Detailed information of component protein


Summary

Component ID T6CP005725
Component protein type TssM
T6SS ID (Type) T6SS00539 experimental (Type i4a)
Strain Pseudomonas fluorescens F113
Replicon chromosome
Sequence Protein sequence (1275 a.a.); Nucleotide sequence (3825 bp)
Reference
[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[2] Redondo-Nieto M, Barret M, Morrissey J, et al. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics. 2013 Jan 25;14:54. doi: 10.1186/1471-2164-14-54. PMID: 23350846
[3] Durán D, Bernal P, Vazquez-Arias D, et al. Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome. Sci Rep. 2021 Mar 11;11(1):5772. doi: 10.1038/s41598-021-85218-1. PMID: 33707614
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSF113_RS42640
Coordinate (Strand) 2821802..2825626 (+)
NCBI ID WP_014337911.1
RefSeq NC_016830
Uniprot ID G8QBI3_PSEF3
KEGG ID pfe:PSF113_2421
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 9e-43 115..350
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-4 MSPL
Transmembrane helix 5-27 SIVALVVLAIVVVLLIAALIWWL
Inside 28-345 RTQGGAAIRSFYGAVRRMEQEQGTRDRYQMPWLMMLGNESDGAQLCAQWRLQPTDKVAWFGRWFCDAEGSVLVVPQALFLPDDGMNRQRGNWWRLLGLMLRLRSKRPLDAVIWTVPFSTLDDIEHTTELSLKVRRCFIDLLQRFGLSLPVYVVITGMEELPGVQELVSALPAEARESMLGWSSPYLPDAAWQSQWSDQALDQVNAALSQSIIEIGALSGQLGTDLYGLPERFEGLRRALQILLEPVFQGNAQGEAPRCRGVYFTASQAPVVSGDGLTAADSAQRQTVFARQLWSRRLVAERGLAQPVPRLLRLRQRGH
Transmembrane helix 346-368 RLVGAVALVVGLVWLGGMVWVWH
Outside 369-1274 DSVEQAEGLSRLILSTHKNHLVVDPDEPQLEPTRHNVQNYWNMLEKAPSWRFVSVVFPTSWFSSVDTQLENGLRLTARHHWILPLRDLLNSDLEQLKSIRNTERRGNVENEDPAQWQSYVKAKELVELAVRLEQHNQMFSQVVNDPKAPLDELVQLSNTALSLGLNTGTLSRPRFYNRVLFDTQNSDLKGLDLNAARPVISDNFTGLMQRWLDHYFLADNFVRQAGYLKLHLQRLEAGSGNSLSELEDLMALIDDLQTLVSLTNSAWGRGKGQDLVPGYSQMLDKVSHSTLLGPDIEQDLESQAAKLQQSFRDQWIVQTGSRDNLLVQQGSGLLVLQDHVTALDGAVQALFKRDFVALAMQKDPSGNNPDSMGQGDGSDDFSVALNYFASYKSYANEEMPRIPPDYRDALMQAAEGAAARAMWLSLDESDEALPGMGFNVQTAQAVALQKAFMDVHRGDLAIRFQRLLNRRALAQLKSGLDEIDAQPLFSQRADVQRWDGSKNFGLQLYGASDPQDLKLNLAQQFGAMLQIAEQRMPALEWLIVQQDNLSALEHDQVARFMALNDELLKYKNQNPASSAAQLEQLVSRDFIAMDTTSCAQILQTSSLPGGRGDLALRTVALQQGALQRCLYLQQNQAAAAWNDLANYFNQYLAGRFPFSQDVRASDADPARVQHLLELIDTRLPLAQAGLTLSQTPERLAAEDFLNRLKQAGTWLGPLFVRDKSGILGVEMDVRWRTDRDEERGADQVIAWSLDAGNQQISYPGGGQPNLRWMVGQPVRLTLRWARNGYQRPANDPLQPNLVVRDLEAGWEYQGPWSLLRLMRSQVASQRQPNVDYTDFPLALQLPVTAQTQARTAQQTLMFVRLSLMSQGSKLPLSIPPLPTRAPRSPFMATASSPAIATREEGL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9980 - -


  Protein sequence: 1275 a.a.    

>T6CP005725 NC_016830:2821802-2825626 [Pseudomonas fluorescens F113] [TssM]
MSPLSIVALVVLAIVVVLLIAALIWWLRTQGGAAIRSFYGAVRRMEQEQGTRDRYQMPWLMMLGNESDGAQLCAQWRLQP
TDKVAWFGRWFCDAEGSVLVVPQALFLPDDGMNRQRGNWWRLLGLMLRLRSKRPLDAVIWTVPFSTLDDIEHTTELSLKV
RRCFIDLLQRFGLSLPVYVVITGMEELPGVQELVSALPAEARESMLGWSSPYLPDAAWQSQWSDQALDQVNAALSQSIIE
IGALSGQLGTDLYGLPERFEGLRRALQILLEPVFQGNAQGEAPRCRGVYFTASQAPVVSGDGLTAADSAQRQTVFARQLW
SRRLVAERGLAQPVPRLLRLRQRGHRLVGAVALVVGLVWLGGMVWVWHDSVEQAEGLSRLILSTHKNHLVVDPDEPQLEP
TRHNVQNYWNMLEKAPSWRFVSVVFPTSWFSSVDTQLENGLRLTARHHWILPLRDLLNSDLEQLKSIRNTERRGNVENED
PAQWQSYVKAKELVELAVRLEQHNQMFSQVVNDPKAPLDELVQLSNTALSLGLNTGTLSRPRFYNRVLFDTQNSDLKGLD
LNAARPVISDNFTGLMQRWLDHYFLADNFVRQAGYLKLHLQRLEAGSGNSLSELEDLMALIDDLQTLVSLTNSAWGRGKG
QDLVPGYSQMLDKVSHSTLLGPDIEQDLESQAAKLQQSFRDQWIVQTGSRDNLLVQQGSGLLVLQDHVTALDGAVQALFK
RDFVALAMQKDPSGNNPDSMGQGDGSDDFSVALNYFASYKSYANEEMPRIPPDYRDALMQAAEGAAARAMWLSLDESDEA
LPGMGFNVQTAQAVALQKAFMDVHRGDLAIRFQRLLNRRALAQLKSGLDEIDAQPLFSQRADVQRWDGSKNFGLQLYGAS
DPQDLKLNLAQQFGAMLQIAEQRMPALEWLIVQQDNLSALEHDQVARFMALNDELLKYKNQNPASSAAQLEQLVSRDFIA
MDTTSCAQILQTSSLPGGRGDLALRTVALQQGALQRCLYLQQNQAAAAWNDLANYFNQYLAGRFPFSQDVRASDADPARV
QHLLELIDTRLPLAQAGLTLSQTPERLAAEDFLNRLKQAGTWLGPLFVRDKSGILGVEMDVRWRTDRDEERGADQVIAWS
LDAGNQQISYPGGGQPNLRWMVGQPVRLTLRWARNGYQRPANDPLQPNLVVRDLEAGWEYQGPWSLLRLMRSQVASQRQP
NVDYTDFPLALQLPVTAQTQARTAQQTLMFVRLSLMSQGSKLPLSIPPLPTRAPRSPFMATASSPAIATREEGL*

  Nucleotide sequence: 3825 bp    

>T6CP005725 NC_016830:2821802-2825626 [Pseudomonas fluorescens F113] [TssM]
ATGAGCCCCCTGAGCATCGTTGCCCTGGTGGTACTCGCCATCGTCGTGGTGCTGTTGATCGCGGCCCTGATCTGGTGGCT
GCGTACCCAGGGCGGGGCGGCGATCCGCAGTTTCTATGGGGCGGTGCGCCGCATGGAGCAGGAGCAGGGCACCCGGGACC
GCTATCAGATGCCCTGGCTGATGATGCTCGGCAACGAAAGCGATGGCGCCCAGTTGTGCGCCCAATGGCGCTTGCAACCG
ACCGACAAGGTGGCCTGGTTCGGGCGCTGGTTTTGCGATGCCGAGGGCTCGGTGCTGGTGGTGCCTCAGGCGTTGTTCCT
GCCCGATGACGGCATGAACCGGCAACGGGGCAACTGGTGGCGCCTGCTGGGGCTGATGTTGCGCTTGCGCAGCAAGCGGC
CATTGGATGCGGTGATCTGGACCGTACCTTTCAGCACCCTTGACGACATCGAGCACACCACCGAGCTGAGCCTCAAGGTC
CGGCGGTGCTTTATCGACCTGTTGCAACGGTTCGGCCTCAGCCTGCCGGTGTACGTGGTGATCACCGGGATGGAAGAGCT
GCCCGGGGTCCAGGAACTGGTCAGCGCGCTGCCCGCCGAGGCGCGTGAATCGATGCTGGGCTGGTCCTCGCCCTATTTGC
CGGATGCGGCCTGGCAGTCGCAGTGGAGTGACCAGGCCCTGGATCAGGTCAACGCGGCGCTGTCGCAGTCGATCATTGAG
ATCGGTGCGCTGTCGGGGCAATTGGGCACCGATCTTTATGGGTTGCCGGAGCGTTTCGAAGGCTTGCGGCGGGCCTTGCA
GATCCTGCTGGAGCCGGTGTTCCAGGGCAATGCCCAGGGCGAAGCACCGCGTTGTCGCGGGGTGTATTTCACTGCCAGCC
AGGCGCCGGTTGTCAGCGGTGATGGTTTGACCGCCGCCGACAGTGCCCAGCGGCAAACCGTGTTCGCCCGGCAATTGTGG
TCACGACGGCTGGTGGCTGAGCGTGGCCTGGCCCAGCCTGTGCCGCGGCTGCTGCGCCTGCGCCAGCGCGGGCATCGGTT
GGTCGGCGCGGTGGCGTTGGTGGTGGGCCTGGTCTGGTTGGGTGGCATGGTCTGGGTCTGGCACGATTCGGTGGAACAAG
CCGAAGGGCTGTCGCGGTTGATCCTCAGCACCCATAAGAACCATCTCGTGGTCGACCCTGACGAACCGCAGCTGGAACCG
ACCCGACACAACGTGCAGAACTACTGGAACATGTTGGAAAAGGCCCCGAGCTGGCGCTTCGTCTCGGTGGTCTTTCCTAC
TTCGTGGTTCTCTTCCGTGGACACGCAACTGGAGAATGGGTTGCGCCTGACGGCTCGGCACCATTGGATATTGCCCTTGC
GGGACCTGCTCAATTCCGACCTGGAACAGCTCAAGTCCATCCGCAACACTGAGCGGCGGGGCAATGTGGAGAACGAAGAC
CCGGCGCAATGGCAGAGTTATGTGAAGGCCAAGGAACTGGTGGAGCTCGCGGTACGCCTTGAGCAGCACAACCAGATGTT
CAGCCAGGTGGTGAACGATCCCAAGGCACCGCTGGACGAACTGGTGCAACTGAGCAACACCGCCTTGTCCCTGGGTCTCA
ACACCGGCACCTTGAGCCGGCCGCGGTTCTACAACCGGGTGCTGTTCGATACCCAGAACAGCGATTTGAAAGGGCTGGAC
CTGAACGCCGCGCGCCCGGTGATCTCCGATAACTTCACCGGCCTGATGCAGCGCTGGCTGGACCATTACTTCCTGGCGGA
CAACTTCGTGCGCCAGGCCGGTTACCTCAAGCTGCACCTGCAACGGCTGGAGGCCGGCAGTGGCAATTCGTTGAGCGAAC
TCGAGGACCTGATGGCGCTGATCGACGACTTGCAGACGCTGGTCAGCCTCACCAATTCGGCCTGGGGCCGGGGCAAGGGC
CAGGACCTGGTACCCGGCTACAGCCAGATGCTGGACAAGGTCAGCCACAGCACGTTGTTGGGGCCGGATATCGAGCAGGA
CCTGGAGAGCCAGGCGGCGAAGTTGCAGCAGAGCTTTCGTGACCAGTGGATCGTCCAGACCGGTTCACGGGACAATCTGC
TGGTGCAGCAGGGCAGTGGCCTGCTGGTTTTGCAAGACCATGTCACGGCGCTGGACGGTGCGGTGCAGGCGCTGTTCAAG
CGTGATTTCGTGGCGTTGGCCATGCAGAAGGATCCGTCGGGCAATAACCCCGACTCGATGGGCCAGGGCGATGGCAGCGA
TGATTTCAGCGTCGCCCTGAACTACTTCGCCAGCTACAAGAGCTATGCCAATGAAGAAATGCCGCGCATTCCGCCGGATT
ACCGTGATGCGCTGATGCAGGCCGCCGAAGGTGCTGCGGCCCGGGCCATGTGGTTGAGCCTGGATGAAAGCGATGAGGCA
TTGCCCGGTATGGGCTTCAATGTACAAACGGCCCAGGCCGTTGCGCTGCAAAAAGCCTTCATGGACGTGCACCGTGGCGA
CCTGGCGATTCGTTTCCAACGTCTGCTCAATCGCCGGGCATTGGCGCAACTCAAGAGTGGCCTGGACGAAATCGATGCCC
AGCCGCTGTTCAGCCAGCGGGCCGATGTCCAGCGCTGGGACGGTTCGAAGAACTTCGGCTTGCAACTCTACGGTGCCAGC
GATCCACAGGATCTGAAGCTGAACCTGGCCCAGCAATTCGGCGCGATGCTGCAGATCGCCGAGCAGCGAATGCCCGCGCT
GGAATGGTTGATCGTCCAGCAGGACAACCTGTCGGCCCTGGAGCATGACCAGGTGGCACGCTTCATGGCCCTCAACGATG
AATTGCTCAAGTACAAGAACCAGAACCCGGCCAGCTCGGCGGCCCAATTGGAACAATTGGTCAGCCGGGACTTTATCGCC
ATGGACACCACTTCCTGCGCGCAGATCTTGCAGACCTCCAGCCTGCCCGGCGGGCGCGGTGACCTGGCCCTGCGCACCGT
CGCGTTGCAGCAGGGCGCCTTGCAACGTTGCTTGTACCTGCAACAAAACCAGGCCGCGGCCGCCTGGAATGACCTGGCCA
ACTATTTCAATCAATACCTGGCCGGGCGCTTTCCGTTTTCCCAGGACGTGCGGGCCAGCGATGCCGACCCGGCGCGGGTC
CAGCATTTGCTGGAGCTGATCGACACACGCCTGCCCTTGGCCCAGGCCGGGCTGACGTTGAGCCAGACGCCAGAGCGGCT
GGCGGCCGAGGATTTCCTCAACCGTTTGAAACAGGCCGGTACCTGGCTTGGGCCGCTGTTCGTGCGCGACAAGAGTGGCA
TCCTTGGGGTGGAAATGGACGTGCGCTGGCGCACCGATCGCGATGAAGAGCGCGGTGCCGACCAGGTGATCGCCTGGAGC
CTGGACGCCGGCAACCAACAGATCAGCTACCCCGGCGGCGGCCAGCCGAACCTGCGCTGGATGGTCGGCCAGCCTGTACG
CTTGACCTTGCGCTGGGCCCGCAACGGCTATCAGCGTCCGGCCAACGACCCGCTGCAACCGAACCTGGTGGTGCGCGACC
TGGAGGCCGGTTGGGAGTACCAGGGGCCTTGGTCATTGCTGCGCCTGATGCGCTCGCAGGTGGCGAGTCAGCGCCAGCCG
AATGTCGACTACACCGATTTCCCCCTGGCCCTGCAACTTCCGGTGACCGCTCAGACCCAGGCCCGCACGGCGCAGCAAAC
GTTGATGTTCGTGCGCCTGTCGTTGATGAGCCAGGGCTCCAAGCTGCCCCTGTCGATCCCGCCACTGCCGACGCGGGCGC
CCCGTTCACCTTTCATGGCGACCGCTTCGAGCCCCGCCATTGCCACTCGTGAGGAGGGCCTGTGA