Detailed information of component protein


Summary

Component ID T6CP000186
Component protein type TssI
T6SS ID (Type) T6SS00023 experimental (Type i3)
Strain Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Replicon chromosome
Sequence Protein sequence (730 a.a.); Nucleotide sequence (2190 bp)
Reference
[1] Blondel CJ, Jiménez JC, Contreras I, et al. Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 2009 Aug 4;10:354. doi: 10.1186/1471-2164-10-354. PMID: 19653904
[2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[3] Benz J, Reinstein J, Meinhart A. Structural Insights into the Effector - Immunity System Tae4/Tai4 from Salmonella typhimurium. PLoS One. 2013 Jun 27;8(6):e67362. doi: 10.1371/journal.pone.0067362. Print 2013. PMID: 23826277
[4] Sana TG, Flaugnatti N, Lugo KA, et al. Salmonella Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut. Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):E5044-51. doi: 10.1073/pnas.1608858113. Epub 2016 Aug 8. PMID: 27503894
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) STM0289
Coordinate (Strand) 329874..332063 (+)
NCBI ID NP_459287.1
RefSeq NC_003197
Uniprot ID Q8ZRL1_SALTY
KEGG ID stm:STM0289
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 6.5e-102 121..429
Phage_base_V PF04717.14 2.1e-13 484..551
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-729 MSFVSTNNKSGMGGLTTTTPPITGESGGVTADSVAGSVADAAESAVEQAAGSLFGALPEPSGLVKAAVAAAQAAAAAGMAQDAVSAIVSAVAGGPGAHNVTVSGSAVPPGALLFASLDGGETLSELFSYVVQLKTPDTLNLGYVSPAANLPLKPMVGKDLCVNIELDGGGKRHISGLVTAARVVGHEGRSVTYELRMEPWVKLLTHTSDYKAFQNKTVVDILDEVLAEYPYPVEKRLVESYPVRTWQVQYGETDFDFLQRLMQEWGIYWWFEHSEDSHTLVLADAISAHKACPDSPLVEWHQEGLKLDKEFIHTITANESLRTGQWVLDDFDFTKPRSLLANTVANPRETGHATYEHYEWPGDYFDKSEGEMLTRIRMEAQRSPGSRVLGGGNIRTLMTGYTFTLENYPTAEVNQEYLLMQTLLFVQDNAQHSGQDQHFTFSTRFELHPTREVFRPQRTVSKPHTKGPQSAIVTGPAGQEIWTDQYGRVKVQFGWDRYGKMDENSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGLPGMASQSGIFSHSLYGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVDRTKTIIKNETNSIGEDRNTTVTKNDGLSVKLAQTINIGTTYRLDVGDQFTLRCGNAALVLHKDGSIEFCGKQLMLHTSDVMQLIGKGIDMNPDGGTAVTADDIAPLLTSE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8920 - -


  Protein sequence: 730 a.a.    

>T6CP000186 NC_003197:329874-332063 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2] [TssI]
MSFVSTNNKSGMGGLTTTTPPITGESGGVTADSVAGSVADAAESAVEQAAGSLFGALPEPSGLVKAAVAAAQAAAAAGMA
QDAVSAIVSAVAGGPGAHNVTVSGSAVPPGALLFASLDGGETLSELFSYVVQLKTPDTLNLGYVSPAANLPLKPMVGKDL
CVNIELDGGGKRHISGLVTAARVVGHEGRSVTYELRMEPWVKLLTHTSDYKAFQNKTVVDILDEVLAEYPYPVEKRLVES
YPVRTWQVQYGETDFDFLQRLMQEWGIYWWFEHSEDSHTLVLADAISAHKACPDSPLVEWHQEGLKLDKEFIHTITANES
LRTGQWVLDDFDFTKPRSLLANTVANPRETGHATYEHYEWPGDYFDKSEGEMLTRIRMEAQRSPGSRVLGGGNIRTLMTG
YTFTLENYPTAEVNQEYLLMQTLLFVQDNAQHSGQDQHFTFSTRFELHPTREVFRPQRTVSKPHTKGPQSAIVTGPAGQE
IWTDQYGRVKVQFGWDRYGKMDENSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWG
LPGMASQSGIFSHSLYGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVDRTKTIIKNETNSIGEDRNTTV
TKNDGLSVKLAQTINIGTTYRLDVGDQFTLRCGNAALVLHKDGSIEFCGKQLMLHTSDVMQLIGKGIDMNPDGGTAVTAD
DIAPLLTSE*

  Nucleotide sequence: 2190 bp    

>T6CP000186 NC_003197:329874-332063 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2] [TssI]
ATGAGTTTTGTATCCACAAATAATAAATCCGGTATGGGAGGGCTGACGACAACCACGCCGCCGATAACCGGAGAAAGTGG
CGGTGTCACCGCAGATTCAGTCGCCGGAAGCGTGGCAGATGCGGCGGAATCCGCCGTGGAACAGGCTGCGGGATCGCTAT
TTGGCGCATTGCCGGAGCCATCAGGACTGGTGAAAGCCGCGGTAGCAGCGGCGCAGGCTGCCGCCGCCGCAGGTATGGCG
CAGGATGCGGTATCGGCCATCGTCTCTGCTGTTGCAGGCGGGCCGGGGGCGCATAATGTGACGGTCAGCGGCAGCGCCGT
ACCGCCGGGCGCATTACTGTTCGCCAGCCTGGACGGCGGCGAAACATTAAGTGAACTGTTCAGCTATGTGGTACAGCTAA
AAACGCCCGACACCCTGAATCTGGGCTATGTCTCCCCGGCGGCCAACCTGCCGCTCAAACCGATGGTGGGCAAAGATCTG
TGCGTCAACATCGAACTGGATGGTGGCGGTAAACGACATATCAGCGGGCTGGTCACGGCGGCGCGGGTGGTGGGCCATGA
AGGGCGTTCGGTTACCTATGAGCTGCGTATGGAGCCGTGGGTAAAACTGCTGACCCATACCAGCGACTACAAAGCATTCC
AGAATAAAACCGTGGTGGATATTCTGGATGAGGTTCTGGCGGAATATCCCTACCCGGTGGAAAAGCGGCTGGTGGAAAGC
TACCCGGTACGCACCTGGCAGGTGCAGTACGGTGAAACTGATTTTGATTTTCTTCAGCGACTGATGCAGGAGTGGGGCAT
CTACTGGTGGTTTGAGCACAGCGAGGACAGCCACACGCTGGTGCTGGCGGATGCCATCAGCGCCCACAAAGCATGTCCGG
ACTCGCCGCTGGTCGAGTGGCACCAGGAAGGGCTGAAGCTGGACAAGGAGTTTATCCACACTATCACGGCAAACGAGAGC
CTGCGGACTGGACAGTGGGTGCTGGATGATTTCGATTTTACGAAGCCACGTTCATTGCTGGCAAACACCGTGGCAAACCC
GCGTGAAACCGGTCATGCCACCTACGAGCATTATGAGTGGCCGGGAGACTACTTCGACAAGAGTGAAGGCGAGATGCTGA
CGCGCATTCGTATGGAAGCGCAGCGCAGCCCCGGCAGTCGGGTGCTGGGGGGAGGGAATATCCGCACACTCATGACCGGT
TATACCTTCACGCTGGAAAACTATCCCACCGCCGAAGTCAATCAGGAATATCTGCTGATGCAGACCTTGCTGTTTGTGCA
GGACAACGCGCAGCACAGCGGGCAGGACCAGCACTTTACCTTTTCCACCCGTTTTGAACTGCACCCCACCCGCGAGGTGT
TCCGCCCGCAGCGGACGGTGAGCAAACCCCACACCAAAGGGCCGCAGAGCGCCATCGTCACCGGCCCGGCGGGCCAGGAA
ATCTGGACGGATCAGTACGGGCGGGTAAAGGTACAGTTTGGTTGGGATCGCTACGGCAAAATGGATGAAAACAGCTCCTG
CTGGATACGCGTCAGCTACCCGTGGGCGGGCAAAGGCTTCGGGATGATCCAGATCCCGCGTATCGGCCAGGAAGTGCTGG
TGGATTTCAAAAACGGCGATCCGGATCTGCCGATCATCGTGGGGCGTACCTACAACCAGGACACCATGCCGCCGTGGGGA
CTGCCGGGAATGGCGTCGCAGAGCGGGATCTTCAGCCACTCGCTGTATGGCGGGCCAACGAACGGCAACATGCTGCGTTT
TGACGACAAAACGGGCGCGGAGGAAGTGAAGTTCCACGCGGAAAAAGATCTCAACACCACGGTGAAGAATAATGAAACGC
ATACGGTTATGGTGGATCGCACTAAAACCATTATTAAAAATGAAACCAACAGTATTGGTGAGGACAGAAACACCACGGTA
ACGAAGAATGACGGCCTTTCCGTAAAACTGGCGCAGACGATCAATATCGGCACCACTTATCGTTTAGATGTTGGCGATCA
ATTCACGCTTCGCTGCGGCAATGCGGCGCTTGTTTTACATAAGGACGGCTCCATTGAGTTTTGTGGCAAGCAACTGATGT
TACATACCAGCGATGTCATGCAACTGATTGGTAAAGGTATTGATATGAACCCGGATGGCGGCACAGCCGTAACCGCCGAT
GATATTGCCCCCCTTCTCACCTCTGAGTGA