Detailed information of component protein


Summary

Component ID T6CP005723
Component protein type TssB
T6SS ID (Type) T6SS00539 experimental (Type i4a)
Strain Pseudomonas fluorescens F113
Replicon chromosome
Sequence Protein sequence (187 a.a.); Nucleotide sequence (561 bp)
Reference
[1] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[2] Redondo-Nieto M, Barret M, Morrissey J, et al. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics. 2013 Jan 25;14:54. doi: 10.1186/1471-2164-14-54. PMID: 23350846
[3] Durán D, Bernal P, Vazquez-Arias D, et al. Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome. Sci Rep. 2021 Mar 11;11(1):5772. doi: 10.1038/s41598-021-85218-1. PMID: 33707614
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSF113_RS42620
Coordinate (Strand) 2818392..2818952 (-)
NCBI ID WP_014337907.1
RefSeq NC_016830
Uniprot ID G8QBH9_PSEF3, A0A0F4XMB4_9PSED
KEGG ID pfe:PSF113_2417
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VipA PF05591.14 1.8e-55 10..160
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-186 MAESTQHKLDRVRPPRVQITYDVEIGNAIEKKELPLVVGILADLSGKPLEPLPKLNERRFTEIDRDNFNEVLASISPRATLQVNNTLSGDDSKLNIELNFHHIDDFDPVKVVEQVTPLRRLFEARQRLRDLLTKLDGNDDLDKLLRDVIANTEGLQEIKSARPQDVPPELPAVAANDDPAPAEPQA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9976 - -


  Protein sequence: 187 a.a.    

>T6CP005723 NC_016830:c2818952-2818392 [Pseudomonas fluorescens F113] [TssB]
MAESTQHKLDRVRPPRVQITYDVEIGNAIEKKELPLVVGILADLSGKPLEPLPKLNERRFTEIDRDNFNEVLASISPRAT
LQVNNTLSGDDSKLNIELNFHHIDDFDPVKVVEQVTPLRRLFEARQRLRDLLTKLDGNDDLDKLLRDVIANTEGLQEIKS
ARPQDVPPELPAVAANDDPAPAEPQA*

  Nucleotide sequence: 561 bp    

>T6CP005723 NC_016830:c2818952-2818392 [Pseudomonas fluorescens F113] [TssB]
ATGGCAGAAAGTACCCAGCACAAGCTCGACAGAGTCCGTCCTCCCCGCGTCCAGATCACCTATGACGTGGAAATCGGCAA
CGCCATCGAGAAAAAAGAATTGCCACTGGTGGTCGGCATTCTGGCCGACCTCTCAGGCAAGCCACTGGAGCCGCTGCCCA
AGCTCAACGAGCGGCGCTTCACGGAAATCGATCGGGATAATTTCAACGAGGTACTCGCGTCCATCTCCCCCCGCGCCACG
CTCCAGGTGAACAACACCCTCAGCGGCGACGACAGCAAGCTCAACATCGAACTCAACTTCCATCACATCGACGACTTCGA
CCCGGTCAAGGTCGTGGAGCAGGTCACGCCGCTGCGCCGGTTGTTCGAAGCGCGCCAACGCCTGCGGGACCTGTTGACCA
AACTCGATGGCAACGACGACCTGGACAAGCTGCTGCGCGACGTGATCGCCAACACCGAAGGCCTTCAGGAAATCAAGTCG
GCGCGCCCCCAGGACGTACCGCCAGAGCTGCCCGCCGTGGCCGCCAACGATGACCCTGCCCCGGCCGAACCACAGGCCTG
A