SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000110
Component protein type	TssG
T6SS ID (Type)	T6SS00015 (Type i1)
Strain	Pseudomonas putida KT2440
Replicon	chromosome
Sequence	Protein sequence (339 a.a.); Nucleotide sequence (1017 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537 [3] Bernal P, Allsopp LP, Filloux A, et al. The Pseudomonas putida T6SS is a plant warden against phytopathogens. ISME J. 2017 Apr;11(4):972-987. doi: 10.1038/ismej.2016.169. Epub 2017 Jan 3. PMID: 28045455

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PP_RS21175
Coordinate (Strand)	4606315..4607331 (+)
NCBI ID	WP_010954869.1
RefSeq	NC_002947
Uniprot ID	Q88FL2_PSEPK, A0A2V4EZW8_9PSED
KEGG ID	ppu:PP_4078
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	3.2e-80	20..321

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-338	MAHPDRQATADLADKLLANAHQYNFFQLLERLHGLHGDDLEPRWADEATRLRVRLASDPRLTFPVSDVYKAERMPGEDERYRVCTTFMGLHGTDSPLPTYYLEQVAYEHAQGVGVRPAFFDFFNHYLLSLLHRIWRKYRYYIRFQPGARDGFSQYMFALLGLNDKQLRGDTTLPWSRLLSFAGVIASRGRAPGTVAGIIAHCFDLTHVQIREFETRTVTTNSKQLVSLGRSNGELGSTFMVGSRTRTRSSKFTILISELDQAQLHDLLPSGINFGRLRALIDFLLRDGLAYDLELRLKQNALSPFYLHRSQGAYLGWTSFVDDHDGQISPVVRFRGRS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9956	-	-

Protein sequence: 339 a.a.

						>T6CP000110 NC_002947:4606315-4607331 [Pseudomonas putida KT2440] [TssG]
MAHPDRQATADLADKLLANAHQYNFFQLLERLHGLHGDDLEPRWADEATRLRVRLASDPRLTFPVSDVYKAERMPGEDER
YRVCTTFMGLHGTDSPLPTYYLEQVAYEHAQGVGVRPAFFDFFNHYLLSLLHRIWRKYRYYIRFQPGARDGFSQYMFALL
GLNDKQLRGDTTLPWSRLLSFAGVIASRGRAPGTVAGIIAHCFDLTHVQIREFETRTVTTNSKQLVSLGRSNGELGSTFM
VGSRTRTRSSKFTILISELDQAQLHDLLPSGINFGRLRALIDFLLRDGLAYDLELRLKQNALSPFYLHRSQGAYLGWTSF
VDDHDGQISPVVRFRGRS*					

Nucleotide sequence: 1017 bp

						>T6CP000110 NC_002947:4606315-4607331 [Pseudomonas putida KT2440] [TssG]
ATGGCCCATCCAGACCGGCAAGCAACCGCTGATCTAGCCGACAAGCTGCTCGCCAACGCGCACCAGTACAACTTCTTCCA
GTTGCTGGAGCGGTTGCATGGCCTGCACGGCGACGACCTGGAACCGCGTTGGGCGGATGAGGCCACCCGGCTGCGCGTGC
GGCTGGCCAGCGACCCGCGCCTGACCTTTCCCGTCTCTGATGTGTACAAGGCCGAGCGGATGCCCGGGGAGGATGAGCGC
TATCGCGTGTGCACCACGTTCATGGGGCTGCACGGTACCGACTCGCCCTTGCCCACCTATTACCTGGAACAGGTGGCCTA
TGAACATGCCCAGGGTGTAGGCGTGCGGCCGGCCTTCTTCGACTTCTTCAACCATTACCTGCTCAGCCTGCTGCACCGGA
TCTGGCGCAAGTACCGCTATTACATCCGTTTTCAACCCGGTGCGCGGGACGGGTTCTCCCAGTACATGTTCGCCCTGCTC
GGGCTGAACGACAAACAGCTGCGCGGCGACACGACACTGCCCTGGAGCCGCCTGCTCAGCTTTGCCGGTGTTATCGCCAG
CCGCGGCCGCGCACCGGGGACCGTCGCCGGCATCATCGCCCACTGCTTCGATCTGACGCACGTGCAGATCCGCGAATTCG
AGACGCGCACGGTCACCACCAACAGCAAACAGCTTGTAAGCCTCGGGCGCAGCAACGGCGAGCTTGGCAGCACGTTCATG
GTCGGCAGCCGCACCCGCACCCGCAGCAGCAAATTCACCATCCTCATCAGCGAACTCGACCAGGCGCAGCTGCATGACTT
GCTGCCCAGCGGCATCAACTTCGGACGCTTGCGCGCCTTGATTGATTTTCTGCTGCGCGATGGCCTGGCGTATGACCTGG
AGCTGCGCCTGAAACAAAACGCACTGTCGCCGTTCTACCTGCACCGCAGCCAAGGCGCCTACCTGGGCTGGACCAGCTTC
GTCGATGACCACGATGGCCAAATCAGTCCCGTTGTCCGCTTCCGGGGGCGGTCATGA					