Detailed information of component protein


Summary

Component ID T6CP000177
Component protein type TssH
T6SS ID (Type) T6SS00023 experimental (Type i3)
Strain Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Replicon chromosome
Sequence Protein sequence (880 a.a.); Nucleotide sequence (2640 bp)
Reference
[1] Blondel CJ, Jiménez JC, Contreras I, et al. Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 2009 Aug 4;10:354. doi: 10.1186/1471-2164-10-354. PMID: 19653904
[2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[3] Benz J, Reinstein J, Meinhart A. Structural Insights into the Effector - Immunity System Tae4/Tai4 from Salmonella typhimurium. PLoS One. 2013 Jun 27;8(6):e67362. doi: 10.1371/journal.pone.0067362. Print 2013. PMID: 23826277
[4] Sana TG, Flaugnatti N, Lugo KA, et al. Salmonella Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut. Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):E5044-51. doi: 10.1073/pnas.1608858113. Epub 2016 Aug 8. PMID: 27503894
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) STM0272
Coordinate (Strand) 311195..313834 (+)
NCBI ID NP_459269.1
RefSeq NC_003197
Uniprot ID Q93IS9_SALTY
KEGG ID stm:STM0272
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 1.2e-38 609..776
AAA_lid_9 PF17871.3 1.7e-27 379..470
ClpB_D2-small PF10431.11 4.2e-12 783..857
AAA PF00004.31 4.8e-09 236..367
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-879 METPVSRSALYGKLAGPLFRSLESATAFCKLRSNPWVELTHWLHQLTQQPDNDILHVLRHYQIPLSDVEKALLRQLDMLPAGASAISDFSHHIDLSVEKAWMLASVRYGDNKIRSGWLLLALLTTPELRRVLSSICAPLATLPVDELTEILPSLIETSPEAQERPYDGSGLASAIPGESSQAIPNGGQDGKSALAKYCQDMTAQARDGKIDPVTGREHEIRTMTDILLRRRQNNPLLTGEAGVGKTAVVEGFALAIAQGEVPPALREVRLLALDVGALLAGASMKGEFESRLKGLLEEAGRSPQPVILFVDEVHTLVGAGGASGTGDAANLLKPALARGTLRTIGATTWSEYKRHIEKDPALTRRFQVLQIAEPEEIPAMEMVRGLVDTLEKHHNVLILDEAVRAAVQLSHRYIPARQLPDKAISLLDTAAARVALTLHTPPASVQFLRQQLKAAEMERSLLQKQEKMGIQSDERRDALMARIFSLNNELTASESRWQRELELVHTLQELRLAESDADDKTTLQQAETALREWQGDAPVVFPEVSAAVVAAIVADWTGIPAGRMVKDEASQVLELPARLAQRVTGQDGALAQIGERIQTARAGLGDPRKPVGVFMLAGPSGVGKTETALALAEAIYGGEQNLVTINMSEFQEAHTVSTLKGAPPGYVGYGEGGVLTEAVRRHPWSVVLLDEIEKAHHDVHELFYQVFDKGGMEDGEGTHVDFKNTTLLLTTNVGSDLISQMCEDPALMPDATGLKEALMPELRKHFPAAFLGRVTVIPYLPLDETSRGVIARLHLDRLVARMSEQHGVTLTYSEELVAHIVACCPMHETGARLLIGYIEQHILPRLSRYWLQAMTEKAAIRQIDIGVNGDEQIVFEIVC


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9720 - -


  Protein sequence: 880 a.a.    

>T6CP000177 NC_003197:311195-313834 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2] [TssH]
METPVSRSALYGKLAGPLFRSLESATAFCKLRSNPWVELTHWLHQLTQQPDNDILHVLRHYQIPLSDVEKALLRQLDMLP
AGASAISDFSHHIDLSVEKAWMLASVRYGDNKIRSGWLLLALLTTPELRRVLSSICAPLATLPVDELTEILPSLIETSPE
AQERPYDGSGLASAIPGESSQAIPNGGQDGKSALAKYCQDMTAQARDGKIDPVTGREHEIRTMTDILLRRRQNNPLLTGE
AGVGKTAVVEGFALAIAQGEVPPALREVRLLALDVGALLAGASMKGEFESRLKGLLEEAGRSPQPVILFVDEVHTLVGAG
GASGTGDAANLLKPALARGTLRTIGATTWSEYKRHIEKDPALTRRFQVLQIAEPEEIPAMEMVRGLVDTLEKHHNVLILD
EAVRAAVQLSHRYIPARQLPDKAISLLDTAAARVALTLHTPPASVQFLRQQLKAAEMERSLLQKQEKMGIQSDERRDALM
ARIFSLNNELTASESRWQRELELVHTLQELRLAESDADDKTTLQQAETALREWQGDAPVVFPEVSAAVVAAIVADWTGIP
AGRMVKDEASQVLELPARLAQRVTGQDGALAQIGERIQTARAGLGDPRKPVGVFMLAGPSGVGKTETALALAEAIYGGEQ
NLVTINMSEFQEAHTVSTLKGAPPGYVGYGEGGVLTEAVRRHPWSVVLLDEIEKAHHDVHELFYQVFDKGGMEDGEGTHV
DFKNTTLLLTTNVGSDLISQMCEDPALMPDATGLKEALMPELRKHFPAAFLGRVTVIPYLPLDETSRGVIARLHLDRLVA
RMSEQHGVTLTYSEELVAHIVACCPMHETGARLLIGYIEQHILPRLSRYWLQAMTEKAAIRQIDIGVNGDEQIVFEIVC*

  Nucleotide sequence: 2640 bp    

>T6CP000177 NC_003197:311195-313834 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2] [TssH]
ATGGAAACTCCTGTTTCACGCAGTGCGTTGTATGGAAAACTGGCCGGCCCACTATTCCGGTCGCTGGAATCGGCAACGGC
ATTTTGCAAACTACGCTCTAATCCCTGGGTTGAGCTGACTCACTGGCTGCACCAGTTAACACAGCAGCCCGATAACGATA
TTCTCCACGTTCTTCGGCATTACCAGATCCCTCTTTCTGATGTGGAGAAAGCGTTACTCCGGCAACTGGATATGCTGCCC
GCCGGGGCCAGCGCCATTAGTGATTTTTCTCACCATATCGATCTCAGCGTTGAAAAGGCCTGGATGCTGGCGAGCGTCCG
TTACGGCGATAACAAAATTCGCAGCGGCTGGTTGCTGCTGGCCTTGTTGACCACGCCAGAACTGCGTCGGGTACTGAGCA
GTATCTGCGCGCCGCTGGCCACGCTTCCGGTTGATGAACTGACGGAAATACTGCCCTCGTTGATCGAAACATCGCCGGAA
GCGCAGGAGCGCCCTTACGACGGCTCCGGCCTGGCATCAGCCATTCCCGGTGAAAGCAGTCAGGCGATTCCCAACGGCGG
GCAGGACGGTAAATCCGCGCTGGCAAAATACTGTCAGGACATGACGGCACAGGCGCGCGACGGCAAAATCGACCCGGTGA
CGGGGCGTGAGCATGAAATCCGCACCATGACGGATATTCTGCTGCGCCGTCGCCAGAATAATCCACTACTGACTGGTGAG
GCGGGCGTCGGGAAAACGGCGGTCGTCGAAGGTTTTGCCCTCGCGATTGCGCAGGGGGAAGTGCCGCCCGCGCTGCGGGA
AGTACGGCTACTGGCGCTGGACGTTGGCGCTCTGTTGGCCGGAGCCAGCATGAAAGGCGAGTTTGAATCGCGTCTGAAAG
GGTTACTGGAAGAGGCCGGGCGCTCGCCGCAGCCGGTTATTCTGTTTGTCGATGAAGTTCACACTCTGGTGGGCGCGGGC
GGCGCATCCGGCACGGGCGATGCCGCTAACCTGCTGAAACCGGCGCTGGCGCGCGGCACCCTGCGGACTATCGGCGCCAC
CACCTGGAGCGAATACAAGCGCCATATTGAGAAAGATCCGGCGCTGACCCGTCGTTTTCAGGTGTTGCAGATTGCCGAAC
CGGAAGAGATCCCCGCAATGGAAATGGTGCGTGGTCTGGTGGATACGCTGGAAAAACACCATAACGTACTGATTCTGGAT
GAGGCGGTACGTGCGGCGGTACAGCTTTCTCACCGCTACATTCCCGCCCGGCAGTTGCCGGATAAGGCCATCAGCCTGCT
GGATACCGCCGCGGCCCGCGTGGCGCTGACGCTGCACACGCCGCCTGCCAGCGTACAGTTCCTGCGCCAGCAGCTAAAAG
CGGCGGAAATGGAACGGTCGCTGTTGCAGAAGCAGGAAAAAATGGGGATTCAGTCAGATGAGCGGCGCGATGCGCTGATG
GCGCGAATTTTCTCGCTCAACAATGAACTGACTGCATCCGAATCCCGCTGGCAGCGGGAGCTGGAACTGGTACATACGTT
GCAGGAACTGCGTCTCGCAGAGTCTGATGCTGATGACAAAACCACGCTGCAACAGGCCGAAACGGCGCTAAGGGAGTGGC
AGGGCGACGCGCCGGTGGTGTTCCCGGAAGTCAGCGCGGCGGTTGTCGCGGCGATTGTCGCCGACTGGACCGGTATTCCT
GCTGGGCGCATGGTGAAAGATGAGGCCAGCCAGGTGCTGGAACTGCCTGCCCGACTGGCGCAACGCGTTACCGGGCAAGA
CGGCGCGCTGGCGCAGATTGGTGAACGTATTCAGACCGCCAGGGCGGGACTGGGCGATCCACGCAAACCGGTGGGCGTGT
TTATGCTGGCCGGGCCGTCCGGTGTCGGTAAAACCGAAACCGCGCTGGCGCTGGCGGAGGCTATCTACGGCGGCGAGCAG
AACCTGGTAACCATCAATATGAGCGAGTTCCAGGAGGCTCACACCGTTTCCACGCTGAAAGGCGCGCCGCCCGGCTATGT
GGGCTATGGCGAGGGTGGTGTGCTGACGGAAGCGGTGCGTCGCCACCCCTGGAGCGTAGTGCTGCTCGACGAGATCGAAA
AAGCGCACCATGACGTCCACGAACTCTTCTATCAGGTGTTTGACAAGGGTGGGATGGAGGACGGCGAGGGTACACATGTC
GATTTCAAAAACACCACGCTATTACTCACCACCAACGTGGGTTCCGACCTCATCAGCCAGATGTGTGAAGATCCGGCCTT
AATGCCCGATGCTACGGGGCTTAAAGAGGCGCTAATGCCGGAATTGCGCAAGCATTTCCCGGCGGCATTTCTGGGCCGCG
TGACGGTGATCCCTTACCTGCCGCTGGACGAAACGTCGCGTGGCGTGATTGCCCGTCTGCACCTTGACCGGCTGGTGGCG
CGGATGAGTGAACAGCACGGCGTGACGCTGACGTATAGCGAGGAACTGGTCGCACATATTGTGGCGTGCTGTCCAATGCA
TGAAACGGGCGCGCGGTTGCTGATTGGCTACATCGAACAGCACATTCTGCCACGACTGTCGCGCTACTGGTTGCAGGCCA
TGACGGAAAAAGCCGCGATCAGGCAGATTGATATCGGCGTTAATGGTGATGAGCAGATTGTTTTTGAGATCGTTTGCTGA