Detailed information of component protein


Summary

Component ID T6CP000349
Component protein type TssH
T6SS ID (Type) T6SS00042 experimental (Type i1)
Strain Pseudomonas syringae pv. tomato str. DC3000
Replicon chromosome
Sequence Protein sequence (883 a.a.); Nucleotide sequence (2649 bp)
Reference
[1] Haapalainen M, Mosorin H, Dorati F, et al. Hcp2, a secreted protein of the phytopathogen Pseudomonas syringae pv. tomato DC3000, is required for fitness for competition against bacteria and yeasts. J Bacteriol. 2012 Sep;194(18):4810-22. doi: 10.1128/JB.00611-12. Epub 2012 Jun 29. PMID: 22753062
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSPTO_RS28065
Coordinate (Strand) 6166575..6169223 (-)
NCBI ID WP_011105486.1
RefSeq NC_004578
Uniprot ID Q87U82_PSESM
KEGG ID pst:PSPTO_5425
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 2.4e-42 616..781
AAA_lid_9 PF17871.3 1.6e-28 348..440
AAA PF00004.31 2.8e-10 208..339
ClpB_D2-small PF10431.11 8.4e-10 792..859
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-882 MSNVDLQQLIQALDAETRRDLESSAERCVARGGSKILVEDLLLELLERPQGLLARALQDAEVDAGELSASLQSRVEHSASRNPVFAPELVQWLQDALLVANLELGQTQVEQAALILALLRNPMRYAGSRYQPLLAKLNIDRLKEFALSKQEQPANGKSAAQGESLLERFTHNLTQQARDGKLDPVLCRDGAIRQMVDILARRRKNNPIVVGEAGVGKTAIVEGLASRIAAGEVPQALKGVELLSLDMGLLQAGASVKGEFERRLKGVIDEVKASPKPIILFIDEAHTLIGAGGNAGGSDAANLLKPALARGELRTIAATTWAEYKKYFEKDPALARRFQPVQLHEPTVSEAVTILRGLAQVYEKSHGIYLRDDAVVAAAELSARYLAGRQLPDKAVDVLDTACARVRISLAAAPESLERLRGELAEGGRQRQALRRDAEAGLLIDHESLEALETRLHAAEEERVALEAMWLEQKTLAERLLELRQQLAKAREAVAAVPVVEIGEDDEGTVIEAVALDETQSVEALTAALNDTHVALAALQVKERLVSFEVCPRLVAEVISAWTGVPLAQLAREHNAKVASFAKDLRIRIRGQEQAVHALDRSMRATAAGLNKPDAPVGVFLLVGPSGVGKTETALALADLLYGGDRFITTINMSEFQEKHTVSRLIGAPPGYVGYGEGGMLTEAVRQKPYSVVLLDEVEKADPDVLNLFYQIFDKGVANDGEGREIDFRNTLILMTSNLGSDQISALCEDGARPTAEVLEETIRPVLSKHFKPALLARMRVVPYYPVSGPVLRELIEIKLGRLGERLNRRQLEFTYCQDLVDHLAQRCTQSDSGARLIDHLLDLHVLPLVADRLLDAMATGESLKRVHATLDTSGTVTCEFA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9935 - -


  Protein sequence: 883 a.a.    

>T6CP000349 NC_004578:c6169223-6166575 [Pseudomonas syringae pv. tomato str. DC3000] [TssH]
MSNVDLQQLIQALDAETRRDLESSAERCVARGGSKILVEDLLLELLERPQGLLARALQDAEVDAGELSASLQSRVEHSAS
RNPVFAPELVQWLQDALLVANLELGQTQVEQAALILALLRNPMRYAGSRYQPLLAKLNIDRLKEFALSKQEQPANGKSAA
QGESLLERFTHNLTQQARDGKLDPVLCRDGAIRQMVDILARRRKNNPIVVGEAGVGKTAIVEGLASRIAAGEVPQALKGV
ELLSLDMGLLQAGASVKGEFERRLKGVIDEVKASPKPIILFIDEAHTLIGAGGNAGGSDAANLLKPALARGELRTIAATT
WAEYKKYFEKDPALARRFQPVQLHEPTVSEAVTILRGLAQVYEKSHGIYLRDDAVVAAAELSARYLAGRQLPDKAVDVLD
TACARVRISLAAAPESLERLRGELAEGGRQRQALRRDAEAGLLIDHESLEALETRLHAAEEERVALEAMWLEQKTLAERL
LELRQQLAKAREAVAAVPVVEIGEDDEGTVIEAVALDETQSVEALTAALNDTHVALAALQVKERLVSFEVCPRLVAEVIS
AWTGVPLAQLAREHNAKVASFAKDLRIRIRGQEQAVHALDRSMRATAAGLNKPDAPVGVFLLVGPSGVGKTETALALADL
LYGGDRFITTINMSEFQEKHTVSRLIGAPPGYVGYGEGGMLTEAVRQKPYSVVLLDEVEKADPDVLNLFYQIFDKGVAND
GEGREIDFRNTLILMTSNLGSDQISALCEDGARPTAEVLEETIRPVLSKHFKPALLARMRVVPYYPVSGPVLRELIEIKL
GRLGERLNRRQLEFTYCQDLVDHLAQRCTQSDSGARLIDHLLDLHVLPLVADRLLDAMATGESLKRVHATLDTSGTVTCE
FA*

  Nucleotide sequence: 2649 bp    

>T6CP000349 NC_004578:c6169223-6166575 [Pseudomonas syringae pv. tomato str. DC3000] [TssH]
ATGAGCAATGTAGACCTGCAACAACTTATTCAAGCGCTGGACGCCGAAACCCGTCGTGATCTGGAAAGCTCCGCGGAGCG
CTGCGTAGCCCGGGGCGGTAGCAAGATTCTGGTTGAAGATCTGTTGCTGGAGCTGCTGGAGCGTCCGCAAGGTCTGCTCG
CGCGTGCCCTGCAGGATGCTGAAGTGGACGCTGGTGAATTGAGTGCCTCTCTGCAATCGCGGGTCGAGCACAGCGCTTCA
CGCAACCCGGTGTTTGCTCCGGAACTGGTGCAATGGCTGCAGGATGCCTTGCTGGTGGCCAACCTTGAACTGGGTCAGAC
CCAGGTCGAACAGGCCGCGCTGATCCTCGCACTACTGCGCAACCCGATGCGCTACGCCGGTAGCCGCTATCAGCCTTTGC
TGGCCAAACTGAATATTGATCGGCTGAAAGAGTTTGCTCTGTCCAAGCAAGAGCAGCCTGCCAACGGCAAATCGGCTGCT
CAGGGCGAATCGCTGTTGGAGCGTTTCACTCACAACCTGACCCAGCAGGCCCGCGACGGCAAGCTTGATCCGGTACTGTG
CCGCGATGGCGCGATTCGGCAAATGGTCGACATCCTGGCTCGGCGCCGCAAGAACAATCCGATTGTGGTCGGCGAAGCCG
GTGTCGGTAAAACCGCCATCGTTGAAGGTCTCGCATCCCGAATTGCTGCGGGTGAAGTGCCGCAGGCGCTCAAGGGCGTC
GAGCTGCTGTCGCTGGACATGGGGCTGCTGCAGGCTGGCGCCAGCGTAAAGGGTGAGTTCGAGCGTCGCCTCAAAGGTGT
GATCGACGAAGTCAAAGCGTCGCCTAAGCCGATCATTCTGTTCATCGATGAAGCCCACACGCTTATCGGTGCTGGCGGCA
ACGCTGGTGGTTCCGATGCCGCCAACCTGCTCAAGCCGGCTCTGGCGCGTGGCGAGTTGCGCACCATCGCTGCCACGACG
TGGGCTGAGTACAAGAAGTACTTTGAAAAAGACCCGGCTCTGGCGCGTCGCTTCCAGCCTGTTCAACTGCATGAACCGAC
CGTAAGCGAAGCCGTGACCATCCTGCGTGGTCTGGCACAAGTCTATGAGAAGAGCCATGGCATTTACTTGCGCGACGACG
CTGTGGTTGCTGCCGCCGAGCTGTCTGCCCGCTATCTGGCTGGCCGCCAGCTTCCTGACAAAGCAGTCGATGTTCTCGAT
ACGGCGTGCGCGCGCGTGCGTATCAGCCTGGCTGCCGCGCCGGAGAGTCTGGAGCGCCTGCGTGGCGAGCTGGCTGAAGG
TGGTCGCCAGCGTCAGGCATTGCGTCGCGACGCTGAAGCCGGTCTGCTGATTGACCACGAGTCGCTGGAAGCTCTGGAAA
CGCGTCTGCACGCAGCTGAAGAGGAAAGGGTTGCACTGGAAGCAATGTGGCTCGAACAGAAAACATTGGCCGAACGCTTG
CTGGAACTGCGTCAGCAACTGGCCAAGGCGCGTGAAGCGGTAGCTGCCGTGCCAGTCGTTGAAATCGGAGAGGACGACGA
GGGCACGGTCATCGAAGCCGTTGCGCTGGATGAAACGCAGAGCGTAGAGGCGCTGACAGCAGCCCTGAACGATACCCATG
TCGCCCTGGCCGCACTGCAGGTCAAGGAGCGACTGGTGAGCTTCGAGGTCTGTCCGCGTCTTGTGGCTGAGGTGATCAGC
GCCTGGACCGGTGTCCCGCTGGCGCAACTGGCTCGTGAGCACAACGCCAAGGTTGCCAGTTTCGCCAAGGACCTTCGTAT
TCGCATCCGTGGTCAAGAGCAGGCCGTTCATGCCCTTGATCGCTCCATGCGCGCTACTGCCGCTGGCTTGAACAAACCCG
ATGCGCCGGTAGGCGTGTTTCTGCTTGTGGGGCCGAGCGGCGTTGGCAAGACTGAAACGGCGCTGGCGCTCGCTGATCTG
TTGTACGGAGGTGATCGTTTTATCACCACCATCAACATGTCCGAGTTTCAGGAGAAGCATACGGTTTCGCGCCTGATCGG
TGCGCCGCCAGGTTACGTCGGTTATGGCGAAGGCGGCATGCTCACCGAAGCTGTACGTCAGAAGCCGTATTCGGTCGTGC
TGCTTGATGAAGTCGAGAAAGCCGACCCTGATGTGCTCAACCTGTTCTATCAGATCTTCGACAAAGGTGTGGCCAACGAC
GGCGAGGGACGCGAGATCGACTTCCGCAATACGCTGATTCTGATGACATCGAACCTTGGCAGCGACCAGATCAGCGCACT
GTGCGAAGACGGCGCGCGGCCAACCGCCGAAGTGCTTGAGGAGACCATCCGGCCGGTCCTCAGCAAACACTTCAAGCCAG
CGTTACTGGCACGTATGCGCGTAGTGCCGTACTACCCGGTCAGCGGGCCGGTGCTACGCGAGCTGATCGAAATCAAACTC
GGTCGCCTGGGCGAGCGCCTGAACCGTCGTCAGCTGGAATTCACTTACTGTCAGGACCTCGTTGATCACTTGGCGCAGCG
TTGCACTCAAAGCGACAGCGGTGCGCGTCTGATCGACCATCTGCTGGACCTTCATGTCCTGCCGCTGGTAGCTGACCGCC
TGCTCGATGCTATGGCTACCGGAGAAAGTCTGAAGCGTGTTCATGCCACGCTGGACACTTCCGGCACCGTGACGTGTGAG
TTTGCCTGA