Detailed information of component protein


Summary

Component ID T6CP000348
Component protein type TssJ
T6SS ID (Type) T6SS00042 experimental (Type i1)
Strain Pseudomonas syringae pv. tomato str. DC3000
Replicon chromosome
Sequence Protein sequence (167 a.a.); Nucleotide sequence (501 bp)
Reference
[1] Haapalainen M, Mosorin H, Dorati F, et al. Hcp2, a secreted protein of the phytopathogen Pseudomonas syringae pv. tomato DC3000, is required for fitness for competition against bacteria and yeasts. J Bacteriol. 2012 Sep;194(18):4810-22. doi: 10.1128/JB.00611-12. Epub 2012 Jun 29. PMID: 22753062
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSPTO_RS28045
Coordinate (Strand) 6163099..6163599 (-)
NCBI ID WP_011105482.1
RefSeq NC_004578
Uniprot ID Q87U86_PSESM
KEGG ID pst:PSPTO_5421
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 7.2e-41 34..151
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MSRRST
Transmembrane helix 7-29 AFFNMLAALTALLVLAGCSTLSP
Outside 30-166 YSNVTKLNLKLTASDQVNPDLNGRPSPVVVRLFELKHPVAFENADFFSLYERAKESLAPDMVASEELELRPGETVELKLSLQEGSRYVGVLAAYRDLPDSRWRYTLQVTPVGITDADLTLDQNGIRNTHEPLAKADD


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9964 pos: 23-24. LAG-CS. MSRRSTAFFNMLAALTALLVLAG


  Protein sequence: 167 a.a.    

>T6CP000348 NC_004578:c6163599-6163099 [Pseudomonas syringae pv. tomato str. DC3000] [TssJ]
MSRRSTAFFNMLAALTALLVLAGCSTLSPYSNVTKLNLKLTASDQVNPDLNGRPSPVVVRLFELKHPVAFENADFFSLYE
RAKESLAPDMVASEELELRPGETVELKLSLQEGSRYVGVLAAYRDLPDSRWRYTLQVTPVGITDADLTLDQNGIRNTHEP
LAKADD*

  Nucleotide sequence: 501 bp    

>T6CP000348 NC_004578:c6163599-6163099 [Pseudomonas syringae pv. tomato str. DC3000] [TssJ]
ATGTCTCGCCGCTCGACTGCTTTCTTTAACATGCTGGCTGCGCTCACCGCCTTGTTGGTGCTGGCCGGCTGCTCTACGTT
GTCGCCGTACTCAAACGTCACCAAGCTCAACCTGAAACTGACCGCCAGCGACCAGGTGAACCCGGACCTCAACGGTCGCC
CGTCCCCCGTTGTTGTGCGCCTGTTCGAGCTCAAGCACCCGGTAGCGTTCGAAAATGCAGATTTCTTCAGCTTGTATGAG
CGCGCAAAAGAATCTCTGGCCCCGGACATGGTTGCGTCTGAAGAGCTGGAGCTGCGTCCGGGTGAAACCGTCGAGCTCAA
GCTCAGCCTGCAAGAAGGCAGTCGTTACGTCGGCGTTCTTGCAGCCTATCGGGACCTGCCGGACAGCAGATGGCGCTACA
CGCTGCAGGTAACACCGGTAGGTATCACTGACGCTGACCTGACTCTTGATCAGAACGGCATTCGTAACACCCATGAACCG
CTCGCCAAGGCAGATGACTGA