Detailed information of component protein


Summary

Component ID T6CP005724
Component protein type TssK
T6SS ID (Type) T6SS00539 experimental (Type i4a)
Strain Pseudomonas fluorescens F113
Replicon chromosome
Sequence Protein sequence (444 a.a.); Nucleotide sequence (1332 bp)
Reference
[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[2] Redondo-Nieto M, Barret M, Morrissey J, et al. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics. 2013 Jan 25;14:54. doi: 10.1186/1471-2164-14-54. PMID: 23350846
[3] DurĂ¡n D, Bernal P, Vazquez-Arias D, et al. Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome. Sci Rep. 2021 Mar 11;11(1):5772. doi: 10.1038/s41598-021-85218-1. PMID: 33707614
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) PSF113_RS42630
Coordinate (Strand) 2819691..2821022 (+)
NCBI ID WP_014337909.1
RefSeq NC_016830
Uniprot ID G8QBI1_PSEO1
KEGG ID pfe:PSF113_2419
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasE PF05936.14 3.7e-37 20..386

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-443 MSLLPDAVCWHEGMQLLPQHFQLQGLRAEALAAYFAGACNPWFWGVSQLEVDPSALSAGQVRLLQLQGTLPDGLPVNLQAGVGPTLDLDITEAIDKTDDATVTVYLAVSPLWRAGQLVPLKGRLQSVVGEALPDLTSGAFPESITVWRPNPRLCTQLSKADSICLPLLRIRKEGGGFWRVPYTPPTPILLPESALGRRVAGVCARAREKCMFLAGRLRQAQAAGNQDDAMEIRRQLTALWARLPEVEGALNTRVATPQALYGLLLGLAGSWSALDPLAGVPAFAPLDFLELQRGYEPLLDWLETTLELVRAGYRSLAFERDEQVFSIQLPDDEPDQRLVIGLRMPNGASEQAATDWLRGAIIASARHIPLLTRQRMSGLPHQAMSRNEQVAYSVGEDTRLFVVAASGQWFDAQLPLHIVAPASAQASSPWQVVLFVAQASESA



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9803 - -



  Protein sequence: 444 a.a.    

>T6CP005724 NC_016830:2819691-2821022 [Pseudomonas fluorescens F113] [TssK]
MSLLPDAVCWHEGMQLLPQHFQLQGLRAEALAAYFAGACNPWFWGVSQLEVDPSALSAGQVRLLQLQGTLPDGLPVNLQA
GVGPTLDLDITEAIDKTDDATVTVYLAVSPLWRAGQLVPLKGRLQSVVGEALPDLTSGAFPESITVWRPNPRLCTQLSKA
DSICLPLLRIRKEGGGFWRVPYTPPTPILLPESALGRRVAGVCARAREKCMFLAGRLRQAQAAGNQDDAMEIRRQLTALW
ARLPEVEGALNTRVATPQALYGLLLGLAGSWSALDPLAGVPAFAPLDFLELQRGYEPLLDWLETTLELVRAGYRSLAFER
DEQVFSIQLPDDEPDQRLVIGLRMPNGASEQAATDWLRGAIIASARHIPLLTRQRMSGLPHQAMSRNEQVAYSVGEDTRL
FVVAASGQWFDAQLPLHIVAPASAQASSPWQVVLFVAQASESA*

  Nucleotide sequence: 1332 bp    

>T6CP005724 NC_016830:2819691-2821022 [Pseudomonas fluorescens F113] [TssK]
ATGAGTCTGTTACCTGACGCGGTGTGCTGGCATGAGGGCATGCAATTGCTGCCCCAGCATTTTCAATTGCAAGGGCTGCG
TGCCGAGGCGCTGGCCGCGTATTTTGCCGGGGCCTGCAACCCTTGGTTCTGGGGCGTAAGCCAACTGGAAGTCGACCCTT
CGGCCCTCAGCGCCGGCCAGGTGCGGCTGCTGCAACTGCAAGGCACCTTGCCGGACGGCTTGCCGGTCAACCTGCAGGCC
GGCGTCGGGCCGACCTTGGATCTGGACATCACCGAGGCGATCGACAAGACCGACGATGCCACTGTGACTGTGTACCTGGC
CGTCAGCCCACTGTGGCGCGCCGGGCAATTGGTGCCGCTCAAGGGGCGCCTGCAATCGGTGGTCGGCGAGGCGTTACCAG
ACCTCACCAGCGGTGCATTTCCCGAGTCGATCACCGTCTGGCGACCCAACCCGAGGCTGTGCACGCAACTGAGCAAGGCC
GATTCGATCTGCCTGCCGCTGTTGCGCATCCGCAAGGAGGGCGGTGGTTTCTGGCGCGTGCCCTACACCCCACCGACGCC
GATCCTGTTGCCGGAATCGGCCCTCGGTCGACGGGTCGCCGGGGTGTGCGCCCGGGCCCGGGAAAAGTGCATGTTTCTCG
CCGGTCGCTTGCGTCAGGCCCAGGCGGCTGGCAACCAGGACGATGCCATGGAGATCCGCCGGCAGTTGACCGCCTTGTGG
GCGCGCTTGCCGGAAGTCGAAGGGGCGCTGAACACCCGGGTGGCCACGCCCCAGGCGCTCTACGGTTTGCTGCTGGGGCT
GGCTGGAAGCTGGTCGGCCCTCGATCCGCTGGCGGGCGTCCCGGCCTTCGCGCCGCTGGACTTCCTCGAATTGCAGCGCG
GCTATGAACCGTTGCTCGACTGGCTGGAAACCACCCTGGAACTGGTTCGCGCCGGCTATCGCAGCCTGGCCTTCGAGCGC
GATGAGCAGGTCTTCTCGATCCAGTTGCCTGACGACGAGCCGGACCAACGCCTGGTGATCGGCCTGCGCATGCCCAACGG
CGCCAGTGAGCAGGCCGCAACCGATTGGTTGCGCGGGGCGATCATCGCGTCGGCGCGGCACATCCCGCTGCTGACCCGAC
AACGCATGAGCGGTCTGCCCCATCAGGCCATGAGCCGCAACGAACAGGTGGCCTACAGCGTGGGCGAGGACACCCGTTTG
TTTGTGGTGGCCGCCAGCGGGCAATGGTTCGACGCGCAATTGCCGCTGCACATCGTGGCGCCGGCATCGGCCCAGGCCAG
CAGCCCGTGGCAAGTGGTGTTGTTCGTGGCGCAAGCCAGTGAAAGTGCCTGA