Detailed information of component protein


Summary

Component ID T6CP000184
Component protein type TssL
T6SS ID (Type) T6SS00023 experimental (Type i3)
Strain Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Replicon chromosome
Sequence Protein sequence (435 a.a.); Nucleotide sequence (1305 bp)
Reference
[1] Blondel CJ, Jiménez JC, Contreras I, et al. Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 2009 Aug 4;10:354. doi: 10.1186/1471-2164-10-354. PMID: 19653904
[2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[3] Benz J, Reinstein J, Meinhart A. Structural Insights into the Effector - Immunity System Tae4/Tai4 from Salmonella typhimurium. PLoS One. 2013 Jun 27;8(6):e67362. doi: 10.1371/journal.pone.0067362. Print 2013. PMID: 23826277
[4] Sana TG, Flaugnatti N, Lugo KA, et al. Salmonella Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut. Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):E5044-51. doi: 10.1073/pnas.1608858113. Epub 2016 Aug 8. PMID: 27503894
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) STM0282
Coordinate (Strand) 321112..322416 (+)
NCBI ID NP_459280.1
RefSeq NC_003197
Uniprot ID Q93IS0_SALTY
KEGG ID stm:STM0282
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 3.2e-59 63..265
OmpA PF00691.22 5.3e-16 328..426
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-242 MTDSTLTPPAADMMSFLSTTPEHKDSEYETPVHTSQRTELNVIVEDGPDSKLRLAEISAAANPLLAAARPLLCALAAMPAKLDAALVEPYRNLLVREMHLYQTLCDQANLRREHVLAVRYCLCTALDEAANNTTWGRRGVWAGKSLLVTFHGESEGGIKLFQIIGRLAASFQEHGNVLEVIYHLLGLGFEGRYSVQPDGRKQLDNIRQQLLTQLSQRRDPVMPALSPDFQGAISGRLRRMRR
Transmembrane helix 243-265 VPVWLSAGIALLAMLTLFGLYSH
Inside 266-434 RMDVQTVTVQQHIDAIGIKLPPPPVPVHKLRLKILLANEIARGLLTVDEDDQHSRVVFRGDAMFVPGQKTVSDAIRPVINKAAREIARVGGAVTVTGHTDSQPIHSAEFPSNLVLSEKRAAEVAALLTSGGVPAGRVHIVGKGDTVPVADNGSKAGRAKNRRVEILVVE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9837 - -


  Protein sequence: 435 a.a.    

>T6CP000184 NC_003197:321112-322416 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2] [TssL]
MTDSTLTPPAADMMSFLSTTPEHKDSEYETPVHTSQRTELNVIVEDGPDSKLRLAEISAAANPLLAAARPLLCALAAMPA
KLDAALVEPYRNLLVREMHLYQTLCDQANLRREHVLAVRYCLCTALDEAANNTTWGRRGVWAGKSLLVTFHGESEGGIKL
FQIIGRLAASFQEHGNVLEVIYHLLGLGFEGRYSVQPDGRKQLDNIRQQLLTQLSQRRDPVMPALSPDFQGAISGRLRRM
RRVPVWLSAGIALLAMLTLFGLYSHRMDVQTVTVQQHIDAIGIKLPPPPVPVHKLRLKILLANEIARGLLTVDEDDQHSR
VVFRGDAMFVPGQKTVSDAIRPVINKAAREIARVGGAVTVTGHTDSQPIHSAEFPSNLVLSEKRAAEVAALLTSGGVPAG
RVHIVGKGDTVPVADNGSKAGRAKNRRVEILVVE*

  Nucleotide sequence: 1305 bp    

>T6CP000184 NC_003197:321112-322416 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2] [TssL]
ATGACAGACAGTACCCTGACGCCGCCAGCGGCGGATATGATGTCCTTTTTGTCCACCACGCCGGAACATAAGGACAGTGA
ATATGAAACGCCGGTACACACCAGCCAGCGCACGGAACTCAATGTCATCGTTGAAGACGGTCCGGACAGCAAACTCCGGC
TGGCTGAAATCAGCGCGGCGGCTAACCCGTTGCTCGCCGCTGCCCGGCCTTTATTGTGCGCTCTCGCAGCCATGCCCGCT
AAACTGGATGCGGCCCTGGTAGAGCCTTACCGTAATCTGCTGGTACGCGAGATGCATCTGTACCAGACATTATGCGATCA
GGCGAACCTGCGGCGCGAGCACGTACTGGCGGTACGTTACTGCCTGTGTACGGCGCTTGATGAAGCCGCCAATAACACAA
CCTGGGGACGGCGCGGCGTCTGGGCCGGAAAAAGCCTGCTGGTAACATTTCATGGTGAAAGCGAAGGCGGGATAAAACTT
TTCCAGATCATCGGGCGTCTGGCGGCCAGCTTCCAGGAGCATGGCAACGTACTGGAGGTTATCTACCACCTGCTGGGGTT
GGGATTTGAAGGCCGCTACAGCGTGCAGCCAGACGGGCGTAAGCAACTGGACAATATTCGCCAGCAACTGCTGACACAGC
TTTCACAGCGTCGCGATCCGGTTATGCCCGCGCTCTCGCCTGACTTTCAGGGGGCGATAAGCGGACGACTGCGGCGGATG
CGCCGGGTGCCGGTCTGGCTGAGCGCCGGGATAGCCCTGTTGGCGATGCTGACGCTGTTTGGCCTTTACAGCCACCGGAT
GGATGTGCAGACCGTCACCGTACAACAGCATATTGATGCGATTGGTATAAAACTGCCGCCGCCGCCTGTGCCGGTTCATA
AGCTGCGGCTGAAAATCCTGCTGGCAAACGAAATCGCCCGTGGCCTGCTGACCGTGGACGAAGATGACCAGCACAGTAGG
GTGGTCTTCCGTGGCGACGCCATGTTTGTGCCGGGACAGAAAACGGTGAGTGACGCAATCCGGCCAGTGATTAACAAAGC
GGCGCGGGAAATCGCCCGCGTGGGCGGCGCAGTCACTGTAACGGGTCACACTGACAGCCAGCCCATTCATTCGGCTGAAT
TCCCATCCAACCTGGTACTGTCGGAAAAACGGGCGGCGGAAGTTGCGGCGTTGCTGACCTCCGGCGGCGTACCTGCCGGA
CGGGTACATATCGTCGGCAAGGGCGATACGGTGCCGGTGGCGGATAACGGCAGTAAAGCCGGGCGGGCGAAAAACCGTCG
GGTGGAAATTCTGGTAGTGGAGTGA