Detailed information of component protein


Summary

Component ID T6CP008398
Component protein type TssA
T6SS ID (Type) T6SS00042 experimental (Type i1)
Strain Pseudomonas syringae pv. tomato str. DC3000
Replicon chromosome
Sequence Protein sequence (519 a.a.); Nucleotide sequence (1557 bp)
Reference
[1] Haapalainen M, Mosorin H, Dorati F, et al. Hcp2, a secreted protein of the phytopathogen Pseudomonas syringae pv. tomato DC3000, is required for fitness for competition against bacteria and yeasts. J Bacteriol. 2012 Sep;194(18):4810-22. doi: 10.1128/JB.00611-12. Epub 2012 Jun 29. PMID: 22753062
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSPTO_RS28125
Coordinate (Strand) 6178764..6180320 (-)
NCBI ID WP_011105494.1
RefSeq NC_004578
Uniprot ID Q87U74_PSESM
KEGG ID pst:PSPTO_5434
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasJ PF16989.7 2.4e-82 225..483
ImpA_N PF06812.14 1.5e-29 17..127
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-518 MSYSSKLSAHYLELAKAPVSPASFAGDDVRFSVEFESLESELGKAQSMHEAGQIDWLKIRENSESLLRTQSKDLRVGVWLAWALYQRESFQGLLAGLGVLHHLCEQHWADIHPKKARTRAAAIGWLVPRLEQVLNENVALKEQLPMFRRLVEHLEGLDKACTEHLGDDAPLLLPISRRLKSMVQRAADNQPEPGVVGAAVAQVKQAATQLFTPGAPIDNEKEAHKALRAQQENARPLCAWWLKQKASDLRALRLNRTLLWLPIDAVPERNAEQITALRGLPADKLKAYRDRYEQAKYADLLVELEASLAKAPFWFDGQRMVWECLQGLNAEMAMREVEIHFALLIQRLPGIIELRYHDGTPFADPATRAWISASVLPHLQTNNAPRKVEVTDTQPAWEVALEAALLVLRKEGLKAAVQFLKQGLQKAQGGRARFFWQFALARLCFVAKKYELAKAQLETLDQTLQESGLHAWEPDLALEVLHLLHSCCELLPQNQVVRERKEEIYRRLCHLDLEVVLE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9006 - -


  Protein sequence: 519 a.a.    

>T6CP008398 NC_004578:c6180320-6178764 [Pseudomonas syringae pv. tomato str. DC3000] [TssA]
MSYSSKLSAHYLELAKAPVSPASFAGDDVRFSVEFESLESELGKAQSMHEAGQIDWLKIRENSESLLRTQSKDLRVGVWL
AWALYQRESFQGLLAGLGVLHHLCEQHWADIHPKKARTRAAAIGWLVPRLEQVLNENVALKEQLPMFRRLVEHLEGLDKA
CTEHLGDDAPLLLPISRRLKSMVQRAADNQPEPGVVGAAVAQVKQAATQLFTPGAPIDNEKEAHKALRAQQENARPLCAW
WLKQKASDLRALRLNRTLLWLPIDAVPERNAEQITALRGLPADKLKAYRDRYEQAKYADLLVELEASLAKAPFWFDGQRM
VWECLQGLNAEMAMREVEIHFALLIQRLPGIIELRYHDGTPFADPATRAWISASVLPHLQTNNAPRKVEVTDTQPAWEVA
LEAALLVLRKEGLKAAVQFLKQGLQKAQGGRARFFWQFALARLCFVAKKYELAKAQLETLDQTLQESGLHAWEPDLALEV
LHLLHSCCELLPQNQVVRERKEEIYRRLCHLDLEVVLE*

  Nucleotide sequence: 1557 bp    

>T6CP008398 NC_004578:c6180320-6178764 [Pseudomonas syringae pv. tomato str. DC3000] [TssA]
ATGTCCTACTCAAGCAAACTTTCTGCTCACTACCTGGAACTCGCCAAGGCCCCTGTTTCGCCTGCTAGTTTTGCGGGCGA
TGACGTTCGTTTTTCGGTCGAGTTCGAGTCGCTGGAAAGCGAGTTGGGTAAAGCCCAGTCCATGCATGAAGCAGGGCAGA
TCGACTGGCTTAAAATCCGTGAAAACAGTGAGAGCCTGCTTCGCACCCAGTCCAAGGACTTGCGGGTAGGTGTCTGGCTT
GCATGGGCGCTTTATCAGCGAGAGTCCTTCCAGGGCCTGTTGGCCGGTCTCGGTGTTTTGCACCATCTTTGCGAGCAGCA
CTGGGCTGACATTCACCCGAAAAAGGCCCGCACGCGCGCCGCTGCCATTGGCTGGCTGGTGCCGCGTCTCGAGCAGGTGC
TGAACGAAAACGTCGCGCTCAAGGAGCAGTTGCCAATGTTCCGGCGTCTGGTCGAACACCTTGAAGGCCTCGATAAAGCG
TGCACCGAACATTTGGGCGACGACGCACCTTTGCTACTGCCGATCTCCCGCCGTCTGAAAAGCATGGTCCAGCGCGCTGC
TGACAACCAGCCCGAGCCTGGTGTAGTCGGCGCAGCGGTTGCGCAGGTCAAGCAGGCTGCCACACAACTCTTCACGCCTG
GTGCGCCTATCGATAACGAAAAGGAAGCGCACAAGGCCCTGCGCGCCCAGCAGGAAAACGCGCGCCCTTTATGTGCATGG
TGGCTTAAACAGAAAGCCAGCGATCTGCGAGCCTTGCGGCTTAATCGCACACTGCTGTGGCTGCCGATCGATGCTGTTCC
GGAGCGTAACGCCGAGCAAATCACTGCCCTGCGAGGCCTGCCGGCAGACAAACTCAAGGCTTATCGGGATCGCTACGAGC
AGGCCAAATATGCCGATCTCTTGGTCGAGCTGGAAGCGAGCCTGGCGAAGGCGCCTTTCTGGTTCGACGGCCAGCGAATG
GTATGGGAATGCCTGCAAGGCCTGAACGCAGAGATGGCGATGCGTGAAGTGGAAATCCACTTCGCGCTTTTGATTCAGCG
CCTGCCCGGCATCATCGAATTGCGCTATCACGATGGAACGCCGTTCGCCGACCCGGCGACGCGTGCCTGGATCAGCGCAA
GCGTCTTGCCGCATCTGCAAACCAACAACGCACCGCGCAAGGTGGAAGTTACCGATACCCAGCCAGCCTGGGAAGTGGCC
CTTGAAGCGGCGTTGCTCGTCCTGCGCAAAGAGGGCTTGAAAGCCGCTGTGCAGTTCCTCAAGCAAGGGCTGCAAAAGGC
GCAGGGCGGACGCGCTCGATTTTTCTGGCAGTTCGCTCTCGCCCGACTGTGCTTCGTGGCCAAAAAATACGAACTGGCCA
AGGCGCAACTCGAGACGCTGGATCAGACATTACAGGAGTCGGGCCTGCATGCATGGGAGCCCGATCTTGCGCTGGAAGTG
CTGCATCTGCTGCATAGCTGCTGTGAGCTGCTACCACAGAACCAAGTGGTGCGCGAACGCAAGGAAGAGATTTATCGCAG
GCTGTGCCACCTAGACCTCGAAGTGGTACTCGAATAG