Detailed information of component protein


Summary

Component ID T6CP005737
Component protein type TssG
T6SS ID (Type) T6SS00540 experimental (Type i3)
Strain Pseudomonas fluorescens F113
Replicon chromosome
Sequence Protein sequence (350 a.a.); Nucleotide sequence (1050 bp)
Reference
[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[2] Redondo-Nieto M, Barret M, Morrissey J, et al. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics. 2013 Jan 25;14:54. doi: 10.1186/1471-2164-14-54. PMID: 23350846
[3] Durán D, Bernal P, Vazquez-Arias D, et al. Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome. Sci Rep. 2021 Mar 11;11(1):5772. doi: 10.1038/s41598-021-85218-1. PMID: 33707614
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSF113_RS59855
Coordinate (Strand) 6688181..6689230 (+)
NCBI ID WP_041476205.1
RefSeq NC_016830
Uniprot ID UPI00059CAE91
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssG PF06996.13 1.9e-106 21..318
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-349 MESQARTSSDPVSTLEAMHQEPWEYDFFQALRRIECESPELPRLGHSLRLADDPLRLGQRAECTFAPATLASVQPGSDGAPARLEQFFFGLGGPNGPMPLHITEYVRERQRNNADSTSKRFLDVFHHRLLSLFYRAWAEARPTVSHDRPDDDYWSARLAALSGRGMPSLLNQGLIPDTAKLHYSGHLAAQTRYPDGLKAILGEYFGLPVEIEEYVGQWLELPERSRIGVNAQLGVDFCLGRFVWDRQHKFRIRLGPLKLVDYMGMLPGSQPFNELVAWVAEYLGHELDWDLNLVLEQPEVPALQLNGRFRLGFNTWLGRPETDANDLILARHYAEQANTSQTSRSHEHG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9895 - -


  Protein sequence: 350 a.a.    

>T6CP005737 NC_016830:6688181-6689230 [Pseudomonas fluorescens F113] [TssG]
MESQARTSSDPVSTLEAMHQEPWEYDFFQALRRIECESPELPRLGHSLRLADDPLRLGQRAECTFAPATLASVQPGSDGA
PARLEQFFFGLGGPNGPMPLHITEYVRERQRNNADSTSKRFLDVFHHRLLSLFYRAWAEARPTVSHDRPDDDYWSARLAA
LSGRGMPSLLNQGLIPDTAKLHYSGHLAAQTRYPDGLKAILGEYFGLPVEIEEYVGQWLELPERSRIGVNAQLGVDFCLG
RFVWDRQHKFRIRLGPLKLVDYMGMLPGSQPFNELVAWVAEYLGHELDWDLNLVLEQPEVPALQLNGRFRLGFNTWLGRP
ETDANDLILARHYAEQANTSQTSRSHEHG*

  Nucleotide sequence: 1050 bp    

>T6CP005737 NC_016830:6688181-6689230 [Pseudomonas fluorescens F113] [TssG]
ATGGAAAGCCAAGCCCGGACGTCGTCCGACCCTGTGAGTACCCTGGAGGCGATGCACCAGGAGCCCTGGGAATATGACTT
CTTCCAGGCGCTGCGACGCATCGAGTGCGAATCCCCCGAGCTGCCGCGCCTGGGCCATTCCCTGCGCCTGGCCGACGACC
CGCTGCGCCTCGGGCAACGGGCCGAATGCACCTTCGCCCCGGCCACCCTTGCCTCGGTGCAACCGGGCAGCGATGGCGCG
CCGGCACGCCTGGAGCAATTTTTCTTCGGTCTCGGCGGCCCCAACGGCCCGATGCCGCTGCACATCACCGAATACGTGCG
CGAACGCCAGCGCAACAACGCCGACAGCACCAGCAAGCGCTTCCTCGATGTGTTCCATCATCGCCTGCTCAGCCTGTTTT
ATCGGGCCTGGGCCGAAGCGCGGCCGACGGTCAGCCATGATCGCCCGGACGATGACTATTGGTCGGCGCGCCTGGCCGCC
CTCAGCGGTCGGGGGATGCCGAGCCTGCTCAACCAGGGGTTGATCCCCGACACGGCGAAGCTGCACTACAGCGGCCACCT
GGCGGCGCAAACCCGCTACCCGGACGGTTTGAAGGCAATCCTCGGCGAGTACTTCGGCCTGCCGGTGGAGATCGAAGAAT
ACGTCGGCCAATGGCTGGAACTGCCTGAACGCAGCCGAATCGGTGTCAACGCCCAACTGGGCGTGGACTTCTGCCTGGGC
CGCTTCGTGTGGGATCGCCAGCACAAATTCCGCATTCGCCTGGGGCCACTCAAGCTCGTTGACTACATGGGCATGCTGCC
CGGCAGCCAACCGTTCAACGAGCTGGTGGCCTGGGTCGCCGAATACTTGGGCCATGAACTGGACTGGGACCTGAACCTGG
TCCTGGAGCAACCCGAAGTCCCGGCACTGCAACTCAATGGGCGTTTCCGCCTGGGTTTCAACACCTGGCTGGGCCGTCCC
GAAACAGATGCCAACGATTTAATACTGGCCCGGCATTACGCCGAACAGGCCAACACCTCACAGACCTCAAGGAGCCATGA
GCATGGGTGA