Detailed information of component protein


Summary

Component ID T6CP000182
Component protein type TssJ
T6SS ID (Type) T6SS00023 experimental (Type i3)
Strain Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Replicon chromosome
Sequence Protein sequence (179 a.a.); Nucleotide sequence (537 bp)
Reference
[1] Blondel CJ, Jiménez JC, Contreras I, et al. Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 2009 Aug 4;10:354. doi: 10.1186/1471-2164-10-354. PMID: 19653904
[2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[3] Benz J, Reinstein J, Meinhart A. Structural Insights into the Effector - Immunity System Tae4/Tai4 from Salmonella typhimurium. PLoS One. 2013 Jun 27;8(6):e67362. doi: 10.1371/journal.pone.0067362. Print 2013. PMID: 23826277
[4] Sana TG, Flaugnatti N, Lugo KA, et al. Salmonella Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut. Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):E5044-51. doi: 10.1073/pnas.1608858113. Epub 2016 Aug 8. PMID: 27503894
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) STM0280
Coordinate (Strand) 319232..319768 (+)
NCBI ID NP_459278.1
RefSeq NC_003197
Uniprot ID Q8ZRL4_SALTY
KEGG ID stm:STM0280
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 6.3e-39 39..159
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-178 VNEWRNPTRWLCAVAMPFALLLLSGCGSSDALPDLESQRLDLSVKASDKVNPDNQKKAAPIEIRVYELKNDAAFTTADYWSLHDNDKSVLTDDLVRRDSFILRPGEEKKLRRPLNAQTTAIGVLAGYRNLAKSVWRVTYKIPEAPEKAWYSSFISGKGKVQLEAELEQSAIVITERDK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9989 pos: 25-26. LSG-CG. VNEWRNPTRWLCAVAMPFALLLLSG


  Protein sequence: 179 a.a.    

>T6CP000182 NC_003197:319232-319768 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2] [TssJ]
VNEWRNPTRWLCAVAMPFALLLLSGCGSSDALPDLESQRLDLSVKASDKVNPDNQKKAAPIEIRVYELKNDAAFTTADYW
SLHDNDKSVLTDDLVRRDSFILRPGEEKKLRRPLNAQTTAIGVLAGYRNLAKSVWRVTYKIPEAPEKAWYSSFISGKGKV
QLEAELEQSAIVITERDK*

  Nucleotide sequence: 537 bp    

>T6CP000182 NC_003197:319232-319768 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2] [TssJ]
GTGAATGAATGGCGTAACCCCACTCGGTGGTTATGTGCGGTAGCTATGCCTTTTGCACTGCTCCTGCTTTCCGGATGCGG
CAGTAGCGATGCGTTACCTGACCTCGAATCACAGCGACTCGACCTGAGCGTGAAAGCCTCCGATAAGGTGAATCCTGACA
ATCAGAAGAAGGCCGCGCCCATTGAGATACGTGTTTATGAACTGAAAAATGACGCCGCTTTCACGACAGCTGATTACTGG
TCGCTCCATGACAACGACAAATCCGTCCTTACCGACGATTTAGTGCGTCGCGACAGCTTTATTTTGCGTCCCGGCGAAGA
GAAAAAACTGCGTCGCCCGCTGAATGCGCAGACCACGGCAATCGGCGTACTGGCCGGATACCGTAACCTGGCCAAATCGG
TCTGGCGGGTAACCTACAAAATCCCGGAAGCCCCGGAAAAAGCCTGGTACAGCAGCTTCATATCGGGGAAAGGAAAAGTG
CAGTTGGAGGCGGAACTGGAACAAAGCGCCATTGTAATTACGGAACGGGATAAATGA