Detailed information of component protein


Summary

Component ID T6CP008455
Component protein type TssM
T6SS ID (Type) T6SS00040 experimental (Type i1)
Strain Pectobacterium atrosepticum SCRI1043
Replicon chromosome
Sequence Protein sequence (1166 a.a.); Nucleotide sequence (3498 bp)
Reference
[1] Mattinen L, Somervuo P, Nykyri J, et al. Microarray profiling of host-extract-induced genes and characterization of the type VI secretion cluster in the potato pathogen Pectobacterium atrosepticum. Microbiology (Reading). 2008 Aug;154(Pt 8):2387-2396. doi: 10.1099/mic.0.2008/017582-0. PMID: 18667571
[2] Mattinen L, Nissinen R, Riipi T, et al. Host-extract induced changes in the secretome of the plant pathogenic bacterium Pectobacterium atrosepticum. Proteomics. 2007 Oct;7(19):3527-37. PMID: 17726675
[3] Liu H, Coulthurst SJ, Pritchard L, et al. Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum. PLoS Pathog. 2008 Jun 20;4(6):e1000093. doi: 10.1371/journal.ppat.1000093. PMID: 18566662
[4] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[5] Bernard CS, Brunet YR, Gavioli M, et al. Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. J Bacteriol. 2011 May;193(9):2158-67. doi: 10.1128/JB.00029-11. Epub 2011 Mar 4. PMID: 21378190
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ECA_RS16925
Coordinate (Strand) 3845233..3848730 (-)
NCBI ID WP_011094940.1
RefSeq NC_004547
Uniprot ID Q6D1L4_PECAS
KEGG ID eca:ECA3432
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 7.1e-77 506..819
IcmF-related_N PF14331.8 2.1e-67 202..452
IcmF_C PF06744.14 8.2e-23 1038..1144
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-19 MFKTIITFLRKQLPKLKPS
Transmembrane helix 20-39 WLLLGALLWVVVLVLAWWLG
Outside 40-53 PRLTVGESRPLQGI
Transmembrane helix 54-76 WGRVVFTLVWLWLAFSYSAWLVW
Inside 77-449 RRVQQMRAERHEHQVIEQDPLQVYVDGQQTFLDRWLEAFQTQLGKKALYAMPWYLTIGLAGSGKSSLIHRANPANKMNPKLDAALRDVAAGQLVSSWVGESAVIWDPSGQLLAQPELAGDSLGLRHARLWQHLLQWLSENRRRQPLNGLVLTLDISWLAQAGVADRKAYAQVMRARLQEISVNINTRLPVYIALTRLDMLSGFDKVYRQLNRDARQAILGVTFTPQASNSKGWLEELERFWDEWVTHLNDNLPDMLLTQSDRSVRNSLFSFVRQLAGVKDYVMEVLTETLATGEDRAFLIRGVYVSSVYQQGVPFDAFAQSASRRYQLPEPINAALRGESNTFFVQRLFPDVIFPEASLAGENRLHSLYRRRR
Transmembrane helix 450-472 LSIGVGCMLLASLALVGSWHHFY
Outside 473-1165 RVNEEAGRNVLTKAQAFIGTNELEGQPGYGYQQLPRLNLIRDATLSFGNYHERTPLLADLGLYQGGKIGPYVEGTYLQMLNQRFLPAVMQGLLEDLNQAPANSEQKLTILRVMRMLDDASGRNKVLVEQFMAQRWQKAFPAQGNVQEQLMQHLDYALEHTDWHKAREQKYAAAISTFTPFNQPITLAQQELSKLPMYQRVYQSLVMKATQVLPPDLAIRDEVGPTFDPVFSLRNDKAGSVPRLLTYPGFSDYYLKQDKALLELTALDAWVLGQRERAQFSEADRREILRQVNDRYITDYINQWQKVLANIDVQTLDTPEQALDILTDITGNDQPFQRVLTTVSDNTRIRKLSDDDNDTAQGINTRTGRPFMTINAALSGRGEQGPLVQEVNQKLTDLYHYLDQIVNATDPGQAALKAVQARQGNKFADPVFALQQYARSLPAPLDRWVGQLAGESASLVTGLAMSSLNQEWLDKVVTPFNEKLADRYPFDPSSNKDVPLSEMEMFFMTGGTLDSFYQTNLKAMMESGMLEEGMASPLQAELVKQLERATRIRQTLFNAQGSLEVHFVLEPLELTANKRRSVLNLDGQLLEYSHGRRQKTPLVWPNSMRDGAESKLTLVPDDRERSPRSLSFSGPWAMFRLVNSDQLTQVNENTFDVRFSLENGAMTYRVYTDASHNPFAGGLFSQFTLPDSLY


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9945 - -


  Protein sequence: 1166 a.a.    

>T6CP008455 NC_004547:c3848730-3845233 [Pectobacterium atrosepticum SCRI1043] [TssM]
MFKTIITFLRKQLPKLKPSWLLLGALLWVVVLVLAWWLGPRLTVGESRPLQGIWGRVVFTLVWLWLAFSYSAWLVWRRVQ
QMRAERHEHQVIEQDPLQVYVDGQQTFLDRWLEAFQTQLGKKALYAMPWYLTIGLAGSGKSSLIHRANPANKMNPKLDAA
LRDVAAGQLVSSWVGESAVIWDPSGQLLAQPELAGDSLGLRHARLWQHLLQWLSENRRRQPLNGLVLTLDISWLAQAGVA
DRKAYAQVMRARLQEISVNINTRLPVYIALTRLDMLSGFDKVYRQLNRDARQAILGVTFTPQASNSKGWLEELERFWDEW
VTHLNDNLPDMLLTQSDRSVRNSLFSFVRQLAGVKDYVMEVLTETLATGEDRAFLIRGVYVSSVYQQGVPFDAFAQSASR
RYQLPEPINAALRGESNTFFVQRLFPDVIFPEASLAGENRLHSLYRRRRLSIGVGCMLLASLALVGSWHHFYRVNEEAGR
NVLTKAQAFIGTNELEGQPGYGYQQLPRLNLIRDATLSFGNYHERTPLLADLGLYQGGKIGPYVEGTYLQMLNQRFLPAV
MQGLLEDLNQAPANSEQKLTILRVMRMLDDASGRNKVLVEQFMAQRWQKAFPAQGNVQEQLMQHLDYALEHTDWHKAREQ
KYAAAISTFTPFNQPITLAQQELSKLPMYQRVYQSLVMKATQVLPPDLAIRDEVGPTFDPVFSLRNDKAGSVPRLLTYPG
FSDYYLKQDKALLELTALDAWVLGQRERAQFSEADRREILRQVNDRYITDYINQWQKVLANIDVQTLDTPEQALDILTDI
TGNDQPFQRVLTTVSDNTRIRKLSDDDNDTAQGINTRTGRPFMTINAALSGRGEQGPLVQEVNQKLTDLYHYLDQIVNAT
DPGQAALKAVQARQGNKFADPVFALQQYARSLPAPLDRWVGQLAGESASLVTGLAMSSLNQEWLDKVVTPFNEKLADRYP
FDPSSNKDVPLSEMEMFFMTGGTLDSFYQTNLKAMMESGMLEEGMASPLQAELVKQLERATRIRQTLFNAQGSLEVHFVL
EPLELTANKRRSVLNLDGQLLEYSHGRRQKTPLVWPNSMRDGAESKLTLVPDDRERSPRSLSFSGPWAMFRLVNSDQLTQ
VNENTFDVRFSLENGAMTYRVYTDASHNPFAGGLFSQFTLPDSLY*

  Nucleotide sequence: 3498 bp    

>T6CP008455 NC_004547:c3848730-3845233 [Pectobacterium atrosepticum SCRI1043] [TssM]
ATGTTTAAAACAATCATAACCTTTCTACGCAAGCAGTTGCCTAAACTGAAACCCTCCTGGCTGCTTTTGGGAGCGCTACT
GTGGGTCGTAGTGTTGGTTCTAGCCTGGTGGCTTGGGCCGCGTTTGACGGTGGGCGAGTCACGCCCGTTGCAGGGAATCT
GGGGCCGCGTGGTGTTCACGCTGGTCTGGCTGTGGTTGGCGTTCTCCTACAGCGCCTGGCTGGTCTGGCGTCGCGTACAG
CAGATGCGCGCAGAGCGCCATGAACATCAGGTGATTGAGCAGGATCCCTTACAGGTGTATGTCGACGGTCAGCAGACGTT
TCTCGATCGCTGGCTGGAGGCATTTCAGACCCAGTTGGGTAAAAAAGCGTTGTATGCGATGCCCTGGTATTTGACGATTG
GTCTGGCCGGTAGTGGCAAAAGCAGCCTGATCCATCGCGCCAATCCGGCAAACAAAATGAATCCGAAGTTGGATGCCGCG
CTGCGAGACGTAGCGGCAGGCCAGCTGGTGAGTAGCTGGGTCGGGGAATCCGCTGTCATCTGGGATCCTAGTGGACAATT
ACTGGCGCAGCCTGAGCTGGCGGGTGATTCGCTCGGGCTGCGCCATGCCCGACTGTGGCAGCACCTGCTACAATGGCTCA
GTGAAAATCGTCGCCGCCAGCCGCTCAATGGTCTGGTTCTCACGCTGGATATTTCCTGGTTGGCACAGGCCGGTGTCGCG
GATCGTAAAGCCTATGCGCAAGTCATGCGCGCGCGTTTGCAGGAAATTTCTGTCAACATCAATACGCGCTTACCGGTGTA
TATCGCGCTGACTCGACTGGATATGCTGAGCGGCTTCGATAAGGTCTATCGCCAGTTAAACCGCGATGCGCGTCAGGCCA
TTTTAGGCGTGACGTTTACTCCGCAGGCGAGCAACAGCAAAGGTTGGCTGGAAGAACTAGAGCGTTTCTGGGACGAGTGG
GTCACGCACCTGAATGACAACCTGCCAGACATGCTGCTGACGCAATCGGACAGAAGTGTGCGCAATTCGCTGTTCTCGTT
CGTCCGCCAGTTGGCTGGAGTGAAAGATTACGTGATGGAAGTGCTGACGGAAACGTTAGCGACGGGCGAGGATCGCGCGT
TCCTGATCCGTGGCGTCTACGTCAGTTCTGTCTATCAACAGGGGGTGCCGTTCGATGCTTTTGCGCAGTCGGCTTCCCGA
CGCTATCAACTGCCAGAGCCGATTAACGCTGCGCTGCGCGGTGAGTCGAACACGTTCTTTGTGCAACGTCTTTTCCCTGA
TGTCATTTTTCCGGAGGCCAGTCTGGCGGGGGAAAACCGACTGCATAGTCTCTATCGTCGTCGTCGGCTCAGTATCGGCG
TGGGCTGTATGCTGCTCGCCAGTCTGGCGCTAGTCGGCAGTTGGCACCATTTCTATCGGGTCAATGAAGAAGCCGGACGT
AATGTACTGACGAAAGCGCAGGCGTTTATCGGCACCAATGAGCTGGAAGGGCAGCCGGGCTATGGCTATCAGCAGTTGCC
GCGTCTGAATTTGATTCGTGATGCGACCTTATCCTTTGGTAATTACCACGAACGTACGCCGCTGCTAGCCGATCTTGGCC
TGTATCAGGGCGGTAAGATTGGGCCTTATGTTGAAGGCACTTATCTGCAAATGCTGAACCAGCGCTTTCTCCCTGCGGTG
ATGCAGGGGCTGTTGGAAGATTTGAATCAGGCACCCGCTAATAGCGAGCAAAAGCTGACGATTCTGCGTGTGATGCGGAT
GTTGGATGATGCGTCAGGACGCAATAAAGTGCTGGTCGAACAGTTTATGGCGCAGCGCTGGCAGAAGGCGTTTCCCGCAC
AGGGCAATGTGCAGGAACAACTGATGCAGCATCTGGATTACGCCCTTGAGCATACCGACTGGCATAAGGCCCGTGAGCAA
AAATATGCGGCGGCGATCAGTACCTTTACGCCATTTAATCAGCCGATTACGTTGGCTCAACAGGAGCTGAGCAAGCTGCC
GATGTATCAGCGCGTTTATCAGAGTTTGGTGATGAAAGCGACGCAGGTGCTGCCGCCGGATTTAGCGATCCGTGATGAAG
TCGGCCCGACGTTTGATCCGGTGTTTTCCTTGCGTAATGACAAAGCGGGAAGTGTGCCGCGGCTGCTGACGTATCCCGGT
TTCAGTGATTATTACCTCAAGCAGGACAAAGCGCTGTTAGAACTGACGGCATTGGACGCCTGGGTTCTGGGCCAGCGTGA
GCGTGCGCAGTTTAGCGAGGCAGACCGTCGGGAAATTCTGCGTCAGGTGAACGATCGCTACATTACGGATTACATCAACC
AGTGGCAGAAAGTGTTGGCGAACATTGATGTGCAGACGCTCGATACGCCGGAGCAGGCGCTCGATATTCTCACGGATATT
ACCGGCAACGATCAGCCCTTCCAGCGAGTGTTGACCACGGTCAGTGATAACACCCGAATCCGTAAGCTGTCCGACGATGA
TAACGACACGGCGCAAGGTATCAACACGCGCACTGGTCGTCCGTTTATGACTATCAATGCGGCACTGAGCGGACGTGGTG
AGCAGGGGCCATTGGTGCAAGAGGTTAATCAGAAGCTGACCGACCTCTATCACTACCTCGATCAGATCGTCAACGCGACC
GATCCAGGGCAGGCAGCGTTGAAAGCGGTGCAGGCGCGGCAGGGAAATAAATTTGCCGATCCAGTCTTCGCCTTACAGCA
ATACGCCCGTAGCCTTCCCGCGCCACTGGATCGCTGGGTAGGACAATTAGCCGGAGAAAGCGCCAGTCTGGTGACTGGGC
TTGCGATGTCGTCGCTGAATCAAGAGTGGCTGGATAAAGTTGTGACGCCATTCAATGAGAAGCTGGCGGATCGTTACCCC
TTCGACCCGTCATCGAACAAGGATGTGCCGCTATCGGAAATGGAAATGTTCTTCATGACTGGCGGGACGCTGGACAGCTT
CTATCAAACCAACCTCAAGGCGATGATGGAAAGCGGCATGCTGGAAGAAGGGATGGCGTCGCCTTTACAGGCTGAGCTGG
TGAAACAGCTGGAGCGTGCTACCCGCATCCGCCAGACCCTGTTTAATGCGCAGGGTAGTCTGGAAGTGCACTTTGTTCTG
GAGCCGCTGGAACTGACGGCGAACAAGCGCCGCAGCGTACTCAATCTGGACGGACAACTGCTGGAATACAGTCACGGTCG
TCGTCAGAAAACGCCGCTGGTGTGGCCAAACAGTATGCGAGATGGGGCTGAGAGCAAGCTAACTCTGGTGCCGGACGATC
GTGAGCGCTCACCACGTAGCCTGAGCTTTAGCGGGCCGTGGGCGATGTTCCGGTTAGTCAACAGCGACCAGCTAACGCAG
GTTAATGAGAACACCTTCGACGTGCGCTTCTCGCTGGAAAACGGCGCGATGACGTATCGCGTATATACCGATGCCAGCCA
TAACCCGTTTGCTGGTGGTCTGTTCAGCCAATTCACGCTGCCTGACTCGCTTTATTAA