Detailed information of component protein


Summary

Component ID T6CP005736
Component protein type TssF
T6SS ID (Type) T6SS00540 experimental (Type i3)
Strain Pseudomonas fluorescens F113
Replicon chromosome
Sequence Protein sequence (620 a.a.); Nucleotide sequence (1860 bp)
Reference
[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[2] Redondo-Nieto M, Barret M, Morrissey J, et al. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics. 2013 Jan 25;14:54. doi: 10.1186/1471-2164-14-54. PMID: 23350846
[3] Durán D, Bernal P, Vazquez-Arias D, et al. Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome. Sci Rep. 2021 Mar 11;11(1):5772. doi: 10.1038/s41598-021-85218-1. PMID: 33707614
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSF113_RS59850
Coordinate (Strand) 6686358..6688217 (+)
NCBI ID WP_014340889.1
RefSeq NC_016830
Uniprot ID G8Q722_PSEF3
KEGG ID pfe:PSF113_5804
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 2.2e-234 1..616
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-619 MNPRLLELYNQELHHVRESAAEFAKEYPKIASRLTLSGMDCADPYVERLLEGFAYLTARVQLKLDAEYPTFTHNLLEIAYPHYLAPTPSMTVVQLQADPDEGSLSSGFPLPRDTVLRAALGRETQTCCEYRTAHAVTLWPLQVSQAEYFGNPSAVLGRLAASEPKAKAGLRITLRTGAELPFNSLALDNLPLYLSGADEQPFRLYEQLLGNTCAVFARKPGGDWVERLPQDALRSRGFDDADAALPVVPRAFQGYRLLQEYFALPHRFLFVDFTQLSRAVKRCDGQELELIVLFDRHDPSLEGSVGASQFLPFCTPAINLFPKRLDRIHLSDRVNEHHVIADRTRPMDFEVHSLNGLTGHGTGPEQPFLPFYAVRDPSRYGRDQAYYTVRREPRVLSSDQRRNGPRSTYIGSETFVSLVDSQQAPYRHDLRQLGVTALCTNRDLPLFMSVGNGKTDFTLADSAPVGAVRCVAGPSRPRASHAHDAKAWRLISQLSLNYLSLSEQGQGAAALRELLRLYGDSNDAALQLQIEGLREVSSKACTRRLPMPGPIVFGRGLEITLEFDENAFRGTGVFLLGAVFERFLARYVSINSFTETVIRTTERGEIMRWKAKPGRRPTL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9923 - -


  Protein sequence: 620 a.a.    

>T6CP005736 NC_016830:6686358-6688217 [Pseudomonas fluorescens F113] [TssF]
MNPRLLELYNQELHHVRESAAEFAKEYPKIASRLTLSGMDCADPYVERLLEGFAYLTARVQLKLDAEYPTFTHNLLEIAY
PHYLAPTPSMTVVQLQADPDEGSLSSGFPLPRDTVLRAALGRETQTCCEYRTAHAVTLWPLQVSQAEYFGNPSAVLGRLA
ASEPKAKAGLRITLRTGAELPFNSLALDNLPLYLSGADEQPFRLYEQLLGNTCAVFARKPGGDWVERLPQDALRSRGFDD
ADAALPVVPRAFQGYRLLQEYFALPHRFLFVDFTQLSRAVKRCDGQELELIVLFDRHDPSLEGSVGASQFLPFCTPAINL
FPKRLDRIHLSDRVNEHHVIADRTRPMDFEVHSLNGLTGHGTGPEQPFLPFYAVRDPSRYGRDQAYYTVRREPRVLSSDQ
RRNGPRSTYIGSETFVSLVDSQQAPYRHDLRQLGVTALCTNRDLPLFMSVGNGKTDFTLADSAPVGAVRCVAGPSRPRAS
HAHDAKAWRLISQLSLNYLSLSEQGQGAAALRELLRLYGDSNDAALQLQIEGLREVSSKACTRRLPMPGPIVFGRGLEIT
LEFDENAFRGTGVFLLGAVFERFLARYVSINSFTETVIRTTERGEIMRWKAKPGRRPTL*

  Nucleotide sequence: 1860 bp    

>T6CP005736 NC_016830:6686358-6688217 [Pseudomonas fluorescens F113] [TssF]
ATGAACCCACGCCTGCTGGAGCTGTACAACCAGGAACTGCACCACGTGCGCGAAAGCGCGGCGGAGTTCGCCAAGGAATA
CCCGAAGATCGCCAGTCGGCTGACGTTGTCCGGCATGGACTGCGCCGACCCGTATGTCGAGCGCCTGCTGGAAGGCTTCG
CCTACCTGACGGCGCGGGTGCAGCTCAAGCTCGACGCCGAGTACCCGACCTTCACCCACAACCTGCTGGAAATCGCCTAC
CCGCATTACCTGGCGCCGACGCCGTCGATGACCGTGGTGCAATTGCAGGCCGACCCGGACGAAGGCTCGCTGAGCAGTGG
TTTCCCGCTGCCGCGCGATACGGTCCTGCGTGCCGCCCTGGGGCGTGAAACCCAGACCTGCTGCGAGTACCGCACCGCCC
ACGCGGTGACGTTGTGGCCGTTGCAGGTCAGCCAGGCCGAATACTTCGGCAACCCGTCCGCCGTGCTCGGGCGCCTGGCC
GCCAGCGAACCGAAAGCCAAGGCCGGCCTGCGCATCACACTGCGCACCGGCGCCGAGCTGCCGTTCAACAGCCTGGCCCT
GGACAACCTGCCGCTGTACTTGAGCGGTGCCGACGAACAGCCTTTCCGCCTCTACGAACAACTGCTGGGCAATACCTGCG
CAGTGTTCGCCCGCAAGCCCGGCGGCGACTGGGTGGAGCGGCTGCCCCAGGATGCGTTGCGCTCGCGCGGCTTCGACGAT
GCCGACGCCGCGCTGCCGGTGGTGCCGCGGGCCTTCCAGGGCTATCGCCTGTTGCAGGAATACTTCGCCCTGCCCCACCG
TTTCCTGTTTGTCGACTTCACCCAGTTGAGCCGCGCGGTCAAGCGTTGCGACGGCCAGGAGCTGGAGCTGATCGTGCTGT
TCGACCGTCACGACCCAAGCCTGGAAGGCAGCGTCGGCGCTTCGCAGTTCCTGCCGTTCTGCACCCCGGCCATCAACCTG
TTTCCCAAGCGCCTGGACCGCATTCACTTGTCGGATCGGGTCAACGAACACCACGTGATCGCCGACCGTACCCGGCCGAT
GGATTTCGAAGTGCATTCGCTGAACGGCCTCACCGGCCACGGCACCGGGCCGGAGCAGCCGTTCCTGCCGTTCTACGCCG
TGCGCGATCCGTCCCGTTACGGCCGCGACCAGGCCTATTACACCGTGCGCCGCGAACCGCGCGTGCTGTCCAGCGATCAA
CGACGCAACGGCCCGCGCTCGACCTACATCGGCAGCGAAACCTTCGTCAGCCTGGTGGACAGCCAGCAAGCGCCCTATCG
CCACGACCTGCGCCAACTGGGCGTGACCGCGTTGTGCACCAACCGCGACCTGCCGCTGTTCATGAGCGTGGGCAACGGCA
AGACCGACTTCACCCTCGCCGACAGTGCTCCGGTGGGCGCCGTGCGCTGCGTGGCAGGTCCGAGTCGCCCACGGGCCAGC
CACGCCCACGACGCCAAGGCCTGGCGGCTGATCAGCCAGTTGTCACTGAACTACTTGTCGTTGAGCGAACAAGGCCAAGG
CGCGGCCGCCCTGCGTGAACTGCTGCGCCTGTACGGCGACAGCAACGACGCGGCGCTGCAATTGCAGATCGAAGGCCTGC
GCGAAGTCAGCAGCAAGGCCTGCACCCGGCGCCTGCCGATGCCCGGCCCGATCGTGTTTGGTCGAGGCCTGGAAATCACC
CTGGAATTCGATGAAAACGCGTTTCGCGGCACCGGGGTGTTCCTGCTCGGCGCGGTGTTCGAGCGCTTCCTGGCACGCTA
TGTGTCGATCAACAGTTTTACCGAGACGGTGATCCGTACCACCGAACGCGGCGAGATCATGCGATGGAAAGCCAAGCCCG
GACGTCGTCCGACCCTGTGA