Detailed information of component protein


Summary

Component ID T6CP000275
Component protein type TssM
T6SS ID (Type) T6SS00035 experimental (Type i3)
Strain Pseudomonas protegens Pf-5
Replicon chromosome
Sequence Protein sequence (1169 a.a.); Nucleotide sequence (3507 bp)
Reference
[1] Loper JE, Hassan KA, Mavrodi DV, et al. Comparative genomics of plant-associated Pseudomonas spp.: insights into diversity and inheritance of traits involved in multitrophic interactions. PLoS Genet. 2012 Jul;8(7):e1002784. doi: 10.1371/journal.pgen.1002784. Epub 2012 Jul 5. PMID: 22792073
[2] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[3] Hassan KA, Johnson A, Shaffer BT, et al. Inactivation of the GacA response regulator in Pseudomonas fluorescens Pf-5 has far-reaching transcriptomic consequences. Environ Microbiol. 2010 Apr;12(4):899-915. doi: 10.1111/j.1462-2920.2009.02134.x. Epub 2010 Jan 18. PMID: 20089046
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[5] Whitney JC, Chou S, Russell AB, et al. Identification, structure, and function of a novel type VI secretion peptidoglycan glycoside hydrolase effector-immunity pair. J Biol Chem. 2013 Sep 13;288(37):26616-24. doi: 10.1074/jbc.M113.488320. Epub 2013 Jul 22. PMID: 23878199
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PFL_RS30825
Coordinate (Strand) 6904309..6907815 (-)
NCBI ID WP_011064249.1
RefSeq NC_004129
Uniprot ID Q4K3P4_PSEF5
KEGG ID pfl:PFL_6081
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 3e-98 190..447
IcmF-related PF06761.14 4.6e-83 500..792
IcmF_C PF06744.14 6.9e-29 1043..1149
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 VKAFFS
Transmembrane helix 7-29 FVIRWVIPLLGLIALSLIIWFIG
Outside 30-38 PLLEFLVPE
Transmembrane helix 39-61 GRRWALIIFIFALWIGYRVFCII
Inside 62-439 QARRNAAKVLQSLAADTPPDPDSVATAEELAALRQRMDEALALLKKAKLGGDERRNLYELPWYVIIGPPGSGKTTALVNSGLHFPLAAQLGAGAVRGVGGTRNCDWWFTDQAVLLDTAGRYTTQDSHATVDKAAWLGFLDLLKTQRSRRPIDGAFVAISLSDLLLGSEAERAAHAAAIRLRIQELYTQLGVRFPIYLMLTKLDLVPGFMEFFDSLSKEERAQVWGMTFALDDGKSGDSPLAHLPAELAALEQRLNERLVERLQQERDPARRDLIYGFPQQFAALKESLQSFLEGVFKPNAFEERVLLRGVYFTSGTQEGSPIDRLIGAMAQSMNLDRQHLARQTGTGRSYFIEKLFSAVAFAERGLVGVNPKVERRRK
Transmembrane helix 440-462 WIARGALAGTVALVLVVSTLWII
Outside 463-1168 SYRANQAYIAQVDQRVGPVRQDVQNLSPAQRDVLAVLPLLNATRHLAGDAPGWAEGLGLYQGDMLEAESASVYRKLLVAVFAPRLMTRIEEQLHSGGNSDFLYEGLKAYLMLADSEHYDPDFIKAWITLDWDHTLARDLPPEQRQALTGHLQALFEKHPPNARLDQRLIDDLRRQLQQLPVAQRVYDRVKRAKLPDGVPDFRLSEAGGRDAALVFTRKSGKPLSEPLSGLFTAKGYRQGFLLASLNQAGTLAEEQWVLGRDQADQQNVASLAADVRRLYFQDYLRQWDALLADIDFVPITSVAQAADVLRILSGPTSPLKKLLVAVAKETDLQQEERLLAAQGQKVEGGVDQLKQRLGNLLGQEQSANPAAVASSEDPVSAHFAELNSLVSKGEGEPAAIDGLLADMNALYVQVSAMVGASGDALLGEAKNQASAAAARVSLTAERQPPVVQGLVKSVVGSTTNTMMGGVRNQLNAAWVSEVVNVYRQSLSGRYPMSPGSSRDATLEDFGQFFGTGGVMDNYFRKYLQPYVNTSTTTWSWQPGAAQKLGISPGVLQTFQRASNIRDAFFRSSGGTQPAVRFELKPVAMDANISQFLLDLDGQQLSYDHGPSRPVAMQWPNPGSIGVVRLSIMPPSATGRSGITLDGPWAWFRLLEQSDLTATNSPDRFNLRLRVDGASISYELRASSAFNPFKSRVLSGFSLPERL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9976 - -


  Protein sequence: 1169 a.a.    

>T6CP000275 NC_004129:c6907815-6904309 [Pseudomonas protegens Pf-5] [TssM]
VKAFFSFVIRWVIPLLGLIALSLIIWFIGPLLEFLVPEGRRWALIIFIFALWIGYRVFCIIQARRNAAKVLQSLAADTPP
DPDSVATAEELAALRQRMDEALALLKKAKLGGDERRNLYELPWYVIIGPPGSGKTTALVNSGLHFPLAAQLGAGAVRGVG
GTRNCDWWFTDQAVLLDTAGRYTTQDSHATVDKAAWLGFLDLLKTQRSRRPIDGAFVAISLSDLLLGSEAERAAHAAAIR
LRIQELYTQLGVRFPIYLMLTKLDLVPGFMEFFDSLSKEERAQVWGMTFALDDGKSGDSPLAHLPAELAALEQRLNERLV
ERLQQERDPARRDLIYGFPQQFAALKESLQSFLEGVFKPNAFEERVLLRGVYFTSGTQEGSPIDRLIGAMAQSMNLDRQH
LARQTGTGRSYFIEKLFSAVAFAERGLVGVNPKVERRRKWIARGALAGTVALVLVVSTLWIISYRANQAYIAQVDQRVGP
VRQDVQNLSPAQRDVLAVLPLLNATRHLAGDAPGWAEGLGLYQGDMLEAESASVYRKLLVAVFAPRLMTRIEEQLHSGGN
SDFLYEGLKAYLMLADSEHYDPDFIKAWITLDWDHTLARDLPPEQRQALTGHLQALFEKHPPNARLDQRLIDDLRRQLQQ
LPVAQRVYDRVKRAKLPDGVPDFRLSEAGGRDAALVFTRKSGKPLSEPLSGLFTAKGYRQGFLLASLNQAGTLAEEQWVL
GRDQADQQNVASLAADVRRLYFQDYLRQWDALLADIDFVPITSVAQAADVLRILSGPTSPLKKLLVAVAKETDLQQEERL
LAAQGQKVEGGVDQLKQRLGNLLGQEQSANPAAVASSEDPVSAHFAELNSLVSKGEGEPAAIDGLLADMNALYVQVSAMV
GASGDALLGEAKNQASAAAARVSLTAERQPPVVQGLVKSVVGSTTNTMMGGVRNQLNAAWVSEVVNVYRQSLSGRYPMSP
GSSRDATLEDFGQFFGTGGVMDNYFRKYLQPYVNTSTTTWSWQPGAAQKLGISPGVLQTFQRASNIRDAFFRSSGGTQPA
VRFELKPVAMDANISQFLLDLDGQQLSYDHGPSRPVAMQWPNPGSIGVVRLSIMPPSATGRSGITLDGPWAWFRLLEQSD
LTATNSPDRFNLRLRVDGASISYELRASSAFNPFKSRVLSGFSLPERL*

  Nucleotide sequence: 3507 bp    

>T6CP000275 NC_004129:c6907815-6904309 [Pseudomonas protegens Pf-5] [TssM]
GTGAAGGCGTTTTTCAGTTTTGTGATTCGCTGGGTGATCCCGCTGCTGGGGCTGATCGCCCTGAGCCTGATCATCTGGTT
CATTGGCCCGTTGCTGGAGTTCCTGGTACCGGAAGGCCGGCGTTGGGCGCTGATCATTTTCATCTTCGCGCTATGGATCG
GCTACCGGGTGTTTTGCATCATCCAGGCCCGTCGGAATGCGGCCAAGGTCTTGCAGAGCCTGGCCGCCGACACCCCGCCG
GACCCGGACAGCGTGGCCACTGCCGAGGAACTGGCCGCCCTGCGCCAGCGCATGGACGAAGCCCTGGCGCTGCTGAAGAA
GGCCAAGCTCGGCGGTGACGAGCGACGCAATCTCTACGAGCTGCCGTGGTACGTGATCATCGGTCCGCCGGGTTCCGGCA
AGACCACCGCACTGGTCAATTCCGGCCTGCATTTCCCCCTGGCCGCCCAGTTGGGCGCCGGTGCCGTACGCGGCGTGGGC
GGCACCCGCAACTGCGACTGGTGGTTCACCGACCAGGCCGTGCTGCTCGATACCGCCGGCCGCTACACCACTCAGGACAG
CCATGCCACGGTGGACAAGGCAGCCTGGCTGGGCTTTCTCGACCTGCTGAAAACCCAGCGCTCGCGGCGGCCGATCGATG
GCGCCTTCGTTGCCATCAGCCTCTCCGACCTGCTGCTCGGCAGCGAAGCGGAGCGCGCTGCCCACGCCGCAGCGATCCGC
CTGCGGATTCAAGAGCTGTACACCCAGTTGGGAGTGCGCTTCCCGATCTACCTGATGCTCACCAAGCTCGATCTGGTGCC
GGGTTTCATGGAGTTCTTCGACTCCCTGAGCAAGGAAGAGCGAGCCCAGGTCTGGGGCATGACCTTCGCCCTGGACGACG
GCAAGAGCGGTGACAGCCCCCTGGCCCACCTGCCGGCGGAACTGGCGGCCCTGGAGCAGCGGCTCAACGAGCGCCTGGTG
GAACGCCTGCAGCAGGAACGCGACCCGGCGCGGCGCGACCTGATCTACGGCTTTCCGCAGCAGTTCGCAGCGCTCAAGGA
ATCCTTGCAGAGCTTCCTGGAAGGGGTGTTCAAGCCCAATGCCTTCGAAGAGCGGGTACTGCTGCGCGGGGTGTACTTCA
CCAGCGGCACCCAGGAAGGCAGCCCGATCGACCGCCTGATCGGCGCCATGGCCCAGAGCATGAACCTGGACCGCCAGCAC
CTGGCGCGGCAGACCGGCACCGGGCGCAGCTACTTCATCGAGAAGCTGTTCAGTGCCGTGGCCTTTGCCGAGCGCGGGCT
GGTGGGGGTCAACCCCAAGGTCGAGCGCCGGCGCAAATGGATCGCCCGTGGCGCCCTGGCTGGCACCGTAGCCCTGGTGC
TGGTGGTCAGCACCTTGTGGATCATCAGCTACAGGGCCAACCAGGCCTACATTGCCCAGGTCGACCAGCGCGTCGGCCCG
GTGCGCCAGGACGTGCAGAACCTCAGCCCGGCCCAGCGCGATGTGCTGGCGGTGCTGCCGCTGCTCAACGCCACCCGCCA
CCTGGCCGGCGACGCCCCGGGCTGGGCCGAAGGCCTGGGCCTGTACCAGGGCGACATGCTCGAAGCCGAGTCCGCCAGCG
TCTATCGCAAGCTGCTGGTGGCGGTGTTCGCCCCACGGTTGATGACCCGCATCGAAGAGCAACTGCACAGCGGCGGCAAC
TCGGACTTCCTCTACGAGGGGCTCAAGGCCTACCTGATGCTGGCGGACAGCGAGCACTACGACCCGGACTTCATCAAGGC
CTGGATCACCCTGGACTGGGATCACACCCTGGCCCGCGACCTGCCGCCCGAGCAACGCCAGGCCCTGACCGGCCACCTGC
AGGCGCTGTTCGAGAAGCATCCGCCCAATGCGCGCCTGGACCAGCGCCTGATCGACGACCTGCGCCGCCAGCTGCAACAG
CTGCCGGTGGCCCAGCGGGTGTATGACCGGGTCAAGCGCGCCAAGCTGCCGGACGGCGTGCCGGACTTTCGCCTCAGCGA
GGCCGGTGGCCGTGACGCCGCGCTGGTGTTCACCCGCAAGAGCGGCAAGCCGTTGAGCGAGCCCCTGAGCGGGCTGTTTA
CCGCCAAGGGCTACCGCCAGGGCTTCCTCCTGGCCAGCCTCAACCAGGCAGGCACCCTGGCGGAAGAACAGTGGGTGCTG
GGTCGCGATCAGGCCGACCAGCAGAACGTCGCCAGCCTTGCCGCCGACGTGCGCCGCCTGTACTTCCAGGATTACCTGCG
CCAGTGGGACGCCCTGCTGGCGGACATCGACTTCGTGCCGATCACCAGCGTGGCCCAGGCCGCCGACGTGCTGCGGATTC
TTTCCGGGCCCACTTCGCCGCTGAAGAAGCTGCTGGTGGCCGTGGCCAAGGAAACCGACCTGCAACAGGAAGAACGCCTG
CTGGCGGCCCAGGGCCAGAAGGTCGAGGGCGGTGTGGACCAGCTCAAGCAACGCCTGGGTAACCTGCTGGGCCAGGAACA
GTCGGCCAACCCGGCTGCCGTGGCCAGCAGCGAAGACCCGGTGAGCGCGCACTTCGCCGAACTCAACAGCCTGGTGAGCA
AGGGCGAGGGCGAGCCGGCGGCCATCGACGGCCTGCTGGCGGACATGAACGCCCTGTACGTGCAGGTCAGCGCCATGGTC
GGAGCCAGCGGCGACGCGCTGCTGGGCGAGGCCAAGAACCAGGCCAGCGCCGCTGCGGCGCGGGTCAGCCTGACCGCCGA
GCGTCAGCCTCCGGTGGTCCAGGGGCTGGTCAAGTCGGTGGTGGGCTCCACCACCAACACCATGATGGGCGGGGTGCGCA
ACCAGCTGAACGCGGCCTGGGTCAGCGAAGTGGTGAACGTCTATCGCCAGTCCCTGAGCGGCCGTTACCCGATGTCGCCG
GGCAGCTCCCGGGACGCGACCCTGGAAGATTTCGGCCAGTTCTTCGGCACCGGCGGGGTGATGGACAACTACTTCCGCAA
GTACCTGCAGCCCTACGTCAACACCTCGACCACCACCTGGAGCTGGCAGCCAGGCGCGGCGCAGAAGCTGGGGATTTCCC
CTGGCGTGCTGCAGACCTTCCAGCGCGCTTCGAACATTCGCGACGCCTTCTTCCGCTCCAGTGGCGGTACCCAGCCGGCC
GTGCGTTTCGAACTCAAGCCGGTGGCGATGGACGCCAATATCAGCCAGTTCCTCCTCGACCTGGACGGCCAGCAACTGAG
CTACGACCACGGCCCGAGCCGGCCGGTGGCCATGCAATGGCCCAACCCGGGGAGCATCGGCGTGGTGCGCCTGTCGATCA
TGCCGCCTTCGGCCACCGGGCGTTCCGGGATCACCCTGGATGGCCCCTGGGCCTGGTTCCGCCTGCTGGAGCAGTCGGAC
CTGACCGCCACCAATTCGCCGGACCGCTTCAACCTGCGGCTACGGGTGGACGGTGCGAGCATTTCCTACGAGCTGCGGGC
CAGCAGCGCCTTCAACCCGTTCAAGAGCCGGGTGCTCAGTGGCTTCAGCCTGCCGGAGCGGCTATGA