCGTATGGAAGTCATTCCTTATCTGCCGCTGGCGCATGACACCA
TGGTCGAGATCGTACAGGGCAAATTGTCGCGTCTGGTTTCTCTGCTGCAACAGCGCTTTGGTGCGGAAGTCATCATTGAA
GACGAGGTGCCGGAAGAGATTCTACGGTTGGCGAACCGCAGCGAAAACGGGGCACGTATGCTCGAATCAGTGATTGACGG
GGCCTTGCTGCCACCGGTTTCGCTACAGCTACTGCAACGCATGTCTGCGGGTGAACCCGTTAGCCGTATTCATTTCCGTG
TCGAAGCCGGCCAGTTCCAGACCGAGGTGGAGGGCTGA