Detailed information of component protein


Summary

Component ID T6CP000175
Component protein type TssF
T6SS ID (Type) T6SS00023 experimental (Type i3)
Strain Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Replicon chromosome
Sequence Protein sequence (628 a.a.); Nucleotide sequence (1884 bp)
Reference
[1] Blondel CJ, Jiménez JC, Contreras I, et al. Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 2009 Aug 4;10:354. doi: 10.1186/1471-2164-10-354. PMID: 19653904
[2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[3] Benz J, Reinstein J, Meinhart A. Structural Insights into the Effector - Immunity System Tae4/Tai4 from Salmonella typhimurium. PLoS One. 2013 Jun 27;8(6):e67362. doi: 10.1371/journal.pone.0067362. Print 2013. PMID: 23826277
[4] Sana TG, Flaugnatti N, Lugo KA, et al. Salmonella Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut. Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):E5044-51. doi: 10.1073/pnas.1608858113. Epub 2016 Aug 8. PMID: 27503894
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) STM0268
Coordinate (Strand) 306724..308607 (-)
NCBI ID NP_459265.1
RefSeq NC_003197
Uniprot ID Q8ZRL9_SALTY
KEGG ID stm:STM0268
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 7.6e-223 1..624
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-627 MDPRLLEYYNRELSYLRETGAEFATLHPKIAARLGMQGTDIADPYVERMIEAFSFLSARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMAVVKLYPDTQEGDLAKGVTVPRDTAFVSPIPEGENTACQFRSSQDVTLWPLSIEEVRLTAAPPDMPALHRYLPPNIHVAGALRITLRTFGELTFSELAGPARLPFYLCGEERIASHLFELLHTSAVATLAGEPGHFDGELNVNLQHPVAHEGLEPGQGLLPLAWNVFHGHNLLHEFFACPERFYFFTPTGLSAGLQKVQGNVAEIVILLNRLPPDWLIHQTDAAQFSLFCTPVINLFPRTTTRIEVTHSVTEQHLVVDRTRPLDYEVFSVQEVEGLEAETTRKMIFRPLYHTRNNDEGNHGRYFSLRREPRRSSENARRYGTRTPYTGSEVFLSLVDQHEAPYPENLRHITVTAMVTNRDLPCLIPRNGRDDLTVDAAIPVAGVGLIRPPRPPQPPLAEREMAWRLIRQLSFNYLPLADLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVSCELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGHVMTWPVRTGQRGSV


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9938 - -


  Protein sequence: 628 a.a.    

>T6CP000175 NC_003197:c308607-306724 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2] [TssF]
MDPRLLEYYNRELSYLRETGAEFATLHPKIAARLGMQGTDIADPYVERMIEAFSFLSARTQLKIDAEFPRFTQRLLEVVS
PNYVTPTPSMAVVKLYPDTQEGDLAKGVTVPRDTAFVSPIPEGENTACQFRSSQDVTLWPLSIEEVRLTAAPPDMPALHR
YLPPNIHVAGALRITLRTFGELTFSELAGPARLPFYLCGEERIASHLFELLHTSAVATLAGEPGHFDGELNVNLQHPVAH
EGLEPGQGLLPLAWNVFHGHNLLHEFFACPERFYFFTPTGLSAGLQKVQGNVAEIVILLNRLPPDWLIHQTDAAQFSLFC
TPVINLFPRTTTRIEVTHSVTEQHLVVDRTRPLDYEVFSVQEVEGLEAETTRKMIFRPLYHTRNNDEGNHGRYFSLRREP
RRSSENARRYGTRTPYTGSEVFLSLVDQHEAPYPENLRHITVTAMVTNRDLPCLIPRNGRDDLTVDAAIPVAGVGLIRPP
RPPQPPLAEREMAWRLIRQLSFNYLPLADLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLV
YGRGVSCELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGHVMTWPVRTGQRGSV*

  Nucleotide sequence: 1884 bp    

>T6CP000175 NC_003197:c308607-306724 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2] [TssF]
ATGGATCCACGGCTACTGGAGTATTACAACCGCGAACTGAGCTACCTGCGGGAGACCGGTGCTGAGTTCGCCACCCTCCA
TCCCAAAATTGCCGCCCGACTGGGGATGCAGGGAACCGATATTGCCGACCCGTATGTGGAACGCATGATCGAGGCGTTCA
GCTTTCTGAGCGCCCGCACTCAGCTAAAAATTGACGCCGAGTTTCCCCGCTTCACACAGCGGTTACTGGAAGTCGTCAGC
CCCAATTACGTCACCCCCACGCCATCGATGGCAGTGGTAAAGCTGTATCCCGATACGCAGGAAGGCGACCTGGCGAAAGG
GGTCACCGTGCCGCGCGATACCGCGTTCGTTTCCCCCATCCCGGAAGGCGAAAATACCGCCTGCCAGTTTCGCAGCAGCC
AGGACGTCACGCTGTGGCCGCTGTCCATCGAGGAGGTACGCCTGACCGCCGCGCCGCCGGATATGCCCGCCCTGCACCGC
TATCTGCCGCCTAATATTCATGTCGCCGGGGCATTGCGCATCACCCTGCGAACCTTCGGGGAACTGACTTTCAGCGAGCT
GGCCGGTCCGGCACGGCTGCCCTTTTACCTCTGCGGCGAAGAACGCATTGCGTCACACCTGTTTGAGCTGCTACATACCA
GCGCCGTCGCCACACTGGCCGGTGAACCCGGTCACTTTGACGGCGAACTGAATGTGAATCTTCAACACCCGGTGGCGCAC
GAAGGGCTGGAGCCAGGGCAGGGGCTGCTACCGCTCGCCTGGAACGTGTTTCACGGCCATAATCTGCTGCATGAATTTTT
CGCCTGTCCGGAACGGTTTTACTTCTTCACCCCGACCGGATTGTCTGCCGGGTTACAAAAGGTGCAGGGCAATGTGGCGG
AAATTGTCATACTGCTTAACCGCCTGCCGCCGGACTGGCTGATTCATCAGACGGACGCTGCACAGTTCAGCCTGTTCTGT
ACGCCGGTGATCAACCTGTTTCCTCGCACCACCACCCGGATTGAAGTCACTCACAGCGTGACGGAACAGCATCTGGTGGT
GGATCGTACCCGTCCGCTGGATTACGAGGTGTTTTCTGTTCAGGAAGTCGAAGGCCTGGAAGCGGAAACCACACGGAAGA
TGATTTTTCGTCCGCTGTATCACACCCGTAATAATGACGAAGGCAACCACGGGCGTTATTTTTCACTGCGTCGTGAACCG
CGCCGCTCGTCAGAAAACGCCCGCCGCTATGGCACCCGAACCCCCTATACCGGCTCGGAAGTCTTCCTCTCGCTGGTTGA
TCAGCATGAAGCGCCCTACCCGGAAAATCTGCGCCATATCACCGTCACGGCAATGGTGACTAACCGCGATCTGCCCTGCC
TTATTCCACGTAATGGCCGGGATGATCTGACCGTAGATGCGGCGATTCCGGTGGCGGGCGTGGGTCTTATCAGACCGCCG
CGCCCGCCGCAGCCGCCGCTGGCTGAACGCGAAATGGCCTGGCGGCTGATCCGGCAGTTGTCGTTTAATTATCTGCCGCT
GGCCGATCTGGATCACCGCACCGGCGGCCAGGCGCTGCGCGATCTGCTGAATCTGTTTATCCCGGCTCACGACAGCCCGC
AGTCGCGCCAGGTGCGCAGCCTGATTGGCTGCAAAACCACCCCGGTGACCCGCCGCCTGCCCGGCTCCGGCCTGTTGGTC
TATGGCCGGGGCGTCAGTTGCGAACTGACCGTGGACGAAGAAGGATTTTCCGGTATCAGTCCTTACCTGTTCGGTCTGGT
GCTGGAACACTATATTGCCCGCCATGTTTCCATTAATACCTTCTCGCAGATGACGCTGCACTCGATGCAACGCGGGCATG
TCATGACCTGGCCGGTCAGAACCGGGCAACGGGGGAGCGTATGA