Detailed information of component protein


Summary

Component ID T6CP008452
Component protein type TssI
T6SS ID (Type) T6SS00040 experimental (Type i1)
Strain Pectobacterium atrosepticum SCRI1043
Replicon chromosome
Sequence Protein sequence (679 a.a.); Nucleotide sequence (2037 bp)
Reference
[1] Mattinen L, Somervuo P, Nykyri J, et al. Microarray profiling of host-extract-induced genes and characterization of the type VI secretion cluster in the potato pathogen Pectobacterium atrosepticum. Microbiology (Reading). 2008 Aug;154(Pt 8):2387-2396. doi: 10.1099/mic.0.2008/017582-0. PMID: 18667571
[2] Mattinen L, Nissinen R, Riipi T, et al. Host-extract induced changes in the secretome of the plant pathogenic bacterium Pectobacterium atrosepticum. Proteomics. 2007 Oct;7(19):3527-37. PMID: 17726675
[3] Liu H, Coulthurst SJ, Pritchard L, et al. Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum. PLoS Pathog. 2008 Jun 20;4(6):e1000093. doi: 10.1371/journal.ppat.1000093. PMID: 18566662
[4] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[5] Bernard CS, Brunet YR, Gavioli M, et al. Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. J Bacteriol. 2011 May;193(9):2158-67. doi: 10.1128/JB.00029-11. Epub 2011 Mar 4. PMID: 21378190
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ECA_RS16900
Coordinate (Strand) 3839060..3841096 (-)
NCBI ID WP_011094936.1
RefSeq NC_004547
Uniprot ID Q6D1L8_PECAS
KEGG ID eca:ECA3427
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 9.3e-90 30..327
Phage_base_V PF04717.14 9.1e-13 384..450
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-678 MANSTGLQFTVKVGALEAGTFAVVDFSLDEGLNRPFSLSLSLASALPDVDFGAVLDQPCELMIWYEGELKRRVSGIISGFTQGDTGFRRTRYQAEVRPALWRLGLRTNARIFQAQKPEAIIGTLLEEAGITDYAFALRHEHAVREYCVQYRESDLAFITRLAAEEGLYFFHEFEEGKHRVVFADDAGALTKGPELFFNLATQGLSEGEYVRRFHYAERVSTAEVELKDYNFKTPAYGLSHKKMSGELEHQRESYQHYDYPGRYKQDPSGKAFSGYRLDALRSGAVTSEGESNCAGLMPGNTFTLTEHPNATLNAVWQTVSVTHVGQQPQALEEESGGEPTTMSNSFAVVKGTTTWRAAMPYKPMVDGPQIATVVGPAGEEIYCDQFGRVKLQFPWDRYGASNDQSSCWVRVSQGWAGGQYGMIAIPRISHEVIVSFLEGDPDQPIITGRTFHATNPTPYVLPAHKTRTTLRTDTHKGSGFNELRFEDEATREQIYYHAQKDMDGVINNIHRQSVGRDQHLSVAQDQFQRVDRHRHRTIGKDDFENIGQDHHQEIGRSFIQKIGSSFKRFIGGGEITRIEGSRQTTMSGSEETLIGAHQRILVNTDSYLKAADIVLEAGQALTIKAPGGFIKIDSAGVTISGTIVKINDGGAAGVGTAPVSITPEDPSKPTLPDAPDRR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9644 - -


  Protein sequence: 679 a.a.    

>T6CP008452 NC_004547:c3841096-3839060 [Pectobacterium atrosepticum SCRI1043] [TssI]
MANSTGLQFTVKVGALEAGTFAVVDFSLDEGLNRPFSLSLSLASALPDVDFGAVLDQPCELMIWYEGELKRRVSGIISGF
TQGDTGFRRTRYQAEVRPALWRLGLRTNARIFQAQKPEAIIGTLLEEAGITDYAFALRHEHAVREYCVQYRESDLAFITR
LAAEEGLYFFHEFEEGKHRVVFADDAGALTKGPELFFNLATQGLSEGEYVRRFHYAERVSTAEVELKDYNFKTPAYGLSH
KKMSGELEHQRESYQHYDYPGRYKQDPSGKAFSGYRLDALRSGAVTSEGESNCAGLMPGNTFTLTEHPNATLNAVWQTVS
VTHVGQQPQALEEESGGEPTTMSNSFAVVKGTTTWRAAMPYKPMVDGPQIATVVGPAGEEIYCDQFGRVKLQFPWDRYGA
SNDQSSCWVRVSQGWAGGQYGMIAIPRISHEVIVSFLEGDPDQPIITGRTFHATNPTPYVLPAHKTRTTLRTDTHKGSGF
NELRFEDEATREQIYYHAQKDMDGVINNIHRQSVGRDQHLSVAQDQFQRVDRHRHRTIGKDDFENIGQDHHQEIGRSFIQ
KIGSSFKRFIGGGEITRIEGSRQTTMSGSEETLIGAHQRILVNTDSYLKAADIVLEAGQALTIKAPGGFIKIDSAGVTIS
GTIVKINDGGAAGVGTAPVSITPEDPSKPTLPDAPDRR*

  Nucleotide sequence: 2037 bp    

>T6CP008452 NC_004547:c3841096-3839060 [Pectobacterium atrosepticum SCRI1043] [TssI]
ATGGCAAACAGTACAGGATTACAGTTTACCGTAAAGGTCGGTGCTCTGGAGGCCGGCACCTTTGCGGTGGTGGATTTCAG
TCTGGATGAGGGGTTGAATCGGCCCTTCAGCCTGTCGCTGAGTCTGGCGAGCGCGTTGCCGGATGTCGATTTCGGTGCGG
TGCTGGATCAGCCGTGCGAACTGATGATTTGGTATGAAGGCGAACTGAAGCGTCGGGTCAGCGGGATTATCAGTGGGTTT
ACTCAAGGCGACACCGGATTTCGGCGCACGCGCTATCAGGCGGAAGTCCGTCCGGCGCTGTGGCGACTGGGTCTTCGCAC
CAACGCCCGCATCTTTCAGGCGCAGAAGCCGGAAGCGATTATCGGCACATTGCTGGAAGAAGCAGGCATTACCGACTACG
CGTTTGCGCTGCGCCATGAGCACGCCGTGCGGGAATACTGCGTACAGTATCGGGAAAGTGATTTAGCCTTTATCACTCGG
CTGGCCGCAGAGGAAGGCCTGTACTTCTTCCACGAGTTTGAGGAGGGCAAGCATCGAGTGGTGTTTGCCGATGATGCGGG
TGCGCTGACCAAAGGGCCGGAGCTGTTCTTCAATCTCGCCACGCAGGGGTTAAGTGAGGGCGAATATGTCCGGCGTTTCC
ACTACGCGGAGCGGGTGAGCACGGCCGAAGTGGAACTGAAGGACTACAACTTCAAGACGCCGGCTTACGGACTGTCACAC
AAGAAGATGAGCGGTGAGCTGGAGCACCAGCGTGAAAGCTATCAGCATTACGACTATCCGGGACGTTATAAGCAAGATCC
GAGCGGCAAGGCGTTCAGCGGCTATCGTCTGGATGCGCTGCGCTCAGGGGCGGTGACAAGCGAAGGGGAGTCCAACTGCG
CGGGGCTGATGCCGGGCAATACCTTTACCCTGACGGAGCATCCGAATGCGACGCTGAACGCAGTGTGGCAGACAGTGAGT
GTGACGCACGTCGGGCAGCAGCCGCAGGCGCTGGAAGAGGAAAGCGGCGGCGAACCGACGACCATGAGCAACAGCTTTGC
GGTGGTGAAGGGCACAACGACGTGGCGCGCGGCGATGCCGTACAAACCGATGGTGGACGGCCCGCAAATCGCGACGGTGG
TGGGTCCGGCGGGTGAAGAAATTTACTGTGATCAGTTCGGCCGGGTAAAACTGCAATTCCCGTGGGATCGCTATGGCGCG
AGCAACGACCAGAGCTCTTGCTGGGTGCGCGTCAGTCAGGGCTGGGCGGGTGGCCAGTACGGCATGATCGCTATCCCACG
TATCAGCCATGAAGTGATCGTCAGCTTCCTCGAAGGTGACCCAGACCAGCCTATTATCACTGGGCGTACGTTCCACGCGA
CCAACCCGACGCCGTACGTTTTACCCGCACATAAAACGCGCACGACGCTGCGTACTGATACGCACAAAGGCAGTGGCTTC
AACGAACTGCGTTTCGAGGATGAGGCGACGCGTGAACAAATTTACTATCACGCTCAGAAAGATATGGATGGTGTCATCAA
TAACATCCATCGGCAGAGTGTGGGGCGCGATCAGCATCTCAGCGTCGCTCAGGATCAGTTCCAACGAGTCGATCGTCACC
GTCACCGAACGATAGGCAAAGATGACTTTGAAAATATCGGTCAGGATCATCATCAGGAAATTGGCCGGAGTTTTATTCAG
AAAATAGGCTCTTCGTTCAAGCGCTTTATCGGTGGGGGAGAGATTACCCGTATCGAAGGGAGCCGCCAGACCACGATGTC
CGGTTCTGAAGAGACGCTGATCGGGGCGCATCAGCGTATTCTGGTCAATACGGACAGCTACCTGAAAGCCGCAGATATCG
TGCTGGAAGCGGGGCAGGCTCTGACTATTAAAGCGCCTGGCGGTTTTATCAAGATTGATAGCGCGGGTGTCACAATTTCC
GGGACGATTGTGAAAATCAACGATGGTGGTGCGGCTGGCGTAGGGACTGCGCCGGTGTCGATTACGCCAGAAGACCCCTC
GAAGCCTACGCTGCCGGATGCGCCAGACAGACGTTAA