Detailed information of component protein


Summary

Component ID T6CP005738
Component protein type TssH
T6SS ID (Type) T6SS00540 experimental (Type i3)
Strain Pseudomonas fluorescens F113
Replicon chromosome
Sequence Protein sequence (897 a.a.); Nucleotide sequence (2691 bp)
Reference
[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[2] Redondo-Nieto M, Barret M, Morrissey J, et al. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics. 2013 Jan 25;14:54. doi: 10.1186/1471-2164-14-54. PMID: 23350846
[3] Durán D, Bernal P, Vazquez-Arias D, et al. Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome. Sci Rep. 2021 Mar 11;11(1):5772. doi: 10.1038/s41598-021-85218-1. PMID: 33707614
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSF113_RS59860
Coordinate (Strand) 6689223..6691913 (+)
NCBI ID WP_014340891.1
RefSeq NC_016830
Uniprot ID G8Q724_PSEF3, A0A0Q7A186_9PSED
KEGG ID pfe:PSF113_5806
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 2.4e-38 625..791
AAA_lid_9 PF17871.3 3e-28 373..465
ClpB_D2-small PF10431.11 9e-14 798..872
AAA PF00004.31 5.4e-09 234..367
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-896 MGEISRAALFGKLNSVAYKAIEAATVFCKLRGNPYVELAHWFHQLLQLQDSDLHRIIRQFNIEPARLARDLTEALDRLPRGSTSITDLSSHVEEAVERGWVYGSLMFGESQVRTGYLVLGILKTPSLRHALLGLSSEFDKVKVEALSERFDEYVGDSPENALTASDGFNAGAVPGEASGAMAPSAMGKQEALKRFTVDLTEQARSGKLDPIVGRDEEIRQLVDILMRRRQNNPILTGEAGVGKTAVVEGFALRIVAGDVPPALKDVELRSLDVGLLQAGASMKGEFEQRLRQVIEDVQASPKPIILFIDEAHTLVGAGGAAGTGDAANLLKPALARGTLRTVAATTWAEYKKHIEKDPALTRRFQVVQVAEPSEDKALLMMRGVASTMEKHHQVQILDEALEASVKLSHRYIPARQLPDKSVSLLDTACARVAISLHAVPAEVDDSRRRIEALETELQIIAREHAIGVVIGSRQTQSETLLSAERERLAELEGRWAEEKTLVDELLATRATLRERVGVVDSEDNSEASDNESHQLREKLVDLQQRLTALQGETPLILPTVDYQAVASVVADWTGIPVGRMARNELETVLNLDQHLKKRIIGQDHALQMIAKRIQTSRAGLDNPSKPIGVFMLAGTSGVGKTETALALAEAMYGGEQNVITINMSEFQEAHTVSTLKGAPPGYIGYGEGGVLTEAVRRKPYSVVLLDEVEKAHPDVHEIFFQVFDKGVMEDGEGRVIDFKNTLILLTTNAGTELIAQVCKDPQNVPEPEEIAKALRQPLLEIFPPALLGRLVTIPYYPLSDEMLKAITRLQLNRIKKRVESTHKVAFDYDDAVIDLIVSRCTETESGGRMIDTILTNSLLPDMSREFLTRMLEGKALAGVRISAVDNELQYDFSDAA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9914 - -


  Protein sequence: 897 a.a.    

>T6CP005738 NC_016830:6689223-6691913 [Pseudomonas fluorescens F113] [TssH]
MGEISRAALFGKLNSVAYKAIEAATVFCKLRGNPYVELAHWFHQLLQLQDSDLHRIIRQFNIEPARLARDLTEALDRLPR
GSTSITDLSSHVEEAVERGWVYGSLMFGESQVRTGYLVLGILKTPSLRHALLGLSSEFDKVKVEALSERFDEYVGDSPEN
ALTASDGFNAGAVPGEASGAMAPSAMGKQEALKRFTVDLTEQARSGKLDPIVGRDEEIRQLVDILMRRRQNNPILTGEAG
VGKTAVVEGFALRIVAGDVPPALKDVELRSLDVGLLQAGASMKGEFEQRLRQVIEDVQASPKPIILFIDEAHTLVGAGGA
AGTGDAANLLKPALARGTLRTVAATTWAEYKKHIEKDPALTRRFQVVQVAEPSEDKALLMMRGVASTMEKHHQVQILDEA
LEASVKLSHRYIPARQLPDKSVSLLDTACARVAISLHAVPAEVDDSRRRIEALETELQIIAREHAIGVVIGSRQTQSETL
LSAERERLAELEGRWAEEKTLVDELLATRATLRERVGVVDSEDNSEASDNESHQLREKLVDLQQRLTALQGETPLILPTV
DYQAVASVVADWTGIPVGRMARNELETVLNLDQHLKKRIIGQDHALQMIAKRIQTSRAGLDNPSKPIGVFMLAGTSGVGK
TETALALAEAMYGGEQNVITINMSEFQEAHTVSTLKGAPPGYIGYGEGGVLTEAVRRKPYSVVLLDEVEKAHPDVHEIFF
QVFDKGVMEDGEGRVIDFKNTLILLTTNAGTELIAQVCKDPQNVPEPEEIAKALRQPLLEIFPPALLGRLVTIPYYPLSD
EMLKAITRLQLNRIKKRVESTHKVAFDYDDAVIDLIVSRCTETESGGRMIDTILTNSLLPDMSREFLTRMLEGKALAGVR
ISAVDNELQYDFSDAA*

  Nucleotide sequence: 2691 bp    

>T6CP005738 NC_016830:6689223-6691913 [Pseudomonas fluorescens F113] [TssH]
ATGGGTGAAATCAGTCGCGCCGCGTTGTTCGGCAAACTCAACAGCGTGGCCTACAAAGCCATTGAAGCCGCCACCGTGTT
CTGCAAATTGCGCGGTAACCCTTATGTGGAACTGGCCCACTGGTTTCACCAGTTGCTGCAACTGCAGGACTCGGACCTGC
ACCGCATCATTCGCCAGTTCAACATCGAGCCGGCGCGCCTGGCCCGCGACCTGACCGAAGCCCTGGACCGCCTGCCACGG
GGTTCGACGTCGATCACCGACCTGTCGTCCCACGTGGAAGAAGCCGTGGAACGCGGCTGGGTCTACGGCAGCCTGATGTT
CGGCGAAAGCCAGGTGCGCACCGGTTACCTGGTGCTGGGCATTCTCAAGACCCCGAGCCTGCGCCACGCGCTGCTGGGCC
TGTCGTCGGAGTTCGACAAGGTCAAGGTCGAAGCCCTGAGCGAACGGTTTGACGAATACGTCGGCGACTCGCCGGAAAAC
GCCCTGACTGCCAGCGACGGCTTCAATGCCGGTGCCGTGCCGGGCGAAGCCAGCGGCGCCATGGCCCCTAGCGCCATGGG
CAAACAGGAAGCCCTCAAGCGCTTTACCGTCGACCTTACCGAGCAGGCCCGCAGCGGCAAGCTCGACCCAATCGTCGGCC
GTGACGAAGAGATCCGCCAACTGGTGGACATCCTCATGCGCCGCCGGCAGAACAACCCGATCCTTACCGGTGAAGCCGGC
GTGGGCAAGACCGCCGTGGTCGAAGGCTTCGCCCTGCGCATCGTCGCCGGCGACGTACCACCGGCCCTCAAGGACGTGGA
ACTGCGCAGCCTCGACGTTGGCCTGTTGCAGGCCGGCGCGAGCATGAAAGGCGAGTTCGAACAGCGCCTGCGCCAGGTCA
TCGAAGACGTCCAGGCCTCGCCCAAGCCGATCATCCTGTTCATCGACGAAGCCCACACCCTGGTGGGTGCCGGCGGCGCC
GCCGGCACGGGCGACGCGGCCAACCTGCTCAAGCCGGCGTTGGCCCGCGGCACCTTGCGCACCGTGGCCGCCACGACCTG
GGCCGAGTACAAGAAGCACATCGAAAAAGACCCGGCCCTGACCCGCCGTTTCCAAGTGGTGCAAGTGGCCGAGCCGTCGG
AAGACAAGGCCCTGCTGATGATGCGCGGCGTGGCCTCGACCATGGAAAAACACCACCAGGTGCAGATCCTCGACGAAGCC
CTGGAAGCCTCGGTCAAGCTGTCCCACCGCTACATTCCCGCGCGCCAGCTGCCGGACAAATCCGTGAGCCTGCTGGACAC
CGCTTGCGCCCGCGTCGCCATCAGCCTGCACGCCGTACCGGCCGAAGTGGACGACAGCCGTCGGCGCATCGAAGCGCTGG
AAACCGAGCTGCAAATCATCGCCCGTGAACACGCGATTGGCGTGGTCATCGGCAGCCGCCAGACCCAGAGCGAAACCCTG
CTGAGCGCCGAGCGCGAACGCCTGGCCGAGCTGGAAGGCCGCTGGGCCGAAGAGAAAACCCTGGTGGACGAACTGCTCGC
CACCCGCGCCACCCTGCGCGAGCGCGTTGGCGTGGTGGACAGCGAGGACAACAGCGAAGCTTCTGACAACGAAAGCCACC
AACTGCGTGAGAAACTGGTGGACCTGCAACAGCGCCTGACGGCCCTGCAAGGCGAAACTCCGCTGATCCTGCCGACCGTG
GATTACCAGGCCGTGGCCTCGGTGGTCGCCGACTGGACCGGTATCCCGGTGGGCCGCATGGCCCGCAACGAACTGGAAAC
CGTGCTCAACCTCGACCAGCACCTGAAGAAACGCATCATCGGCCAGGACCATGCCTTGCAGATGATCGCCAAGCGCATCC
AGACCTCCCGCGCCGGCCTCGACAACCCGAGCAAGCCGATTGGTGTGTTCATGCTTGCCGGCACTTCCGGCGTGGGCAAG
ACCGAAACCGCCCTGGCCCTGGCCGAAGCCATGTACGGCGGCGAGCAGAACGTTATCACCATCAACATGAGCGAGTTCCA
GGAAGCCCACACCGTGTCGACCCTCAAGGGCGCGCCACCGGGCTACATCGGCTATGGCGAAGGCGGCGTGCTGACCGAGG
CCGTGCGGCGCAAACCGTACAGCGTGGTGTTGCTGGACGAGGTGGAAAAGGCCCACCCGGACGTGCATGAGATCTTCTTC
CAGGTGTTCGACAAAGGCGTGATGGAGGACGGCGAAGGCCGGGTGATCGACTTCAAGAACACCTTGATCCTGCTGACCAC
CAACGCCGGCACCGAGTTGATCGCCCAGGTCTGCAAAGACCCGCAGAACGTGCCCGAGCCGGAAGAAATCGCCAAGGCCC
TGCGCCAACCGCTGCTGGAGATTTTCCCGCCGGCCTTGCTGGGCCGCCTGGTGACGATCCCGTACTACCCGCTCAGCGAC
GAGATGCTCAAGGCCATCACCCGCCTGCAACTCAACCGCATCAAGAAGCGCGTGGAAAGCACCCACAAAGTCGCCTTCGA
CTACGACGACGCGGTGATCGATTTGATCGTCTCGCGTTGCACCGAAACCGAAAGCGGCGGGCGCATGATCGACACCATCC
TGACCAACAGCCTGCTGCCGGACATGAGCCGCGAGTTCCTGACCCGCATGCTCGAAGGCAAGGCTTTGGCCGGGGTGCGG
ATCAGCGCCGTGGATAACGAATTGCAGTACGATTTCAGCGACGCGGCCTGA