Detailed information of component protein


Summary

Component ID T6CP000109
Component protein type TssF
T6SS ID (Type) T6SS00015 experimental (Type i1)
Strain Pseudomonas putida KT2440
Replicon chromosome
Sequence Protein sequence (589 a.a.); Nucleotide sequence (1767 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PP_RS21170
Coordinate (Strand) 4604585..4606351 (+)
NCBI ID WP_010954868.1
RefSeq NC_002947
Uniprot ID Q88FL3_PSEPK, A0A2V4F9S5_9PSED
KEGG ID ppu:PP_4077
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 7.2e-157 3..585
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-588 MSLKDRFSEELRYLHELGNDFAKDNPQLARLLGKAGSDPDVERLMEAFAFLTAKLRLKLEDDLPELTHPMLQILWPNYLRPLPSATIIRFSPRKQSLSQSQHIPKGARLFSKPVDAVPCEFRTCTGVSLHPFEISAVSATQTLDSSVVRIGLQTLVERPLNTLGCARLDFHLSGDNRTALTLYLWISQYLKHVSVIMNGEVRRLPANSIGFPGFSPEEALLPYPQNVFDGYRILQEYFVFPKRFHFFSITGLEKLWPAQACPQVGIEFHFTRQLPDTLRVGTEDFSLFCTPAVNLFKHSAEPIDLASEAAQVELKPRSEAQSAYEIFSVDEVISTRTTTDGSTGEHLRTFRPFESFAHEIEHVQGRTALYYRCQLEASLRGDGVTHRIAFVRADANSYIGELETASIDLTCTNRDLPLALGVDDINVLTEVTPPLATYTNICAPTRPYRPVLDGQLQWALISNMSLNYLSLLSVEPLKAVIRTYDFAALHDIQQARTTGKRLDGIRELHTQPMDWLIKGQPIRGLHTQLKLDQAAFLCEGDLYLFGCVLAHFFALYASINSFHQLEVINTTNNEHYTWPIQTGKQPLI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9953 - -


  Protein sequence: 589 a.a.    

>T6CP000109 NC_002947:4604585-4606351 [Pseudomonas putida KT2440] [TssF]
MSLKDRFSEELRYLHELGNDFAKDNPQLARLLGKAGSDPDVERLMEAFAFLTAKLRLKLEDDLPELTHPMLQILWPNYLR
PLPSATIIRFSPRKQSLSQSQHIPKGARLFSKPVDAVPCEFRTCTGVSLHPFEISAVSATQTLDSSVVRIGLQTLVERPL
NTLGCARLDFHLSGDNRTALTLYLWISQYLKHVSVIMNGEVRRLPANSIGFPGFSPEEALLPYPQNVFDGYRILQEYFVF
PKRFHFFSITGLEKLWPAQACPQVGIEFHFTRQLPDTLRVGTEDFSLFCTPAVNLFKHSAEPIDLASEAAQVELKPRSEA
QSAYEIFSVDEVISTRTTTDGSTGEHLRTFRPFESFAHEIEHVQGRTALYYRCQLEASLRGDGVTHRIAFVRADANSYIG
ELETASIDLTCTNRDLPLALGVDDINVLTEVTPPLATYTNICAPTRPYRPVLDGQLQWALISNMSLNYLSLLSVEPLKAV
IRTYDFAALHDIQQARTTGKRLDGIRELHTQPMDWLIKGQPIRGLHTQLKLDQAAFLCEGDLYLFGCVLAHFFALYASIN
SFHQLEVINTTNNEHYTWPIQTGKQPLI*

  Nucleotide sequence: 1767 bp    

>T6CP000109 NC_002947:4604585-4606351 [Pseudomonas putida KT2440] [TssF]
ATGTCACTGAAGGACAGATTCAGCGAAGAACTGCGTTACCTGCACGAGTTGGGAAACGACTTCGCCAAGGACAACCCCCA
GCTTGCCCGGCTGCTAGGCAAGGCGGGCAGCGACCCCGATGTCGAGCGGTTGATGGAAGCCTTTGCGTTTCTAACGGCCA
AGCTTCGGCTGAAGCTGGAAGATGACCTGCCAGAACTCACCCACCCCATGCTGCAGATACTGTGGCCCAACTACCTGCGC
CCATTGCCCAGCGCCACGATTATCCGATTCTCGCCACGCAAACAATCGCTCAGCCAGTCACAGCACATTCCCAAAGGTGC
AAGGTTGTTCTCGAAACCCGTGGACGCCGTGCCCTGCGAGTTTCGTACCTGCACGGGAGTGAGCCTTCATCCGTTTGAAA
TCAGCGCCGTCAGCGCAACCCAGACACTCGACAGCTCTGTGGTTCGCATCGGCCTGCAAACCTTGGTCGAACGGCCCCTG
AATACGTTGGGCTGCGCCCGTCTGGATTTCCACCTCAGCGGCGACAACCGCACCGCTCTGACGCTTTATCTATGGATTTC
TCAATACCTCAAACACGTCAGCGTGATCATGAACGGCGAGGTGCGCCGGCTACCGGCCAACAGCATCGGGTTCCCAGGTT
TCAGCCCTGAGGAGGCCTTGCTGCCCTACCCGCAGAACGTGTTCGATGGCTACCGTATTCTGCAGGAATACTTCGTGTTC
CCGAAGCGCTTCCACTTCTTCAGCATTACCGGCCTGGAAAAGCTGTGGCCGGCACAAGCCTGCCCACAGGTGGGCATCGA
GTTCCATTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCA
ACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCG
CAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCT
GCGGACTTTTCGCCCTTTCGAATCGTTCGCCCACGAAATCGAGCATGTCCAAGGCCGCACGGCGCTGTACTACCGCTGCC
AACTTGAAGCGTCCCTGCGCGGCGATGGCGTGACCCACCGCATCGCCTTCGTGCGCGCCGATGCCAACAGCTACATAGGC
GAGCTTGAAACTGCATCCATCGACCTGACCTGCACCAACAGGGACTTGCCGCTGGCGCTGGGCGTGGATGACATCAACGT
CCTCACCGAAGTCACGCCGCCCCTGGCGACCTATACCAACATCTGCGCCCCCACCCGCCCCTACCGGCCCGTGCTGGATG
GCCAGTTGCAGTGGGCGTTGATCTCCAACATGTCGCTCAACTACCTGTCGCTGTTGTCGGTCGAGCCGCTCAAGGCGGTG
ATTCGCACCTACGACTTCGCGGCGTTGCACGACATCCAGCAAGCGCGCACCACCGGCAAACGCCTCGACGGTATCCGCGA
ACTCCACACGCAGCCCATGGACTGGCTGATCAAGGGGCAACCCATACGAGGCCTGCACACCCAACTGAAGCTGGACCAGG
CGGCCTTCCTCTGCGAAGGCGACCTCTACCTGTTCGGCTGCGTGCTCGCGCACTTCTTCGCCCTGTACGCCAGCATCAAT
TCCTTCCATCAACTGGAAGTGATCAACACCACCAACAACGAGCACTACACATGGCCCATCCAGACCGGCAAGCAACCGCT
GATCTAG