Detailed information of component protein


Summary

Component ID T6CP005739
Component protein type TssI
T6SS ID (Type) T6SS00540 experimental (Type i3)
Strain Pseudomonas fluorescens F113
Replicon chromosome
Sequence Protein sequence (646 a.a.); Nucleotide sequence (1938 bp)
Reference
[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[2] Redondo-Nieto M, Barret M, Morrissey J, et al. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics. 2013 Jan 25;14:54. doi: 10.1186/1471-2164-14-54. PMID: 23350846
[3] Durán D, Bernal P, Vazquez-Arias D, et al. Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome. Sci Rep. 2021 Mar 11;11(1):5772. doi: 10.1038/s41598-021-85218-1. PMID: 33707614
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSF113_RS59865
Coordinate (Strand) 6692072..6694009 (+)
NCBI ID WP_014340892.1
RefSeq NC_016830
Uniprot ID G8Q725_PSEF3
KEGG ID pfe:PSF113_5807
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 9.9e-107 30..329
Phage_base_V PF04717.14 1.7e-14 386..453
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-645 MLFNQATRLAKITSPLGPEVLLLKDMGGGEELGRLFNYELQLHSLDNAIDLNQVLGKPMCVSLQLDGGGERYFHGIVARFSQNVDQGQFASYQATLRPWFWLLTRTSDCRIFQNLTIPQIIKQVFRDLGFSDFEDALSRPYREWEYCVQYRETSFDFVSRLMEQEGIYYFFRHEQGRHVLVLADAYGAHTTAPGYGSVPYYPKNEQQRERDHIHDWHLAQEVQPGSLELNDYDFQRPSARIDVRSAMPRPHTAGDYPLYDYPGTYVQSQDGEHYARTRIEALQTQHEQVELAGNARGLGSGHLFSLTGFTRQDQNREYLIVGARYYLSQESGETGGGAPTAQFESSLTCIDAQQSYRPLPITHRPIVKGPQTALVVGPKGEEIWTDQFGRVKVHFYWDRHDQSNENSSCWIRVSQAWAGKNWGSMQIPRIGQEVIVSFLEGDPDRPIITGRVYNAEQTVPYDLPANATQSGMKSRSSKGGTPANFNEIRMEDKKGAEQLYIHAERNQDIVVEVDESHSVGHDRNKSIGHNETVTIGNNRLRIVKQEDILSVGQRKTDSISQSYVIEVGENLRLVCGESILELNASGQINLTGVQISFYASGDAEFNTGGVLHLNNGGGPGATPDGQGVKGSIDANIQAAFPAPKG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9937 - -


  Protein sequence: 646 a.a.    

>T6CP005739 NC_016830:6692072-6694009 [Pseudomonas fluorescens F113] [TssI]
MLFNQATRLAKITSPLGPEVLLLKDMGGGEELGRLFNYELQLHSLDNAIDLNQVLGKPMCVSLQLDGGGERYFHGIVARF
SQNVDQGQFASYQATLRPWFWLLTRTSDCRIFQNLTIPQIIKQVFRDLGFSDFEDALSRPYREWEYCVQYRETSFDFVSR
LMEQEGIYYFFRHEQGRHVLVLADAYGAHTTAPGYGSVPYYPKNEQQRERDHIHDWHLAQEVQPGSLELNDYDFQRPSAR
IDVRSAMPRPHTAGDYPLYDYPGTYVQSQDGEHYARTRIEALQTQHEQVELAGNARGLGSGHLFSLTGFTRQDQNREYLI
VGARYYLSQESGETGGGAPTAQFESSLTCIDAQQSYRPLPITHRPIVKGPQTALVVGPKGEEIWTDQFGRVKVHFYWDRH
DQSNENSSCWIRVSQAWAGKNWGSMQIPRIGQEVIVSFLEGDPDRPIITGRVYNAEQTVPYDLPANATQSGMKSRSSKGG
TPANFNEIRMEDKKGAEQLYIHAERNQDIVVEVDESHSVGHDRNKSIGHNETVTIGNNRLRIVKQEDILSVGQRKTDSIS
QSYVIEVGENLRLVCGESILELNASGQINLTGVQISFYASGDAEFNTGGVLHLNNGGGPGATPDGQGVKGSIDANIQAAF
PAPKG*

  Nucleotide sequence: 1938 bp    

>T6CP005739 NC_016830:6692072-6694009 [Pseudomonas fluorescens F113] [TssI]
ATGTTATTCAACCAAGCCACCCGCCTGGCCAAGATCACCAGCCCCCTGGGACCTGAGGTACTGCTGCTCAAGGACATGGG
CGGCGGCGAAGAACTGGGGCGGCTGTTCAACTATGAGTTGCAGCTGCACTCGCTGGATAACGCCATCGACCTCAATCAGG
TGCTCGGCAAGCCCATGTGCGTGAGCCTGCAACTCGATGGCGGTGGCGAGCGTTATTTCCATGGCATCGTGGCCCGTTTC
AGCCAGAACGTCGACCAGGGCCAGTTCGCCAGTTACCAGGCCACCCTGCGCCCGTGGTTTTGGCTGCTGACCCGTACCTC
CGATTGCCGGATTTTCCAGAACCTCACCATCCCGCAGATCATCAAGCAGGTGTTCCGCGACTTGGGCTTTTCCGATTTCG
AAGACGCCCTGAGCCGGCCTTATCGCGAGTGGGAATACTGCGTGCAGTATCGCGAGACCAGTTTCGACTTCGTCAGCCGC
TTGATGGAGCAGGAAGGGATCTACTACTTCTTCCGCCATGAGCAAGGTCGACATGTGCTGGTGCTGGCCGACGCTTATGG
AGCCCACACCACGGCGCCCGGCTACGGCTCGGTGCCCTACTACCCCAAGAACGAGCAGCAGCGCGAGCGTGACCACATAC
ACGACTGGCACCTGGCCCAGGAAGTCCAGCCCGGCTCGCTGGAGCTCAACGACTACGACTTCCAACGCCCCAGCGCACGC
ATCGACGTACGCTCGGCCATGCCCCGCCCACACACTGCCGGCGACTATCCCCTGTACGACTACCCTGGCACCTACGTGCA
GAGCCAGGACGGCGAACACTACGCCCGCACCCGCATCGAAGCCTTGCAGACCCAGCACGAACAGGTCGAGCTGGCCGGTA
ACGCTCGGGGCCTGGGCTCGGGGCATCTGTTCAGCCTCACCGGCTTCACCCGCCAGGACCAGAACCGCGAATACCTGATT
GTCGGCGCCCGCTACTACCTCTCCCAGGAAAGCGGCGAAACCGGCGGCGGCGCGCCGACAGCACAGTTCGAAAGCAGCCT
GACCTGCATCGACGCCCAGCAAAGCTATCGGCCGCTGCCCATCACCCATCGCCCTATCGTCAAGGGCCCGCAGACTGCCT
TAGTGGTGGGTCCCAAGGGCGAGGAAATCTGGACCGATCAGTTCGGCCGAGTGAAGGTGCACTTCTATTGGGATCGGCAC
GACCAGTCCAACGAAAACAGCTCGTGCTGGATTCGCGTGTCGCAGGCATGGGCCGGGAAAAACTGGGGCTCGATGCAGAT
CCCGCGCATTGGCCAGGAAGTGATCGTCAGTTTCCTCGAAGGCGACCCGGATCGGCCGATCATCACCGGTCGCGTCTACA
ATGCCGAACAGACCGTGCCCTATGACTTGCCCGCCAATGCCACCCAGAGCGGGATGAAAAGCCGTTCGAGCAAGGGCGGC
ACGCCGGCGAATTTCAACGAAATCCGCATGGAAGACAAGAAAGGCGCCGAGCAACTGTACATCCACGCCGAGCGTAACCA
GGACATCGTGGTCGAGGTGGATGAAAGCCACTCGGTGGGCCATGACCGCAACAAGAGCATCGGCCACAACGAGACGGTGA
CCATCGGCAACAACCGCCTGCGCATCGTCAAGCAGGAGGACATCCTCTCCGTCGGCCAGCGCAAGACCGACAGCATCAGC
CAGAGCTACGTGATTGAAGTGGGCGAAAACCTGCGCCTGGTGTGCGGCGAAAGCATCCTGGAGCTCAACGCCAGCGGCCA
GATCAACCTGACCGGCGTACAGATCAGCTTCTACGCCAGCGGCGATGCCGAGTTCAACACCGGCGGCGTGCTGCACCTGA
ACAACGGCGGCGGCCCCGGAGCCACGCCGGACGGCCAGGGCGTCAAGGGCAGCATCGATGCCAACATCCAAGCCGCGTTC
CCTGCACCCAAGGGCTAA